Results 51 to 60 of about 16,273 (193)

Loss of DET1 in High Pigment 2 Tomato Prevents High Temperature Repression of Anthocyanin Biosynthesis in Fruit Through HY5 Stabilization

Plant, Cell &Environment, EarlyView.
ABSTRACT Global warming impacts several aspects of plant physiology, with important negative effects on crop yield and production of secondary metabolites, such as anthocyanins. The anthocyanin content of vegetables and fruits has attracted public interest in the last two decades due to its health benefits, leading to the development of novel ...
Jacopo Menconi, Noemi La Monaca, Irene Cataldo, Pietro Maria Niccolini, Pierdomenico Perata, Silvia Gonzali   +5 more
wiley   +1 more source

Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA

BMC Research Notes, 2012
Background To obtain reliable quantitative real-time PCR data, normalization relative to stable housekeeping genes (HKGs) is required. However, in practice, expression levels of 'typical' housekeeping genes have been found to vary between tissues and ...
Cinar Mehmet, Islam Mohammad, Uddin Muhammad, Tholen Ernst, Tesfaye Dawit, Looft Christian, Schellander Karl   +6 more
doaj   +1 more source

Seed shattering in a North American Oryzeae grain: Developmental and genomic signatures of early domestication

The Plant Genome, Volume 19, Issue 1, March 2026.
Abstract Northern Wild Rice (NWR; Zizania palustris L.) is an aquatic grain endemic to North America and a member of the Oryzeae tribe. As an outcrossing crop with a short breeding history, domestication progress in cultivated NWR (cNWR) is ongoing, and seed shattering remains a major barrier to yield stability.
Reneth Millas, Lillian McGilp, Alan Mickelson, Maybell Banting, Claudia Castell‐Miller, Jennifer Kimball   +5 more
wiley   +1 more source

A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model

BMC Genomics, 2017
Background In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D ...
Felix Schulze, Deeksha Malhan, Thaqif El Khassawna, Christian Heiss, Anja Seckinger, Dirk Hose, Angela Rösen-Wolff   +6 more
doaj   +1 more source

Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]

Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus, Ali, Andersen, Bages, Bello, Bish, Breitbart, Brinkmeyer-Langford, Burton, Bustin, Bustin, Camerino, Cohn, Cotten, Craig, Crist, Duan, Falzarano, Feder, Fjeldbo, Flanigan, Galindo, Gong, Hildyard, Kallestad, Kornegay, Kornegay, Li, Liu, Mah, McGreevy, Nghiem, Pegoraro, Pfaffl, Piva, Reinig, Rho, Robinson-Hamm, Robriquet, Schmittgen, Solano, Spassov, Swaggart, Thellin, Vieira, Vieira Natassia, Walmsley, Webster, Wuebbles, Yu, Zammit   +50 more
core   +1 more source

Type I Interferon Gene Expression and Its Association With Ocular Involvement in Systemic Lupus Erythematosus

ACR Open Rheumatology, Volume 8, Issue 2, February 2026.
Objective Severalstudies have highlighted the role of Type I interferons (IFN‐I) in activating inflammatory pathways in lupus. However, no previous research focused on investigating the role of IFN‐I gene expression in the ophthalmologic involvement of systemic lupus erythematosus (SLE).
Raphael Teixeira Costa, Werbson Lima Guaraná, Braziliano Miguel da Silva Júnior, Jaciel Clementino, Luís Sousa Inês, Paula Sandrin‐Garcia, Thiago Sotero Fragoso   +6 more
wiley   +1 more source

Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

BMC Research Notes, 2019
Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome ...
Marcel Fernando da Silva, Marcos Cesar Gonçalves, Michael dos Santos Brito, Paula Macedo Nóbile, Larissa Mara de Andrade, Cibele Nataliane Medeiros, Silvana Creste, Luciana Rossini Pinto   +7 more
doaj   +1 more source

Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset [PDF]

, 2014
Background Gene expression analysis by reverse transcription quantitative PCR (qPCR) is the most widely used method for analyzing the expression of a moderate number of genes and also for the validation of microarray results.
Ana Viñas, Belén G Pardo, Diego Robledo, Jorge Hernández-Urcera, Laura Sánchez, Paulino Martínez, Rosa M Cal   +6 more
core   +4 more sources

Prognostic Value of Elevated miR‐502‐3p in Patients With Post‐Stroke Cognitive Impairment

Brain and Behavior, Volume 16, Issue 2, February 2026.
Significantly elevated serum miR‐502‐3p was positively correlated with both NIHSS and mRS scores, while it was negatively related to the MoCA score. Furthermore, the serum miR‐502‐3p was identified as a promising biomarker post‐stroke cognitive impairment (PSCI).
Yi Lin, Liang Xu, Yuhao Zhang, Cui Zou
wiley   +1 more source

Selection of miRNA reference genes for plant defence studies in rice (Oryza sativa)

, 2019
RT-qPCR is a widely used method to investigate the expression levels of genes under certain conditions. A key step, however, to have reliable results is the normalization of expression.
De Meyer, Tim, De Smet, Lien, Kyndt, Tina, Verstraeten, Bruno   +3 more
core   +1 more source