Results 51 to 60 of about 16,155 (172)

Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA

BMC Research Notes, 2012
Background To obtain reliable quantitative real-time PCR data, normalization relative to stable housekeeping genes (HKGs) is required. However, in practice, expression levels of 'typical' housekeeping genes have been found to vary between tissues and ...
Cinar Mehmet, Islam Mohammad, Uddin Muhammad, Tholen Ernst, Tesfaye Dawit, Looft Christian, Schellander Karl   +6 more
doaj   +1 more source

Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey () tissues [PDF]

, 2008
Background: The rhesus monkey (Macaca mulatta) is a valuable and widely used model animal for biomedical research. However, quantitative analyses of rhesus gene expression profiles under diverse experimental conditions are limited by a shortage of ...
K Ahn, Jae Won Huh, Sang Je Park, Dae Soo Kim, H S Ha, Y J Kim, J R Lee, Kyu Tae Chang, H S Kim   +8 more
core   +1 more source

Human Brain Contusions Contain Pathogenic Transmissible Species that Induce Progressive Cognitive Decline and Tau Pathology in Mice

Annals of Neurology, Volume 99, Issue 4, Page 897-911, April 2026.
Objective Traumatic brain injury (TBI) is an established risk factor for dementia, although the underlying mechanisms remain unclear. Our previous research demonstrated that a single severe TBI in wild‐type (WT) mice induces a prion‐like form of tau (tauTBI) that spreads throughout the brain, leading to memory deficits.
Gloria Vegliante, Francesca Pischiutta, Elena Restelli, Federico Moro, Maria Antonietta Chiaravalloti, Ilaria Raimondi, Ilaria Bertani, Ilaria Lisi, Eliana Sammali, Rosaria Pascente, Serena Scozzari, Laura Pasetto, Carly Douglas, Marco Locatelli, Fabrizio Ortolano, Valentina Bonetto, David J. Loane, Nino Stocchetti, Roberto Chiesa, Elisa R. Zanier   +19 more
wiley   +1 more source

A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model

BMC Genomics, 2017
Background In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D ...
Felix Schulze, Deeksha Malhan, Thaqif El Khassawna, Christian Heiss, Anja Seckinger, Dirk Hose, Angela Rösen-Wolff   +6 more
doaj   +1 more source

Clonal Stabilization Reveals a DAO/3MST‐Expressing MDCK Subpopulation With Robust d‐Cysteine‐Mediated H2S Production

The FASEB Journal, Volume 40, Issue 6, 31 March 2026.
A subpopulation of MDCK (NBL‐2) cells, an epithelial cell line derived from the normal kidney, expresses DAO and 3MST. The DAO/3MST‐expressing cells are progressively eliminated during passaging; however, clonal isolation enables the establishment of sublines that maintain stable H2S production. Clonal cells co‐expressing DAO and 3MST therefore provide
Akari Miyamoto, Haruna Ueno, Hideo Kimura, Tadashi Nakagawa, Norihiro Shibuya   +4 more
wiley   +1 more source

Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

BMC Research Notes, 2019
Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome ...
Marcel Fernando da Silva, Marcos Cesar Gonçalves, Michael dos Santos Brito, Paula Macedo Nóbile, Larissa Mara de Andrade, Cibele Nataliane Medeiros, Silvana Creste, Luciana Rossini Pinto   +7 more
doaj   +1 more source

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An, Pasmans, Frank, Verbrugghe, Elin   +2 more
core   +1 more source

Silicon Nanoparticles Enhance Cold Tolerance in Elymus nutans Seedlings by Regulating Growth, Physiology, and Gene Expression Under Cold Stress

Food and Energy Security, Volume 15, Issue 2, March/April 2026.
ABSTRACT Cold stress represents one of the primary abiotic stresses affecting forage growth, development, yield, and quality. As a novel agricultural nanomaterial, silicon nanoparticles (SiNPs) demonstrate unique potential in regulating plant stress responses. However, their mechanism of action in forage cold responses remains poorly understood.
Yancui Zhao, Haoqin Liao, Huanhuan Lu, Liuban Tang, Yongsen Qiu, Ruofei Wang, Wengang Xie   +6 more
wiley   +1 more source

Reference gene validation for qPCR in rat carotid body during postnatal development [PDF]

, 2011
Background The carotid bodies are the main arterial oxygen chemoreceptors in mammals. Afferent neural output from the carotid bodies to brainstem respiratory and cardiovascular nuclei provides tonic input and mediates important protective responses to ...
Insook Kim, Dongjin Yang, Xinyu Tang, John L Carroll   +3 more
core   +2 more sources

Seed shattering in a North American Oryzeae grain: Developmental and genomic signatures of early domestication

The Plant Genome, Volume 19, Issue 1, March 2026.
Abstract Northern Wild Rice (NWR; Zizania palustris L.) is an aquatic grain endemic to North America and a member of the Oryzeae tribe. As an outcrossing crop with a short breeding history, domestication progress in cultivated NWR (cNWR) is ongoing, and seed shattering remains a major barrier to yield stability.
Reneth Millas, Lillian McGilp, Alan Mickelson, Maybell Banting, Claudia Castell‐Miller, Jennifer Kimball   +5 more
wiley   +1 more source