Results 191 to 200 of about 6,907,799 (304)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Publisher Correction: Greater trophic diversity of soil animal communities under agricultural land use and tropical climate. [PDF]

Nat Ecol Evol
Zhou Z, Eisenhauer N, Barnes AD, Pollierer MM, Jochum M, Grass I, Zhang Y, Brose U, Hyodo F, Scheunemann N, Schmidt O, Huang Y, Klarner B, Goncharov AA, Krause A, Korobushkin D, Gorbunova A, Lyubechanskii II, Tsurikov SM, Seeber J, Steinwandter M, Zryanin VA, Rozanova OL, Susanti WI, Crotty FV, Prameswari DA, Li Z, Melody C, Xie Z, Pan X, Wu D, Maraun M, Sam K, Tiunov AV, Scheu S, Potapov A.   +35 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Care delay during the COVID-19 pandemic in Germany - a cross-sectional online survey in the NAKO study. [PDF]

BMC Public Health
Massag J, Herrmann T, Pfrommer LR, Führer A, Berger K, Brenner H, De Santis KK, Greiser KH, Harth V, Karch A, Keil T, Khattak MNK, Klett-Tammen CJ, Krist L, Kristiansen NO, Lampl B, Leitzmann M, Lieb W, Mons U, Velásquez IM, Nieters A, Obi N, Övermöhle C, Peters A, Pischon T, Schmidt B, Schulze MB, Wirkner K, Zeeb H, Mikolajczyk R.   +29 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

FDG-PET/CT based small volume accelerated immuno chemoradiotherapy in locally advanced NSCLC (PACCELIO) - a randomized, open-label, multicenter phase II trial protocol. [PDF]

BMC Cancer
Shafie RE, Willmann J, Gkika E, Schimek-Jasch T, Miederer M, Bozorgmehr F, Griesinger F, Manapov F, Christopoulos P, Hehr T, Hecht M, Bütof R, Stuschke M, Guckenberger M, Schmid-Bindert G, Meiners J, Herzer S, Hartmann S, Lamché J, Rieken S, Nestle U.   +20 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Tumor mutations predict HER2-targeted therapy resistance in primary HER2-positive breast cancer. [PDF]

NPJ Breast Cancer
Van Mackelenbergh MT, Pfarr N, Weber K, Untch M, Solbach C, Schneeweiss A, Jank P, Blohmer J, Treue D, Schmatloch S, Lehmann A, Hanusch C, Link T, Sers C, Bjelic-Radisic V, Hummel M, Huober J, Schmitt WD, Fasching PA, Aktas B, Rhiem K, Reinisch M, Nekljudova V, Denkert C, Loibl S.   +24 more
europepmc   +1 more source

Germany

IMF Staff Country Reports, 2022
openaire   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source