Results 151 to 160 of about 19,171 (192)

Effects of electrically-induced constant tension on giant unilamellar vesicles using irreversible electroporation

European Biophysics Journal, 2019
Mohammad Abu Sayem Karal, Md Kabir Ahamed, Marzuk Ahmed   +2 more
exaly   +2 more sources

Nanozyme-Powered Giant Unilamellar Vesicles for Mimicry and Modulation of Intracellular Oxidative Stress.

ACS Applied Materials and Interfaces, 2021
The bottom-up construction of enzyme-based artificial cells is generating increasing interest, but achieving artificial cells for "all artificial modules" remains challenging in synthetic biology.
Xueyan Hu, Xinyue Wang, Qiqi Liu, J. Wu, Haoqi Zhang, Anila Khalique, Zhiyuan Sun, Rui Chen, Jing Wei, Hongbo Li, D. Kong, Jie Zhuang, Xiyun Yan, Xinglu Huang   +13 more
semanticscholar   +1 more source

Refined contour analysis of giant unilamellar vesicles

The European Physical Journal E, 2004
The fluctuation spectrum of giant unilamellar vesicles is measured using a high-resolution contour detection technique. An analysis at higher q vectors than previously achievable is now possible due to technical improvements of the experimental setup and of the detection algorithm.
Pécréaux, J., Döbereiner, H.-G., Prost, J., Joanny, J.-F., Bassereau, P.   +4 more
openaire   +3 more sources

Electrofusion of giant unilamellar vesicles to cells

2015
We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane.
Dikla, Raz-Ben Aroush, Shlomit, Yehudai-Resheff, Kinneret, Keren   +2 more
openaire   +2 more sources

Electroformation of giant unilamellar vesicles in saline solution

Colloids and Surfaces B: Biointerfaces, 2016
Giant unilamellar vesicle (GUV) formation on indium tin oxide (ITO) electrodes in saline solution and from charged lipids has proven to be difficult in the past. Yet the best cell membrane models contain charged lipids and require physiological conditions. We present a way to overcome this problem by using plasma cleaned ITO electrodes.
Qingchuan, Li, Xuejing, Wang, Shenghua, Ma, Ying, Zhang, Xiaojun, Han   +4 more
openaire   +2 more sources

Giant unilamellar vesicles with cytoskeleton

2019
In this chapter we will give first an overview about the many functions of the cytoskeleton, its composition and structure in various organisms. We will describe next methods to anchor cytoskeletal filaments to membranes of supported lipid bilayers (SLB) and giant unilamellar vesicles (GUV) by several strategies based on charge, amphipathic helices ...
Härtel, T., Schwille, P.
openaire   +3 more sources

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir, 2021
Giant unilamellar vesicles (GUVs) adsorb to a solid surface and rupture to form a planar bilayer patch. These bilayer patches are used to investigate the properties and functions of biological membranes. Therefore, it is crucial to understand the mechanisms of GUV adsorption. In this study, we investigate the adsorption of phase-separated GUVs on glass
Chiho Kataoka-Hamai, Kohsaku Kawakami
openaire   +2 more sources

Advances in giant unilamellar vesicle preparation techniques and applications

Advances in Colloid and Interface Science, 2023
Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying membrane biophysical processes to acting as elementary blocks in the bottom-up assembly of synthetic cells.
Karthika S, Nair, Harsha, Bajaj
openaire   +2 more sources

Spatial effects of photosensitization on morphology of giant unilamellar vesicles

Biophysical Chemistry, 2021
Singlet oxygen (1O2) formed through photosensitization may initiate oxidative destruction of biomembranes, however, the influence from the spatial organization of photosensitizers (PS) relative to membranes remains unclear. To clarify this issue, we loaded riboflavin 5'-(dihydrogen phosphate) monosodium (FMN-Na) as a hydrophilic PS into the lumen of ...
Wei, Yuan, Zhou, Yi-Ming, Li, Yu-Qian, Gao, Rong-Yao, Fu, Li-Min, Wang, Peng, Zhang, Jian-Ping, Skibsted, Leif H.   +7 more
openaire   +3 more sources

Laser Tweezer Deformation of Giant Unilamellar Vesicles

2007
Two methods are presented for deforming giant unilamellar vesicles with holographic optical tweezers. The first allows ultrahigh spatial- and temporal-resolution optical tracking of membrane deformations, by using embedded silica microspheres in a giant unilamellar vesicle as tracers.
Cory, Poole, Wolfgang, Losert
openaire   +2 more sources