Results 161 to 170 of about 6,262 (195)

Electrofusion of giant unilamellar vesicles to cells

2015
We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane.
Dikla, Raz-Ben Aroush   +2 more
openaire   +2 more sources

Spatial effects of photosensitization on morphology of giant unilamellar vesicles

Biophysical Chemistry, 2021
Singlet oxygen (1O2) formed through photosensitization may initiate oxidative destruction of biomembranes, however, the influence from the spatial organization of photosensitizers (PS) relative to membranes remains unclear. To clarify this issue, we loaded riboflavin 5'-(dihydrogen phosphate) monosodium (FMN-Na) as a hydrophilic PS into the lumen of ...
Wei, Yuan   +7 more
openaire   +3 more sources

Phase Transition Induced Adhesion of Giant Unilamellar Vesicles

ChemPhysChem, 2009
AbstractCell and vesicle adhesion is believed to be dictated by the balance between a local interaction potential, which represents the sum of all attractive and repulsive forces and the elastic energy. Changing the mechanical properties of the membrane therefore offers a sensitive tool to control vesicle adhesion.
Frank, T.   +3 more
openaire   +3 more sources

Advances in giant unilamellar vesicle preparation techniques and applications

Advances in Colloid and Interface Science, 2023
Giant unilamellar vesicles (GUVs) are versatile and promising cell-sized bio-membrane mimetic platforms. Their applications range from understanding and quantifying membrane biophysical processes to acting as elementary blocks in the bottom-up assembly of synthetic cells.
Karthika S, Nair, Harsha, Bajaj
openaire   +2 more sources

Giant unilamellar vesicles with cytoskeleton

2019
In this chapter we will give first an overview about the many functions of the cytoskeleton, its composition and structure in various organisms. We will describe next methods to anchor cytoskeletal filaments to membranes of supported lipid bilayers (SLB) and giant unilamellar vesicles (GUV) by several strategies based on charge, amphipathic helices ...
Härtel, T., Schwille, P.
openaire   +3 more sources

Laser Tweezer Deformation of Giant Unilamellar Vesicles

2007
Two methods are presented for deforming giant unilamellar vesicles with holographic optical tweezers. The first allows ultrahigh spatial- and temporal-resolution optical tracking of membrane deformations, by using embedded silica microspheres in a giant unilamellar vesicle as tracers.
Cory, Poole, Wolfgang, Losert
openaire   +2 more sources

Acoustical deformability of giant unilamellar vesicles

The Journal of the Acoustical Society of America, 2017
An acoustic standing wave is used to trap and deform giant unilamellar vesicles with a diameter ranging from 10 to 50 μm. The giant unilamellar vesicles are prepared in glucose solution with a bi-layer of DOPC membrane with approximately 10 nm-thickness. They are suspended in a 4 cm2-chamber of an acoustofluidic device.
Liangfei Tian   +2 more
openaire   +1 more source

Home - About - Disclaimer - Privacy