Results 21 to 30 of about 579,153 (203)

Giant vesicles in electric fields [PDF]

open access: yesSoft Matter, 2007
This review is dedicated to electric field effects on giant unilamellar vesicles, a cell-size membrane system. We summarize various types of behavior observed when vesicles are subjected either to weak AC fields at various frequency, or to strong DC pulses.
Dimova, R.   +5 more
openaire   +3 more sources

Dynamic monitoring of a bi-enzymatic reaction at a single biomimetic giant vesicle.

open access: yesIn Analysis, 2020
Giant unilamellar vesicles were used as individual biomimetic micro-reactors wherein a model bi-enzymatic reaction involving a glucose oxidase (GOx) and horseradish peroxidase (HRP) was monitored by confocal microscopy.
Pauline Lefrançois   +2 more
semanticscholar   +1 more source

Evolution of Proliferative Model Protocells Highly Responsive to the Environment

open access: yesLife, 2022
In this review, we discuss various methods of reproducing life dynamics using a constructive approach. An increase in the structural complexity of a model protocell is accompanied by an increase in the stage of reproduction of a compartment (giant ...
Muneyuki Matsuo   +3 more
doaj   +1 more source

Asymmetric Giant Unilamellar Vesicles [PDF]

open access: yesBiophysical Journal, 2010
The lipid composition of the eukaryotic plasma membrane is asymmetric; that is, if the bilayer is considered to consist of two leaflets, the outer-facing leaflet contains different lipids at different concentrations than the inner-facing leaflet. While there has been much speculation as to the physiological purpose of this asymmetry, it has been ...
Hu, Peichi C., Malmstadt, Noah
openaire   +1 more source

Environment-Sensitive Intelligent Self-Reproducing Artificial Cell with a Modification-Active Lipo-Deoxyribozyme

open access: yesMicromachines, 2020
As a supramolecular micromachine with information flow, a giant vesicle (GV)-based artificial cell that exhibits a linked proliferation between GV reproduction and internal DNA amplification has been explored in this study.
Muneyuki Matsuo   +6 more
doaj   +1 more source

A simple method to make, trap and deform a vesicle in a gel

open access: yesScientific Reports, 2023
We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double ...
Pierre Tapie   +4 more
doaj   +1 more source

Using Imaging Flow Cytometry to Quantify and Optimize Giant Vesicle Production by Water-in-oil Emulsion Transfer Methods.

open access: yesLangmuir, 2019
Many biologists, biochemists, and biophysicists study giant vesicles, which have a diameter of >1 μm, owing to their ease of characterization using standard optical methods.
Yuka Matsushita-Ishiodori   +4 more
semanticscholar   +1 more source

Physical Concept to Explain the Regulation of Lipid Membrane Phase Separation under Isothermal Conditions

open access: yesLife, 2023
Lateral phase separation within lipid bilayer membranes has attracted considerable attention in the fields of biophysics and cell biology. Living cells organize laterally segregated compartments, such as raft domains in an ordered phase, and regulate ...
Naofumi Shimokawa, Tsutomu Hamada
doaj   +1 more source

Colocalization Analysis of Lipo-Deoxyribozyme Consisting of DNA and Protic Catalysts in a Vesicle-Based Protocellular Membrane Investigated by Confocal Microscopy

open access: yesLife, 2021
The linkage between the self-reproduction of compartments and the replication of DNA in a compartment is a crucial requirement for cellular life.
Yuiko Hirata   +5 more
doaj   +1 more source

Formation of Oligovesicular Vesicles by Micromanipulation

open access: yesMembranes, 2011
Cell-sized lipid bilayer membrane vesicles (giant vesicles, GVs) or semi-vesicles were formed from egg yolk phosphatidylcholine on a platinum electrode under applied electric voltage by electroformation.
Yukihisa Okumura   +2 more
doaj   +1 more source

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