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Intravesicular Solute Delivery and Surface Area Regulation in Giant Unilamellar Vesicles Driven by Cycles of Osmotic Stresses. [PDF]
Sambre PD, Ho JCS, Parikh AN.
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Effects of Membrane Tension on Doxorubicin Uptake in Giant Unilamellar Vesicles
Sowon Kang
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Electrofusion of giant unilamellar vesicles to cells
2015We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane.
Dikla, Raz-Ben Aroush +2 more
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Spatial effects of photosensitization on morphology of giant unilamellar vesicles
Biophysical Chemistry, 2021Singlet oxygen (1O2) formed through photosensitization may initiate oxidative destruction of biomembranes, however, the influence from the spatial organization of photosensitizers (PS) relative to membranes remains unclear. To clarify this issue, we loaded riboflavin 5'-(dihydrogen phosphate) monosodium (FMN-Na) as a hydrophilic PS into the lumen of ...
Wei, Yuan +7 more
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Phase Transition Induced Adhesion of Giant Unilamellar Vesicles
ChemPhysChem, 2009AbstractCell and vesicle adhesion is believed to be dictated by the balance between a local interaction potential, which represents the sum of all attractive and repulsive forces and the elastic energy. Changing the mechanical properties of the membrane therefore offers a sensitive tool to control vesicle adhesion.
Frank, T. +3 more
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Advances in giant unilamellar vesicle preparation techniques and applications
Advances in Colloid and Interface Science, 2023Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying membrane biophysical processes to acting as elementary blocks in the bottom-up assembly of synthetic cells.
Karthika S, Nair, Harsha, Bajaj
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Giant unilamellar vesicles with cytoskeleton
2019In this chapter we will give first an overview about the many functions of the cytoskeleton, its composition and structure in various organisms. We will describe next methods to anchor cytoskeletal filaments to membranes of supported lipid bilayers (SLB) and giant unilamellar vesicles (GUV) by several strategies based on charge, amphipathic helices ...
Härtel, T., Schwille, P.
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Laser Tweezer Deformation of Giant Unilamellar Vesicles
2007Two methods are presented for deforming giant unilamellar vesicles with holographic optical tweezers. The first allows ultrahigh spatial- and temporal-resolution optical tracking of membrane deformations, by using embedded silica microspheres in a giant unilamellar vesicle as tracers.
Cory, Poole, Wolfgang, Losert
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Acoustical deformability of giant unilamellar vesicles
The Journal of the Acoustical Society of America, 2017An acoustic standing wave is used to trap and deform giant unilamellar vesicles with a diameter ranging from 10 to 50 μm. The giant unilamellar vesicles are prepared in glucose solution with a bi-layer of DOPC membrane with approximately 10 nm-thickness. They are suspended in a 4 cm2-chamber of an acoustofluidic device.
Liangfei Tian +2 more
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