Results 31 to 40 of about 340,349 (250)

ISOWN: accurate somatic mutation identification in the absence of normal tissue controls. [PDF]

, 2017
BackgroundA key step in cancer genome analysis is the identification of somatic mutations in the tumor. This is typically done by comparing the genome of the tumor to the reference genome sequence derived from a normal tissue taken from the same donor ...
Bartlett, John MS, Kalatskaya, Irina, McPherson, John D, Spears, Melanie, Stein, Lincoln, Trinh, Quang M   +5 more
core   +1 more source

Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the Caenorhabditis elegans Germline

G3: Genes, Genomes, Genetics, 2019
Identifying protein localization is a useful tool in analyzing protein function. Using GFP-fusion tags, researchers can study the function of endogenous proteins in living tissue.
Adam Hefel, Sarit Smolikove
doaj   +1 more source

Modulatory impact of Bifidobacterium longum subsp. longum BL21 on the gut–brain–ovary axis in polycystic ovary syndrome: insights into metabolic regulation, inflammation mitigation, and neuroprotection

mSphere
This study evaluates the efficacy of Bifidobacterium longum subsp. longum BL21 in mitigating symptoms of polycystic ovarian syndrome (PCOS) in DHT-induced PCOS model mice. It focuses on BL21’s role in modulating metabolic dysregulation, inflammation, and
Yao Dong, Shengnan Yang, Shu Zhang, Yuan Zhao, Xinlan Li, Mei Han, Zhonghui Gai, Kang Zou   +7 more
doaj   +1 more source

The dynamics of immunoglobulin V-gene usage and clonotype expansion in mice after prime and boost immunizations as analyzed by NGS

Human Vaccines & Immunotherapeutics, 2017
In the current study, an improved NGS approach was developed to study the B-cell repertoire evolution in a simple mouse immunization model including only two DNA immunizations.
Diego J. Farfán-Arribas, Shuying Liu, Shixia Wang, Shan Lu   +3 more
doaj   +1 more source

Cre-Recombinase Dependent Germline Deletion of a Conditional Allele in the Rgs9cre Mouse Line

Frontiers in Neural Circuits, 2018
Cre-LoxP conditional knockout animals have become a prominent tool to understand gene function in discrete cell-types and neural circuits. However, this technology has significant limitations including off target cre-dependent recombination.
Daniel J. Liput
doaj   +1 more source

The C. elegans gonadal sheath Sh1 cells extend asymmetrically over a differentiating germ cell population in the proliferative zone

eLife, 2022
The Caenorhabditis elegans adult hermaphrodite germline is surrounded by a thin tube formed by somatic sheath cells that support germ cells as they mature from the stem-like mitotic state through meiosis, gametogenesis, and ovulation.
Xin Li, Noor Singh, Camille Miller, India Washington, Bintou Sosseh, Kacy Lynn Gordon   +5 more
doaj   +1 more source

Germline and Somatic DNA Damage Repair Gene Mutations and Overall Survival in Metastatic Pancreatic Adenocarcinoma Patients Treated with FOLFIRINOX [PDF]

, 2018
Purpose: Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with lack of predictive biomarkers. We conducted a study to assess DNA damage repair (DDR) gene mutations as a predictive biomarker in PDAC patients treated with FOLFIRINOX ...
Gbolahan, Olumide, Hancock, Brad A., O'Neil, Bert H., Radovich, Milan, Sehdev, Amikar, Shahda, Safi, Stanley, Melissa, Wan, Jun, Wu, Howard H.   +8 more
core   +1 more source

Exploring Potential Germline-Associated Roles of the TRIM-NHL Protein NHL-2 Through RNAi Screening

G3: Genes, Genomes, Genetics, 2017
TRIM-NHL proteins are highly conserved regulators of developmental pathways in vertebrates and invertebrates. The TRIM-NHL family member NHL-2 in Caenorhabditis elegans functions as a miRNA cofactor to regulate developmental timing.
Gregory M. Davis, Wai Y. Low, Joshua W. T. Anderson, Peter R. Boag   +3 more
doaj   +1 more source

Innexin function dictates the spatial relationship between distal somatic cells in the Caenorhabditis elegans gonad without impacting the germline stem cell pool

eLife, 2022
Gap-junctional signaling mediates myriad cellular interactions in metazoans. Yet, how gap junctions control the positioning of cells in organs is not well understood. Innexins compose gap junctions in invertebrates and affect organ architecture. Here, we
Theadora Tolkin, Ariz Mohammad, Todd A Starich, Ken CQ Nguyen, David H Hall, Tim Schedl, E Jane Albert Hubbard, David Greenstein   +7 more
doaj   +1 more source

Differential expression of conserved germ line markers and delayed segregation of male and female primordial germ cells in a hermaphrodite, the leech helobdella. [PDF]

, 2013
In sexually reproducing animals, primordial germ cells (PGCs) are often set aside early in embryogenesis, a strategy that minimizes the risk of genomic damage associated with replication and mitosis during the cell cycle.
Cho, Sung-Jin, Vallès, Yvonne, WEISBLAT, David A.   +2 more
core   +2 more sources