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Cheap portable electroformed giant unilamellar vesicles preparation kit
Journal of Liposome Research, 2022The membrane of a cell separates the internal and external media of the cell and contributes to a variety of important processes, including gradient maintenance and signal transduction. Synthetic lipid-made vesicles are commonly utilized as cell membrane model systems.
Zendeh, Zahra +2 more
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Electroformation of Giant Unilamellar Vesicles: Investigating Vesicle Fusion versus Bulge Merging.
Langmuir, 2016Partially ordered stacks of phospholipid bilayers on a flat substrate can be obtained by the evaporation of a spread droplet of phospholipid-in-chloroform solution. When exposed to an aqueous buffer, numerous micrometric buds populate the bilayers, grow in size over minutes, and eventually detach, forming the so-called liposomes or vesicles.
Y. Micheletto +3 more
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Refined contour analysis of giant unilamellar vesicles
The European Physical Journal E, 2004The fluctuation spectrum of giant unilamellar vesicles is measured using a high-resolution contour detection technique. An analysis at higher q vectors than previously achievable is now possible due to technical improvements of the experimental setup and of the detection algorithm.
Pécréaux, J. +4 more
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Phase Transition Induced Adhesion of Giant Unilamellar Vesicles
ChemPhysChem, 2009AbstractCell and vesicle adhesion is believed to be dictated by the balance between a local interaction potential, which represents the sum of all attractive and repulsive forces and the elastic energy. Changing the mechanical properties of the membrane therefore offers a sensitive tool to control vesicle adhesion.
Frank, T. +3 more
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Methods for Forming Giant Unilamellar Fatty Acid Vesicles
2021Fatty acids readily assemble into bilayer membranes at a pH near their apparent pKa. Fatty acid vesicles are not only useful for research in the fields of origins of life, soft matter science, biophysics, and drug delivery, but are also cost-effective and easy to manipulate, making them ideal for teaching students about self-assembly and lipid bilayers.
Lauren A, Lowe +2 more
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Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles
Journal of Visualized Experiments, 2021Giant unilamellar vesicles (GUVs) are frequently used as models of biological membranes and thus are a great tool to study membrane-related cellular processes in vitro. In recent years, encapsulation within GUVs has proven to be a helpful approach for reconstitution experiments in cell biology and related fields. It better mimics confinement conditions
Bashirzadeh, Y. +4 more
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Electrofusion of giant unilamellar vesicles to cells
2015We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane.
Dikla, Raz-Ben Aroush +2 more
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Giant unilamellar vesicles with cytoskeleton
2019In this chapter we will give first an overview about the many functions of the cytoskeleton, its composition and structure in various organisms. We will describe next methods to anchor cytoskeletal filaments to membranes of supported lipid bilayers (SLB) and giant unilamellar vesicles (GUV) by several strategies based on charge, amphipathic helices ...
Härtel, T., Schwille, P.
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Acoustical deformability of giant unilamellar vesicles
The Journal of the Acoustical Society of America, 2017An acoustic standing wave is used to trap and deform giant unilamellar vesicles with a diameter ranging from 10 to 50 μm. The giant unilamellar vesicles are prepared in glucose solution with a bi-layer of DOPC membrane with approximately 10 nm-thickness. They are suspended in a 4 cm2-chamber of an acoustofluidic device.
Liangfei Tian +2 more
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Laser Tweezer Deformation of Giant Unilamellar Vesicles
2007Two methods are presented for deforming giant unilamellar vesicles with holographic optical tweezers. The first allows ultrahigh spatial- and temporal-resolution optical tracking of membrane deformations, by using embedded silica microspheres in a giant unilamellar vesicle as tracers.
Cory, Poole, Wolfgang, Losert
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