Results 211 to 220 of about 40,389 (237)
Facile generation of giant unilamellar vesicles using polyacrylamide gels. [PDF]
Sci Rep, 2020 Parigoris E +7 more
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Development of supported biomimetic membranes for insertion of aquaporin protein water channels for novel water filtration applications [PDF]
, 2011 Hansen, Jesper Søndergaard
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Protein Reconstitution Inside Giant Unilamellar Vesicles
Annual Review of Biophysics, 2021Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light microscopic investigation of dynamic processes in and on membranes.
Petra Schwille, Thomas Litschel
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Microdomain evolution on giant unilamellar vesicles
Biomechanics and Modeling in Mechanobiology, 2012A chemo-mechanical model is used to capture the formation and evolution of microdomains on the deforming surface of giant unilamellar vesicles. The model is intended for the regime of vesicle dynamics characterized by a distinct difference in time scales between shape change and species transport.
John E. Dolbow, Eliot Fried, Anand Embar
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Refined contour analysis of giant unilamellar vesicles
The European Physical Journal E, 2004The fluctuation spectrum of giant unilamellar vesicles is measured using a high-resolution contour detection technique. An analysis at higher q vectors than previously achievable is now possible due to technical improvements of the experimental setup and of the detection algorithm.
Pécréaux, J. +4 more
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Starch aided synthesis of giant unilamellar vesicles
Chemistry and Physics of Lipids, 2020Synthesis of giant unilamellar vesicles (GUVs) of charged and uncharged lipids at physiological salt concentration is presented using the starch hydrogel method as an example of the gel assisted synthesis method. The swelling of the gel is assisted by the presence of a high amount of amylopectin in starch and yields giant-sized vesicles, which are ...
Rochish Thaokar +2 more
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Trapping and release of giant unilamellar vesicles in microfluidic wells
Soft Matter, 2014We describe the trapping and release of giant unilamellar vesicles (GUVs) in a thin and wide microfluidic channel, as they cross indentations etched in the channel ceiling. This trapping results from the reduction of the membrane elastic energy, which is stored in the GUV as it squeezes to enter into the thin channel.
Yamada, Ayako +3 more
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Electrofusion of giant unilamellar vesicles to cells
2015We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane.
Dikla Raz-Ben Aroush +2 more
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Electroformation of giant unilamellar vesicles in saline solution
Colloids and Surfaces B: Biointerfaces, 2016Giant unilamellar vesicle (GUV) formation on indium tin oxide (ITO) electrodes in saline solution and from charged lipids has proven to be difficult in the past. Yet the best cell membrane models contain charged lipids and require physiological conditions. We present a way to overcome this problem by using plasma cleaned ITO electrodes.
Xuejing Wang +4 more
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Acoustical deformability of giant unilamellar vesicles
The Journal of the Acoustical Society of America, 2017An acoustic standing wave is used to trap and deform giant unilamellar vesicles with a diameter ranging from 10 to 50 μm. The giant unilamellar vesicles are prepared in glucose solution with a bi-layer of DOPC membrane with approximately 10 nm-thickness. They are suspended in a 4 cm2-chamber of an acoustofluidic device.
Glauber T. Silva +2 more
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