Results 161 to 170 of about 12,701 (197)

Electrofusion of giant unilamellar vesicles to cells

2015
We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane.
Dikla, Raz-Ben Aroush, Shlomit, Yehudai-Resheff, Kinneret, Keren   +2 more
openaire   +2 more sources

Giant unilamellar vesicles with cytoskeleton

2019
In this chapter we will give first an overview about the many functions of the cytoskeleton, its composition and structure in various organisms. We will describe next methods to anchor cytoskeletal filaments to membranes of supported lipid bilayers (SLB) and giant unilamellar vesicles (GUV) by several strategies based on charge, amphipathic helices ...
Härtel, T., Schwille, P.
openaire   +3 more sources

Acoustical deformability of giant unilamellar vesicles

The Journal of the Acoustical Society of America, 2017
An acoustic standing wave is used to trap and deform giant unilamellar vesicles with a diameter ranging from 10 to 50 μm. The giant unilamellar vesicles are prepared in glucose solution with a bi-layer of DOPC membrane with approximately 10 nm-thickness. They are suspended in a 4 cm2-chamber of an acoustofluidic device.
Liangfei Tian, Glauber T. Silva, Bruce W. Drinkwater   +2 more
openaire   +1 more source

Laser Tweezer Deformation of Giant Unilamellar Vesicles

2007
Two methods are presented for deforming giant unilamellar vesicles with holographic optical tweezers. The first allows ultrahigh spatial- and temporal-resolution optical tracking of membrane deformations, by using embedded silica microspheres in a giant unilamellar vesicle as tracers.
Cory, Poole, Wolfgang, Losert
openaire   +2 more sources

Interfacial Phases on Giant Unilamellar Vesicles

ASME 2012 Summer Bioengineering Conference, Parts A and B, 2012
Lipid nanodomains in cell membranes are believed to play a significant role in a number of critical cellular processes (Elson, et al., 2010). These include, for example, replication processes in enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer.
Yanfei Jiang, Guy M. Genin, Srikanth Singamaneni, Elliot L. Elson   +3 more
openaire   +1 more source

Deformation of giant unilamellar vesicles under osmotic stress

Colloids and Surfaces B: Biointerfaces, 2018
Biological membrane plays an important role in maintaining an osmotic equilibrium between the cytoplasm and the extracellular solution of cells. Here, the giant unilamellar vesicles (GUVs) as cell models were used to investigate the effect of osmotic stress on phospholipid membranes. The deformation of GUVs, including inward budding and outward budding,
Wei, Zong, Qingchuan, Li, Xunan, Zhang, Xiaojun, Han   +3 more
openaire   +2 more sources

Electroformation of giant unilamellar vesicles in saline solution

Colloids and Surfaces B: Biointerfaces, 2016
Giant unilamellar vesicle (GUV) formation on indium tin oxide (ITO) electrodes in saline solution and from charged lipids has proven to be difficult in the past. Yet the best cell membrane models contain charged lipids and require physiological conditions. We present a way to overcome this problem by using plasma cleaned ITO electrodes.
Qingchuan, Li, Xuejing, Wang, Shenghua, Ma, Ying, Zhang, Xiaojun, Han   +4 more
openaire   +2 more sources

Deformation Modes of Giant Unilamellar Vesicles Encapsulating Biopolymers

ACS Synthetic Biology, 2018
The shapes of giant unilamellar vesicles (GUVs) enclosing polymer molecules at relatively high concentration, used as a model cytoplasm, significantly differ from those containing only small molecules. Here, we investigated the effects of the molecular weights and concentrations of polymers such as polyethylene glycol (PEG), bovine serum albumin (BSA),
Taiji Okano, Koya Inoue, Kaoru Koseki, Hiroaki Suzuki   +3 more
openaire   +2 more sources

Giant Unilamellar Vesicle Microarrays for Cell Function Study

Analytical Chemistry, 2018
Giant unilamellar vesicles (GUVs) are widely used as artificial cell models which contribute to elucidate fundamental questions on origin of life and cell functions. Herein, the GUV microarrays were developed using a point-to-plane electrode system combined with microcontact stripping technique.
Chuntao Zhu, Qingchuan Li, Mingdong Dong, Xiaojun Han   +3 more
openaire   +3 more sources

Magnetically Controlled Polymer Giant Unilamellar Vesicles

Small
AbstractGiant unilamellar vesicles (GUVs) are essential tools for mimicking cellular processes such as membrane transport and for applications including sensing or protocell development. While it is often desirable to immobilize GUVs in these contexts, many immobilization approaches are irreversible, or limited in flexibility and scalability.
Narjes Abdollahi, Daniel Messmer, Voichita Mihali, Cornelia G. Palivan   +3 more
openaire   +1 more source