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Calorimetric Glucose Determination by Glucose Oxidase —Methylene Blue

Acta Biotechnologica, 1982
AbstractThe reaction between glucose and methylene blue, catalyzed by glucose oxidase (GOD)was analysed calorimetrically.The amount of heat produced under saturating methylene blue concentrations ( > 10−2 mol/1)was measured with glucose concentration and time as parameters (kinetic procedure) Kinetic constants (pseudo one substrate kinetics) were ...
D. Kirstein, L. Schilder, E. Kahrig
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The characterization of liposomal glucose oxidase electrodes for the measurement of glucose

Biosensors and Bioelectronics, 1995
Many biosensors have been described for the measurement of glucose in order to monitor diabetic patients. Glucose oxidase has been used commonly in the construction of glucose sensors but the performance of this enzyme is limited by enzyme saturation kinetics, which restrict the measurement of clinically relevant glucose concentrations (0 to 25 mM ...
M A, Taylor   +3 more
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Glucose oxidase?magnetite nanoparticle bioconjugate for glucose sensing

Analytical and Bioanalytical Chemistry, 2004
Immobilization of bioactive molecules on the surface of magnetic nanoparticles is of great interest, because the magnetic properties of these bioconjugates promise to greatly improve the delivery and recovery of biomolecules in biomedical applications.
Liane M, Rossi   +2 more
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Notes on the Glucose Oxidase Method

Scandinavian Journal of Clinical and Laboratory Investigation, 1967
This is a report of some methodological studies on the enzymatic determination of blood sugar, in particular concerning the composition of the enzyme mixture, the non-specific oxidation, the precipitation media, the effect of added heparin, fluoride, and benzoic acid, and the glutathione problem.
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A polarographic method for the rapid determination of glucose with glucose oxidase

Analytical Biochemistry, 1970
A rapid polarographic technique for the determination of glucose with a Clark-type O2 electrode and glucose oxidase is described. The method is sensitive and accommodates a greater range of glucose concentrations than the conventional spectrophotometric glucose oxidase procedure.
M, Jemmali, R, Rodriguez-Kabana
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Glucose oxidase immobilized electrode for potentiometric estimation of glucose

Biosensors and Bioelectronics, 1991
Glucose oxidase has been immobilized onto a thin platinum strip, by co-crosslinking with bovine serum albumin and glutaraldehyde. The retention of redox characteristics of glucose oxidase has been verified by cyclic voltammetry. The activity of the immobilized enzyme reduces to a quarter of its value when the enzyme is in solution but improves when ...
S, Alva   +3 more
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Glucose Biosensor Using Glucose Oxidase Immobilized in Polyaniline

Applied Biochemistry and Biotechnology, 1992
A biosensor for glucose utilizing kinetics of glucose oxidase (EC 1.1.3.4.) was developed. The enzyme was immobilized on polyaniline by covalent bonding, using glutaraldehyde as a bifunctional agent. The system showed a linear response up to 2.2 mM of glucose with a response time of 2.5-4.0 min. In addition, the immobilized enzyme had a higher activity
A H, Parente   +5 more
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Glucose Oxidase Multilayer Modified Microcantilevers for Glucose Measurement

Analytical Chemistry, 2005
We report a novel enzyme-based microcantilever sensor by using layer-by-layer nanoassembly modification. A glucose oxidase (GOx) functionalized microcantilever underwent bending when it was exposed to glucose solutions. The magnitudes of bending were proportional to the concentrations of glucose.
Xiaodong, Yan   +2 more
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Interference in glucose oxidase-peroxidase blood glucose methods

Clinica Chimica Acta, 1972
Abstract Interference by certain compounds with the glucose oxidase-peroxidase system used in blood glucose estimations appears to be a function of the oxygen affinity of the oxygen acceptor chromogen or the oxidative coupling system. It has been shown 1 that tolazamide interferes seriously with the Boehringer GOD-PERID blood glucose method.
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