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Mutants lacking glutelin subunits in rice: mapping and combination of mutated glutelin genes
Theoretical and Applied Genetics, 1997Nine mutant lines lacking glutelin subunits were selected from M2 seeds of about 10000 M1 plants mutagenized with gamma rays or EMS and from 1400 mutant lines selected originally for morphological characters. There were three types of mutants, one line lacking the largest subunit among four minor bands of glutelin acidic subunits (Type 1), five lines ...
S. Iida, M. Kusaba, T. Nishio
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Transgenic rice with reduced glutelin content by transformation with glutelin A antisense gene
Molecular Breeding, 2002Rice plants were genetically transformed with a glutelin A antisensegene construct that was characterized by eight tandem repeats of a ca.0.3 kb fragment at the 5′ end of full-length glutelin AcDNA.Two transgenic rice lines, H39-59 and H75-3, homozygous for the glutelin Aantisense gene were selected from T2 plants, and their T4generation was examined ...
Yoshiyuki Maruta +4 more
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A rice glutelin gene family — a major type of glutelin mRNAs can be divided into two classes
Molecular and General Genetics MGG, 1987Rice glutelin cDNA clones were isolated from a rice cDNA library constructed from immature endosperm. The isolated glutelin cDNA clones could be divided into two classes by restriction site polymorphism and sequence. They encoded polypeptides containing the same number of amino acids including a 24 amino acid signal peptide, a 282 amino acid acidic ...
Fumio Takaiwa +2 more
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Measurements on disulphide bonds in cereal glutelins
Journal of the Science of Food and Agriculture, 1971AbstractCereal glutelins were divided by dilute acetic acid extraction into‘soluble’and‘insoluble’fractions. The quantities dissolved in the cases of oats and maize were negligibly small. The S.S bonds of these fractions from wheat, rye, barley, oats and maize have been amperometrically titrated after reaction with sulphite in the absence and presence ...
D. G. Redman, J. A. D. Ewart
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Extraction and composition of rice endosperm glutelin
Phytochemistry, 1976Abstract “Crude” glutelin was prepared from milled rice (Oryza sativa) flour by sequential extraction of the albumin-globulin fraction with 0.5 M NaCl and prolamin with 70% ethanol-0.6% β-mercaptoethanol. The solvent, 0.5% sodium dodecyl sulphate (SDS)-0.6% β-mercaptoethanol, extracted 91% of the endosperm glutelin without gelatinizing starch ...
Bienvenido O. Juliano, Donald Boulter
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Fractionation and properties of alkylated-reduced corn glutelin proteins
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1971Abstract The disulfide bonds of glutelin protein from corn endosperm were cleaved to yield polypeptide chains which were subjected to physical and chemical investigations. After reduction with mercaptoethanol and alkylation with acrylonitrile of resulting sulfhydryls, about 87% of the protein was soluble in 6 M guanidine-HC1 and 47% in 0.3 M acetic ...
J W, Paulis, J S, Wall
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Further studies on SS bonds in cereal glutelins
Journal of the Science of Food and Agriculture, 1972AbstractFlours of wheat, rye, barley, oats, maize and sorghum have been extracted to remove albumins, globulins and prolamins. The SS bonds of the starch‐glutelin residues (4 to 8% protein) have been reduced with sulphite and titrated with phenyl mercury acetate (PMA) in the presence of 3 M‐guanidine hydrochloride at 37 °C and, in its absence, at 2 and
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Physicochemical properties of pronase‐treated rice glutelin
Journal of the American Oil Chemists' Society, 2001AbstractRice glutelin protein was extracted from defatted medium‐grain rice by alkali extraction followed by acid precipitation. Extracted glutelin was hydrolyzed with Pronase E, a bacterial protease, and the functional properties of hydrolysates were evaluated.
A. Anderson, N. Hettiarachchy, Z. Y. Ju
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