Results 41 to 50 of about 16,155 (172)

Identification of Importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens [PDF]

, 2008
Background: The accurate normalization of differentially expressed genes in lung cancer is essential for the identification of novel therapeutic targets and biomarkers by real time RT-PCR and microarrays.
Nguewa, P.A. (Paul Alain), Agorreta, J. (Jackeline), Blanco, D. (D.), Lozano, M.D. (María Dolores), Gomez-Roman, J. (Javier), Sanchez, B.A. (Blas A.), Valles, I. (Iñaki), Pajares, M.J. (María José), Pio, R. (Rubén), Rodriguez, M.J. (María José), Montuenga-Badia, L.M. (Luis M.), Calvo-González, A. (Alfonso)   +11 more
core   +1 more source

Identification and evaluation of suitable reference genes for gene expression studies in the whitefly Bemisia tabaci (Asia I) by reverse transcription quantitative realtime PCR [PDF]

, 2014
This study presents a reliable method for performing reverse transcription quantitative realtime PCR (RT-qPCR) to measure gene expression in the whitefly Bemisia tabaci (Asia I) (Gennadius) (Hemiptera: Aleyrodidae), utilising suitable reference genes for
Carl Collins, David Bailey, Dinsdale A, John Colvin, Mitulkumar V. Patel, Scharlaken B, Susan Seal   +6 more
core   +1 more source

Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues

BMC Research Notes, 2011
Background Gene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in biological research due to the high-throughput and accuracy of qRT-PCR. For accurate and reliable gene expression analysis, normalization of gene expression
Looft Christian, Tesfaye Dawit, Cinar Mehmet, Uddin Muhammad, Tholen Ernst, Schellander Karl   +5 more
doaj   +1 more source

Loss of DET1 in High Pigment 2 Tomato Prevents High Temperature Repression of Anthocyanin Biosynthesis in Fruit Through HY5 Stabilization

Plant, Cell &Environment, EarlyView.
ABSTRACT Global warming impacts several aspects of plant physiology, with important negative effects on crop yield and production of secondary metabolites, such as anthocyanins. The anthocyanin content of vegetables and fruits has attracted public interest in the last two decades due to its health benefits, leading to the development of novel ...
Jacopo Menconi, Noemi La Monaca, Irene Cataldo, Pietro Maria Niccolini, Pierdomenico Perata, Silvia Gonzali   +5 more
wiley   +1 more source

Expression Stability of Reference Genes in Larvae of Spodoptera frugipferda Under Azadirachtin Stress by Real-Time Quantitative PCR Analysis

Guangdong nongye kexue
【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU, Yuting HUANG, Xuanyue YU, Cuiting LIU, Xinyi XIE, Hao SHEN, Jintian LIN   +6 more
doaj   +1 more source

Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of patients with Takayasu arteritis [PDF]

Jichu yixue yu linchuang, 2021
Objective To validate proper reference genes for quantitative real-time polymerase chain reaction (RT-qPCR) used for comparing mRNA expression levels in Takayasu arteritis' (TAK) and healthy controls' (HC) peripheral blood mononuclear cells (PBMC ...
TIAN Yi-xiao, LI Jing
doaj  

Validation of endogenous control genes for gene expression studies on human ocular surface epithelium. [PDF]

PLoS ONE, 2011
PURPOSE: To evaluate a panel of ten known endogenous control genes (ECG) with quantitative reverse transcription PCR (qPCR), for identification of stably expressed endogenous control genes in the ocular surface (OS) epithelial regions including cornea ...
Bina Kulkarni, Imran Mohammed, Andrew Hopkinson, Harminder Singh Dua   +3 more
doaj   +1 more source

Selection of miRNA reference genes for plant defence studies in rice (Oryza sativa)

, 2019
RT-qPCR is a widely used method to investigate the expression levels of genes under certain conditions. A key step, however, to have reliable results is the normalization of expression.
De Meyer, Tim, De Smet, Lien, Kyndt, Tina, Verstraeten, Bruno   +3 more
core   +1 more source

Circulating micrornas associated with glycemic impairment and progression in Asian Indians. [PDF]

, 2015
Aims/hypothesisAsian Indians have a high incidence of type 2 diabetes, but factors associated with glycemic progression in this population are not understood.
Aouizerat, Bradley E, Flowers, Elena, Gadgil, Meghana, Kanaya, Alka M   +3 more
core   +1 more source

Moderate maternal nutrient restriction alters type II alveolar epithelial cell density in the non‐human primate fetal lung

Experimental Physiology, EarlyView.
Abstract Restriction of fetal substrate supply has an adverse effect on surfactant maturation in the lung and thus affects the transition from in utero placental oxygenation to pulmonary ventilation ex utero. However, the consequences of reduced fetal substrate supply are dependent on the timing of gestation, severity and duration.
Mitchell C. Lock, Hillary F. Huber, Cun Li, Sandra Orgeig, Peter W. Nathanielsz, Janna L. Morrison   +5 more
wiley   +1 more source