Results 221 to 230 of about 40,389 (237)
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Laser Tweezer Deformation of Giant Unilamellar Vesicles
2007Two methods are presented for deforming giant unilamellar vesicles with holographic optical tweezers. The first allows ultrahigh spatial- and temporal-resolution optical tracking of membrane deformations, by using embedded silica microspheres in a giant unilamellar vesicle as tracers.
Cory Poole, Wolfgang Losert
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Methods for Forming Giant Unilamellar Fatty Acid Vesicles
2021Fatty acids readily assemble into bilayer membranes at a pH near their apparent pKa. Fatty acid vesicles are not only useful for research in the fields of origins of life, soft matter science, biophysics, and drug delivery, but are also cost-effective and easy to manipulate, making them ideal for teaching students about self-assembly and lipid bilayers.
Lauren A Lowe, Anna Wang, Daniel W K Loo
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Interfacial Phases on Giant Unilamellar Vesicles
ASME 2012 Summer Bioengineering Conference, Parts A and B, 2012Lipid nanodomains in cell membranes are believed to play a significant role in a number of critical cellular processes (Elson, et al., 2010). These include, for example, replication processes in enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer.
Guy M. Genin +3 more
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Phase Transition Induced Adhesion of Giant Unilamellar Vesicles
ChemPhysChem, 2009AbstractCell and vesicle adhesion is believed to be dictated by the balance between a local interaction potential, which represents the sum of all attractive and repulsive forces and the elastic energy. Changing the mechanical properties of the membrane therefore offers a sensitive tool to control vesicle adhesion.
Achim Wixforth +4 more
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Spatial effects of photosensitization on morphology of giant unilamellar vesicles
Biophysical Chemistry, 2021Singlet oxygen (1O2) formed through photosensitization may initiate oxidative destruction of biomembranes, however, the influence from the spatial organization of photosensitizers (PS) relative to membranes remains unclear. To clarify this issue, we loaded riboflavin 5'-(dihydrogen phosphate) monosodium (FMN-Na) as a hydrophilic PS into the lumen of ...
Wei, Yuan +7 more
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Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass
Langmuir, 2021Giant unilamellar vesicles (GUVs) adsorb to a solid surface and rupture to form a planar bilayer patch. These bilayer patches are used to investigate the properties and functions of biological membranes. Therefore, it is crucial to understand the mechanisms of GUV adsorption. In this study, we investigate the adsorption of phase-separated GUVs on glass
Chiho Kataoka-Hamai, Kohsaku Kawakami
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Giant Unilamellar Vesicle Microarrays for Cell Function Study
Analytical Chemistry, 2018Giant unilamellar vesicles (GUVs) are widely used as artificial cell models which contribute to elucidate fundamental questions on origin of life and cell functions. Herein, the GUV microarrays were developed using a point-to-plane electrode system combined with microcontact stripping technique.
Chuntao Zhu +3 more
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Giant unilamellar vesicle formation under physiologically relevant conditions
Chemistry and Physics of Lipids, 2008We present an upgrade to the giant unilamellar vesicle (GUV) electroformation method allowing easy GUV production in different buffers and with various membrane compositions. Our experimental results reveal that lipid deposits obtained from aqueous liposome or proteoliposome dispersions are highly efficient for GUV electroformation.
Hélène Bouvrais +3 more
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Orientation of membrane probes in giant unilamellar vesicles
SPIE Proceedings, 2005Many biological processes are accompanied by changes in the orientation of membrane-embedded molecules. Fluorescence polarization microscopy (FPM) is an optical technique that can be used to determine how the transition dipole of a fluorophore is oriented with respect to the membrane and therefore may detect these changes. The FPM experimental setup is
Robert M. Raphael, Jennifer N. Greeson
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Deformation of giant unilamellar vesicles under osmotic stress
Colloids and Surfaces B: Biointerfaces, 2018Biological membrane plays an important role in maintaining an osmotic equilibrium between the cytoplasm and the extracellular solution of cells. Here, the giant unilamellar vesicles (GUVs) as cell models were used to investigate the effect of osmotic stress on phospholipid membranes. The deformation of GUVs, including inward budding and outward budding,
Wei Zong +3 more
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