Results 31 to 40 of about 579,153 (203)

Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network

open access: yesBMC Bioinformatics, 2022
Background Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces.
Il-Hyung Lee   +4 more
doaj   +1 more source

Lipid vesicle shape analysis from populations using light video microscopy and computer vision.

open access: yesPLoS ONE, 2014
We present a method for giant lipid vesicle shape analysis that combines manually guided large-scale video microscopy and computer vision algorithms to enable analyzing vesicle populations.
Jernej Zupanc   +6 more
doaj   +1 more source

A Note on Vestigial Osmotic Pressure

open access: yesMembranes, 2023
Recent experiments have indicated that at least a part of the osmotic pressure across the giant unilamellar vesicle (GUV) membrane was balanced by the rapid formation of the monodisperse daughter vesicles inside the GUVs through an endocytosis-like ...
Hao Wu   +2 more
doaj   +1 more source

Vesicle Clustering in a Living Synapse Depends on a Synapsin Region that Mediates Phase Separation

open access: yesCell Reports, 2020
Summary: Liquid-liquid phase separation is an increasingly recognized mechanism for compartmentalization in cells. Recent in vitro studies suggest that this organizational principle may apply to synaptic vesicle clusters. Here we test this possibility by
Arndt Pechstein   +8 more
doaj   +1 more source

17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells

open access: yesOncoTarget, 2014
A significant proportion of the genes regulated by 17-beta-estradiol (E2) via estrogen receptor alpha (ERα) have roles in vesicle trafficking in breast cancer.
P. K. Wright   +8 more
semanticscholar   +1 more source

Driven Engulfment of Janus Particles by Giant Vesicles in and out of Thermal Equilibrium

open access: yesNanomaterials, 2022
The interaction between Janus colloids and giant lipid vesicles was experimentally investigated to elucidate the dynamics and mechanisms related to microparticle engulfment by lipid vesicles.
Vaibhav Sharma   +2 more
doaj   +1 more source

One-Pot Assembly of Complex Giant Unilamellar Vesicle-Based Synthetic Cells

open access: yesACS Synthetic Biology, 2019
Here, we introduce a one-pot method for the bottom-up assembly of complex single- and multicompartment synthetic cells. Cellular components are enclosed within giant unilamellar vesicles (GUVs), produced at the milliliter scale directly from small ...
Kerstin Göpfrich   +6 more
semanticscholar   +1 more source

Application of a Novel Near Infrared-Fluorescence Giant Vesicle-and Polymerasome-based Tissue Marker for Endoscopic and Laparoscopic Navigation

open access: yesAnalytical Sciences, 2014
In this study, we describe the development of a novel tissue marker that can be injected from within the digestive tract by using an endoscopic instrument, and visualized using near-infrared (NIR) fluorescence imaging.
Hirosuke Hatayama   +4 more
semanticscholar   +1 more source

Large and Giant Unilamellar Vesicle(s) Obtained by Self-Assembly of Poly(dimethylsiloxane)-b-poly(ethylene oxide) Diblock Copolymers, Membrane Properties and Preliminary Investigation of Their Ability to Form Hybrid Polymer/Lipid Vesicles

open access: yesPolymers, 2019
In the emerging field of hybrid polymer/lipid vesicles, relatively few copolymers have been evaluated regarding their ability to form these structures and the resulting membrane properties have been scarcely studied.
M. Fauquignon   +5 more
semanticscholar   +1 more source

Osmotic Shrinkage of Giant Egg-Lecithin Vesicles [PDF]

open access: yesBiophysical Journal, 1980
Osmotic shrinkage of giant egg-lecithin vesicles was observed by phase-contrast microscopy. The vesicles remained or became spherical when shrinking. Small and thick-walled vesicles formed visible fingers attached to the sphere. The water permeability of the single bilayer was found to be 41 micrometers/s.
Boroske, E.   +2 more
openaire   +3 more sources

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