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Effects of glucose oxidase in mice
Biochemical Pharmacology, 1964Abstract The effects of glucose oxidase, which oxidizes glucose and produces H2O2, were studied in mice after intraperitoneal injection of supralethal doses of the enzyme. Such injections cause the appearance of hematin pigment in the plasma, up to 20 per cent of this pigment being methemoglobin.
R N, FEINSTEIN, M, COULON, J E, SEAHOLM
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Some studies of glucose oxidase
Archives of Biochemistry and Biophysics, 1961Abstract A commercial preparation of glucose oxidase is shown to oxidize d -glucose, d -galactose, d -mannose, and d -xylose at relative rates which are pH dependent. The product of these reactions is the corresponding glyconic acid which in each case is identified as the crystalline γ-lactone and amide.
T T, HLAING, J P, HUMMEL, R, MONTGOMERY
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Glucose oxidase of Phanerochaete chrysosporium
1988Publisher Summary Glucose oxidase catalyzes the oxidation of D-glucose to δ-D-gluconolactone and H 2 O 2 in the presence of molecular oxygen. In a subsequent step δ -D-gluconolactone is nonenzymatically hydrolyzed to D-gluconic acid. This enzyme has been demonstrated in various Aspergillus and Penicillium species and has recently been purified ...
R L, Kelley, C A, Reddy
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Studies on conditions for the polarographic determination of glucose with glucose oxidase
Clinica Chimica Acta, 1972Abstract Results are presented on the effect of several variables on a method for the determination of glucose using a polarograph with a Clark-type O 2 electrode and glucose oxidase. Glucose concentration [ G ] was proportional to the rate of O 2 consumption |− Δ O 2 / Δt | in the 5–800 μg/ml range.
M, Jemmali, R, Rodríguez-Kábana
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Calorimetric Glucose Determination by Glucose Oxidase —Methylene Blue
Acta Biotechnologica, 1982AbstractThe reaction between glucose and methylene blue, catalyzed by glucose oxidase (GOD)was analysed calorimetrically.The amount of heat produced under saturating methylene blue concentrations ( > 10−2 mol/1)was measured with glucose concentration and time as parameters (kinetic procedure) Kinetic constants (pseudo one substrate kinetics) were ...
D. Kirstein, L. Schilder, E. Kahrig
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The characterization of liposomal glucose oxidase electrodes for the measurement of glucose
Biosensors and Bioelectronics, 1995Many biosensors have been described for the measurement of glucose in order to monitor diabetic patients. Glucose oxidase has been used commonly in the construction of glucose sensors but the performance of this enzyme is limited by enzyme saturation kinetics, which restrict the measurement of clinically relevant glucose concentrations (0 to 25 mM ...
M A, Taylor +3 more
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Glucose oxidase?magnetite nanoparticle bioconjugate for glucose sensing
Analytical and Bioanalytical Chemistry, 2004Immobilization of bioactive molecules on the surface of magnetic nanoparticles is of great interest, because the magnetic properties of these bioconjugates promise to greatly improve the delivery and recovery of biomolecules in biomedical applications.
Liane M, Rossi +2 more
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Notes on the Glucose Oxidase Method
Scandinavian Journal of Clinical and Laboratory Investigation, 1967This is a report of some methodological studies on the enzymatic determination of blood sugar, in particular concerning the composition of the enzyme mixture, the non-specific oxidation, the precipitation media, the effect of added heparin, fluoride, and benzoic acid, and the glutathione problem.
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A polarographic method for the rapid determination of glucose with glucose oxidase
Analytical Biochemistry, 1970A rapid polarographic technique for the determination of glucose with a Clark-type O2 electrode and glucose oxidase is described. The method is sensitive and accommodates a greater range of glucose concentrations than the conventional spectrophotometric glucose oxidase procedure.
M, Jemmali, R, Rodriguez-Kabana
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Glucose oxidase immobilized electrode for potentiometric estimation of glucose
Biosensors and Bioelectronics, 1991Glucose oxidase has been immobilized onto a thin platinum strip, by co-crosslinking with bovine serum albumin and glutaraldehyde. The retention of redox characteristics of glucose oxidase has been verified by cyclic voltammetry. The activity of the immobilized enzyme reduces to a quarter of its value when the enzyme is in solution but improves when ...
S, Alva +3 more
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