Results 81 to 90 of about 16,155 (172)

Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells

BMC Research Notes, 2013
Background The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this
Wei Ruoxin, Stewart Elizabeth Anne, Amoaku Winfried M   +2 more
doaj   +1 more source

Parallelism and divergence in immune responses: a comparison of expression levels in two lakes [PDF]

, 2014
Question: How do immune phenotypes differ between infected and uninfected wild individuals, and is the effect the same in different populations? Organisms: Threespine stickleback (Gasterosteus aculeatus) from two lake populations on the island of North ...
Ando, Ando, Anquetin, Anquetin, Baba, Bass, Berx, Berx, Berx, Birchmeier, Bracke, Brown, Chen, Christofori, Cukierman, David, Dias, Efstathiou, Gaul, Gaul, Gottlieb, Hedley, Ju, Lecomte, Machado, Maddess, Majchrzak, Morton, Morton, Munro, Nobis, Oskarsson, Reymond, Reymond, Risinger, Serrels, Shan, Sprague, Thiery, Timpson, Timpson, Trost, Umbas, Zhou   +43 more
core   +2 more sources

Glucuronoyl Esterase Expressed in Aspen Xylem Affects γ‐Ester Linkages Between Lignin and Glucuronoxylan Reducing Recalcitrance and Accelerating Growth

Plant Biotechnology Journal, Volume 23, Issue 12, Page 5417-5434, December 2025.
ABSTRACT Wood is the most abundant renewable natural resource composed of different polysaccharides and lignin, but its utilisation is hampered by intermolecular linkages between these components forming lignin‐carbohydrate complexes (LCCs) causing recalcitrance.
Marta Derba‐Maceluch, Laura García Romañach, Mattias Hedenström, Madhusree Mitra, Evgeniy N. Donev, János Urbancsok, Zakiya Yassin, Madhavi L. Gandla, Pramod Sivan, Jan Šimura, Gerhard Scheepers, Leif J. Jönsson, Francisco Vilaplana, Ewa J. Mellerowicz   +13 more
wiley   +1 more source

Elevated cortisol concentration in preterm sheep fetuses impacts heart development

Experimental Physiology, Volume 110, Issue 12, Page 1928-1947, December 1, 2025.
Abstract The prepartum rise in cortisol promotes cardiac development and maturation. Here, we investigated the impact of elevated circulating cortisol during mid‐late gestation on cardiac growth and metabolism in fetal sheep. Saline or cortisol (2–3 mg in 4.4 mL/24 h) was infused into the fetal jugular vein from 109 to 116 days gestation (dG, term ...
Reza Amanollahi, Stacey L. Holman, Melanie R. Bertossa, Ashley S. Meakin, Vicki L. Clifton, Kent L. Thornburg, I. Caroline McMillen, Michael D. Wiese, Mitchell C. Lock, Janna L. Morrison   +9 more
wiley   +1 more source

Evaluation of stable reference genes in white mustard (Sinapis alba) for qRT-PCR analysis under various stress conditions

The Indian Journal of Agricultural Sciences, 2020
White mustard (Sinapis alba L.) is a member of Brassicaceae family and is a source of various biotics stress resistance genes. S. alba is closely related to the cultivated Brassica juncea which exhibit susceptibility towards various pathogens ...
SHIKHA DIXIT, VINOD KUMAR JANGID, ANITA GROVER   +2 more
doaj   +1 more source

Identification of stably expressed reference small non-coding RNAs for microRNA quantification in high-grade serous ovarian carcinoma tissues [PDF]

, 2016
MicroRNAs (miRNAs) belong to a family of small non‐coding RNAs (sncRNAs) playing important roles in human carcinogenesis. Multiple investigations reported miRNAs aberrantly expressed in several cancers, including high‐grade serous ovarian carcinoma (HGS ...
Bandiera, Elisabetta, Bignotti, Eliana, Calza, Stefano, Odicino, Franco., Ravaggi, Antonella, Romani, Chiara, Sartori, Enrico, Tassi, Renata A., Todeschini, Paola, Zanotti, Laura   +9 more
core   +1 more source

EMT‐associated bias in the Parsortix® system observed with pancreatic cancer cell lines

Molecular Oncology, Volume 19, Issue 11, Page 3360-3375, November 2025.
The Parsortix® system was tested for CTC enrichment using pancreatic cancer cell lines with different EMT phenotypes. Spike‐in experiments showed lower recovery of mesenchymal‐like cells. This was confirmed with an EMT‐inducible breast cancer cell line.
Nele Vandenbussche, Renske Imschoot, Béatrice Lintermans, Lode Denolf, Joachim Taminau, Charlotte Fieuws, Geert Berx, Kris Gevaert, Kathleen B. M. Claes   +8 more
wiley   +1 more source

Integrative miRNOMe profiling reveals the miR‐195‐5p–CHEK1 axis and its impact on luminal breast cancer outcomes

Molecular Oncology, Volume 19, Issue 11, Page 3409-3426, November 2025.
In luminal (ER+) breast carcinoma (BC), miRNA profiling identified miR‐195‐5p as a key regulator of proliferation that targets CHEK1, CDC25A, and CCNE1. High CHEK1 expression correlates with worse relapse‐free survival after chemotherapy, especially in patients with luminal A subtype.
Veronika Boušková, Marie Ehrlichová, Alžběta Spálenková, Ivona Krus, Simona Šůsová, Viktor Hlaváč, Vlasta Němcová, Renata Koževnikovová, Markéta Trnková, David Vrána, Jiří Gatěk, Kateřina Kopečková, Marcela Mrhalová, Soňa Měšťáková, Pavel Souček   +14 more
wiley   +1 more source

Selection of stable reference genes in Prunus persica fruit infected with Monilinia laxa for normalisation of RT-qPCR gene expression data

Scientific Reports
Reverse transcription-quantitative PCR (RT-qPCR) is a powerful tool for quantifying gene expression. However, reference genes (RGs) for gene expression analysis in peach (Prunus persica) during interactions with Monilinia laxa, a major fungal pathogen ...
Agata Lizzio, Valerio Battaglia, Ernesto Lahoz, Massimo Reverberi, Milena Petriccione   +4 more
doaj   +1 more source

Testing reference genes for transcript profiling in Uromyces appendiculatus during urediospore infection of common bean.

PLoS ONE, 2020
Uromyces appendiculatus is a major pathogen on common bean. Like other rust fungi, it uses effectors to influence its host plant. Effectors are assumed to possess characteristic expression profiles, reflecting their activity during the infection process.
Tobias I Link
doaj   +1 more source