Results 71 to 80 of about 579,153 (203)

A distinct giant coat protein complex II vesicle population in Arabidopsis thaliana

open access: yesNature Plants, 2021
Baiying Li   +12 more
semanticscholar   +1 more source

In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension

open access: yesiScience, 2019
Summary: Artificial cells can shed new light on the molecular basis for life and hold potential for new chemical technologies. Inspired by how nature dynamically regulates its membrane compartments, we aim to repurpose the endosomal sorting complex ...
Andrew Booth   +3 more
doaj   +1 more source

Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol

open access: yesMolecules, 2015
In the present investigation we studied the molecular mechanisms of the monodesmosidic saponin digitonin on natural and artificial membranes. We measured the hemolytic activity of digitonin on red blood cells (RBCs). Also different lipid membrane models (
Ikhwan Resmala Sudji   +4 more
doaj   +1 more source

Optimization of the Inverted Emulsion Method for High‐Yield Production of Biomimetic Giant Unilamellar Vesicles

open access: yesChemBioChem, 2019
In the field of bottom‐up synthetic biology, lipid vesicles provide an important role in the construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's similarity to natural biomembranes, have been widely used as ...
Akanksha Moga   +3 more
semanticscholar   +1 more source

Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changing lipid composition determined by fluorescence measurements

open access: yesScientific Reports
To develop artificial cell models that mimic living cells, cell-sized lipid vesicles encapsulating cell-free protein synthesis (CFPS) systems are useful for protein expressions or artificial gene circuits for vesicle–vesicle communications.
Akari Miwa   +6 more
doaj   +1 more source

Curvature‐Assisted Vesicle Explosion Under Light‐Induced Asymmetric Oxidation

open access: yesAdvanced Science
Exposure of cell membranes to reactive oxygen species can cause oxidation of membrane lipids. Oxidized lipids undergo drastic conformational changes, compromising the mechanical integrity of the membrane and causing cell death.
Vinit Kumar Malik, On Shun Pak, Jie Feng
doaj   +1 more source

Reconstitution of circadian clock in synthetic cells reveals principles of timekeeping

open access: yesNature Communications
The cyanobacterial circadian clock maintains remarkable precision and synchrony, even in cells with femtoliter volumes. Here, we reconstitute the KaiABC post-translational oscillator (PTO) in giant unilamellar vesicles (GUVs) to investigate underlying ...
Alexander Zhan Tu Li   +2 more
doaj   +1 more source

Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles

open access: yesScientific Reports
Membrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun   +3 more
doaj   +1 more source

Genomic and structural insights into Jyvaskylavirus, the first giant virus isolated from Finland

open access: yeseLife
Giant viruses of protists are a diverse and likely ubiquitous group of organisms. Here, we describe Jyvaskylavirus, the first giant virus isolated from Finland.
Gabriel Magno de Freitas Almeida   +9 more
doaj   +1 more source

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