Results 111 to 120 of about 30,715 (166)
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Effect of phosphonic analogues of glutamic acid on glutamate decarboxylase
Experientia, 1985Among the phosphonic analogues of glutamic acid, only 4-amino-4-phosphono butyric acid, the compound which shows the highest affinity for pyridoxal phosphate, inhibits competitively both Escherichia coli and rat brain glutamate decarboxylases. Phosphinothricin, 2-amino-4-(methylphosphino)butyric acid, is a strong inhibitor of the mammalian enzyme.
A M, Lacoste +3 more
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Stereochemistry of reactions catalyzed by glutamate decarboxylase
Biochemistry, 1978When the decarboxylation of L-glutamic acid by the glutamate decarboxylase from Escherichia coli is carried out in D2O, the product gamma-aminobutyric acid contains a single deuterium atom. The stereochemistry of this material was established by conversion to levorotatory methyl 4-phthalimido [4(-2)H] butyrate.
H, Yamada, M H, O'Leary
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Borohydride reduction of l-glutamate decarboxylase
Archives of Biochemistry and Biophysics, 1965Abstract l -Glutamate decarboxylase was purified from a sucrose-negative strain of Escherichia coli . The specific activity of 60% pure enzyme was 19,550 μl/10 minutes/mg. Borohydride reduction at pH 4.8–5.2 resulted in (a) the disappearance of the peak at 415 mμ, and appearance of an inflection at 330 mμ; (b) loss of 97% of the activity; (c ...
J A, ANDERSON, H F, CHANG
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1979
Much progress has been made in recent years regarding enzymological aspects of mammalian brain GAD, such as its purification and characterization, but some uncertainty still remains concerning its molecular weight and forms, and its subunit structure.
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Much progress has been made in recent years regarding enzymological aspects of mammalian brain GAD, such as its purification and characterization, but some uncertainty still remains concerning its molecular weight and forms, and its subunit structure.
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Inhibitors of crayfish glutamic acid decarboxylase
Neurochemical Research, 1984Crayfish glutamic acid decarboxylase (GAD), like the homologous enzymes from other species, is inhibited by carbonyl-trapping agents (e.g. aminooxyacetic acid; AOAA) and sulfhydryl reagents (e.g. 5,5'-dithiobis-(2-nitrobenzoic acid); DTNB). It also is inhibited by the product GABA, many anions (e.g. SCN- and Cl-), and some cations (e.g. Zn+2).
R M, Grossfeld, S W, Yancey, C F, Baxter
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Glutamic acid decarboxylase in Mycobacterium leprae
Archives of Microbiology, 1983Suspensions of Mycobacterium leprae purified from the organs (mostly spleen) of experimentally-infected armadillos (Dasypus novemcinctus, Linn) decarboxylated 1-(14C) glutamic acid liberating 14CO2. The reaction was pyridoxal phosphate-dependent and was inhibited by hydroxylamine, suggesting that it is a true amino acid decarboxylase.
K, Prabhakaran +2 more
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Structure and function ofl-glutamate decarboxylase
Neurochemical Research, 1991Membrane bound L-glutamate decarboxylase (GAD) has been solubilized and partially purified from hog brain. The solubilized GAD appears to exist in two forms, alpha and beta, differing in their size and electrophoretic mobility. The alpha form has similar mobility as that of the soluble GAD in 7.5% and 5-25% gradient polyacrylamide gel electrophoresis ...
J Y, Wu +6 more
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Coenzyme binding site of glutamate decarboxylase
Biochemistry, 1980Dissociation constants have been measured for the binding of a variety of simple analogues of pyridoxal 5'-phosphate to apoglutamate decarboxylase. Compounds studied have a simple alkyl or aryl group and a negatively charged substituent (phosphate, phosphonate, phosphoramidate, sulfate, sulfonate, or carboxylate).
M H, O'Leary, S W, Koontz
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Plasma glutamate decarboxylase activity in neuropsychiatry
Psychiatry Research, 1982Plasma glutamate decarboxylase (GAD) activity was measured in patients with endogenous psychoses and neurologic diseases. Unmedicated schizophrenic patients showed no difference in plasma GAD levels compared to controls. Administration of neuroleptics together with anticholinergic agents increased plasma GAD activity in schizophrenic patients. Compared
H, Kaiya +3 more
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Radioimmunoassay for Glutamic Acid Decarboxylase-65
Diabetes Technology & Therapeutics, 1999Glutamic acid decarboxylase-65 (GAD65), the enzyme that catalyzes the formation of gamma-aminobutyric acid (GABA), is the major autoantigen in both type 1 (insulin-dependent) diabetes and stiff-man syndrome (SMS). The observation that GAD65 autoantibodies may be present for years before the clinical onset of diabetes raises the question of when GAD65 ...
W, Hao +6 more
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