Results 251 to 260 of about 53,702 (285)

Measurement of Glutathione and Glutathione Disulfide

Current Protocols in Toxicology, 1999
AbstractMeasurements of glutathione should include quantification of both the reduced and oxidized forms. HPLC‐based assays for glutathione and other cellular thiols and disulfides utilize a variety of detection methods, including ultraviolet, fluorescence, and electrochemical methods.
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Effect of the glutathione/glutathione disulfide redox couple on thiopurine methyltransferase

Biochemical Pharmacology, 2001
The susceptibility of recombinant human thiopurine methyltransferase (hTPMT) to thiol-disulfide exchange was investigated. The enzyme was incubated in buffers of the redox couple GSH and GSSG. The values of the chosen concentrations and concentration ratios of the redox couple equaled those expected to occur in vivo. Activity measurements of the enzyme
R A, Lysaa , D J, Warren, I, Sylte, J, Aarbakke   +3 more
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Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine

Analytical Biochemistry, 1980
Abstract The total glutathione content of biological samples is conveniently determined with an enzymatic recycling assay based on glutathione reductase ( F. Tietze, 1969 , Anal. Biochem. 27 , 502–522). In the original and several subsequent descriptions of this procedure, glutathione disulfide is selectively determined by assaying samples in which
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High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides

Analytical Biochemistry, 1980
A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20 related sulfur-containing amino acids or their derivatives has been described.
D J, Reed, J R, Babson, P W, Beatty, A E, Brodie, W W, Ellis, D W, Potter   +5 more
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Hypoxia increases plasma glutathione disulfide in rats

Lung, 1989
We tested the hypothesis that hypoxia causes cellular oxidative stress by measuring plasma concentrations of glutathione disulfide (GSSG) in rats exposed to acute and subacute hypoxia. In awake, unanesthetized, catheter-implanted rats, exposure to 8% O2 for 10 min caused pulmonary vasoconstriction and increased plasma GSSG. This increase in plasma GSSG
S W, Chang, T J, Stelzner, J V, Weil, N F, Voelkel   +3 more
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Sensitive enzymatic cycling assay for glutathione and glutathione disulfide content

Analytica Chimica Acta
It is crucial to develop reliable techniques for accurately detecting and measuring glutathione (GSH) and glutathione disulfide (GSSG) content in biological samples. However, there is currently not yet a full consensus on the best approach for preparing and analyzing samples to obtain accurate quantitative results.This study screened N-ethylmaleimide ...
Xinyue Lin, Yajun Chu, Qianhe Xu, Qiuying Nie, Cui Xu, Jianguo Fang, Junmin Zhang   +6 more
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Mechanisms of glutathione disulfide efflux from erythrocytes

Biochemical Pharmacology, 2012
Glutathione (GSH) plays numerous critical protective roles in the erythrocyte and GSH turnover is likely an important factor in regulating susceptibility to oxidative stress and toxins. Efflux of glutathione disulfide (GSSG) from erythrocytes is an important component in the regulation of GSH levels; however, little is known of the mechanisms involved.
Irina, Ellison, John P, Richie
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Coenzyme A Glutathione Disulfide

Circulation Research, 1995
Abstract The adrenal gland is involved in the regulation of vascular tone by secretion of vasoactive agents such as catecholamines, neuropeptide Y, or endogenous ouabain. A further potent vasoconstrictor is isolated from bovine adrenal glands and is identified by chromatography, mass spectrometry, UV spectroscopy, and enzymatic
Hartmut Schlüter, Michael Meissner, Marcus van der Giet, Martin Tepel, Jürgen Bachmann, Isolde Groß, Eckhard Nordhoff, Michael Karas, Claus Spieker, Herbert Witzel, Walter Zidek   +10 more
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Glutathione and glutathione disulfide – their biomedical and pharmaceutical applications

Medicinal Chemistry Research, 2023
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The Glutathione–Glutathione Disulfide System

1976
Nechama S. Kosower, Edward M. Kosower
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