Results 41 to 50 of about 16,273 (193)
Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]
, 2019 Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An +2 more
core +1 more source
Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata) [PDF]
, 2018 Background Cowpea (Vigna unguiculata) is an important source of protein supply for animal and human nutrition. The major storage globulins VICILIN and LEGUMIN (LEG) are synthesized from several genes including LEGA, LEGB, LEGJ and CVC (CONVICILIN).
Egea Gutiérrez-Cortines, Marcos +6 more
core +4 more sources
Annals of Neurology, EarlyView.Objective Traumatic brain injury (TBI) is an established risk factor for dementia, although the underlying mechanisms remain unclear. Our previous research demonstrated that a single severe TBI in wild‐type (WT) mice induces a prion‐like form of tau (tauTBI) that spreads throughout the brain, leading to memory deficits.
Gloria Vegliante +19 more
wiley +1 more source
BMC Research Notes, 2011 Background Gene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in biological research due to the high-throughput and accuracy of qRT-PCR. For accurate and reliable gene expression analysis, normalization of gene expression
Looft Christian +5 more
doaj +1 more source
Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR [PDF]
, 2009 Background: Considering the broad variation in the expression of housekeeping genes among tissues and experimental situations, studies using quantitative RT-PCR require strict definition of adequate endogenous controls.
Carlotti, Carlos G. +8 more
core +3 more sources
Plant Biotechnology Journal, EarlyView.ABSTRACT Microbial enzymes expressed in plants add new functionalities but occasionally trigger undesirable immune responses. Phanerochaete carnosa glucuronoyl esterase (PcGCE) hydrolyses the bond between lignin and 4‐O‐methyl‐α‐D‐glucuronic acid substituent of glucuronoxylan.
Evgeniy N. Donev +15 more
wiley +1 more source
Guangdong nongye kexue【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU +6 more
doaj +1 more source
Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of patients with Takayasu arteritis [PDF]
Jichu yixue yu linchuang, 2021 Objective To validate proper reference genes for quantitative real-time polymerase chain reaction (RT-qPCR) used for comparing mRNA expression levels in Takayasu arteritis' (TAK) and healthy controls' (HC) peripheral blood mononuclear cells (PBMC ...
TIAN Yi-xiao, LI Jing
doaj
Validation of endogenous control genes for gene expression studies on human ocular surface epithelium. [PDF]
PLoS ONE, 2011 PURPOSE: To evaluate a panel of ten known endogenous control genes (ECG) with quantitative reverse transcription PCR (qPCR), for identification of stably expressed endogenous control genes in the ocular surface (OS) epithelial regions including cornea ...
Bina Kulkarni +3 more
doaj +1 more source
Circulating micrornas associated with glycemic impairment and progression in Asian Indians. [PDF]
, 2015 Aims/hypothesisAsian Indians have a high incidence of type 2 diabetes, but factors associated with glycemic progression in this population are not understood.
Aouizerat, Bradley E +3 more
core +1 more source