Results 51 to 60 of about 11,445 (270)

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source

Tissue-characteristic forms of glyceraldehyde 3-phosphate dehydrogenase [PDF]

Biochemical Journal, 1970
Glyceraldehyde 3-phosphate dehydrogenase exists in two different forms in various human tissue preparations. One of them is exhibited, after starch-gel electrophoresis, by a rapidly migrating or `fast' band and the other by a `slow' band. The proportion of the total activity in each of the two forms is characteristic of the type of tissue.
N, Ressler, S, Lee
openaire   +2 more sources

Characterization of three 2-hydroxy-acid dehydrogenases in the context of a biotechnological approach to short-circuit photorespiration [PDF]

, 2010
Photorespiration results from the incorporation of oxygen into ribulose-1,5-bisphosphate due to the failure of RuBisCO to properly discriminate between oxygen and carbon dioxide.
Engqvist, Martin
core  

A systems biology approach reveals major metabolic changes in the thermoacidophilic archaeon Sulfolobus solfataricus in response to the carbon source L-fucose versus D-glucose [PDF]

, 2016
Archaea are characterised by a complex metabolism with many unique enzymes that differ from their bacterial and eukaryotic counterparts. The thermoacidophilic archaeon Sulfolobus solfataricus is known for its metabolic versatility and is able to utilize ...
Albaum, S.P., Albers, S.V., Albersmeier, A., Brasen, C., Fafenrot, K., Hoffmann, L., Kalinowski, J., Kouril, T., Meyer, B., Muller, K.B., Neumann-Schaal, M., Pham, T., Schmidt-Hohagen, K., Schomburg, D., Shen, L., Siebers, B., Stark, H., Wolf, J., Wright, P.C.   +18 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli [PDF]

Journal of Bacteriology, 1975
We describe glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. The gene (gap) is at approximately 34 min, with the transductional order gap-fadD-eda. One gap mutant is temperature sensitive and has a heat-labile enzyme. Another is amber.
J D, Hillman, D G, Fraenkel
openaire   +2 more sources

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Lipocalin‐2 activates hepatic stellate cells and promotes nonalcoholic steatohepatitis in high‐fat diet–fed Ob/Ob mice

Hepatology, EarlyView., 2022
Graphical summary of obesity‐induced NASH progression by LCN2 targeted to HSC activation. Abstract Background and Aims In obesity and type 2 diabetes mellitus, leptin promotes insulin resistance and contributes to the progression of NASH via activation of hepatic stellate cells (HSCs).
Kyung Eun Kim, Jaewoong Lee, Hyun Joo Shin, Eun Ae Jeong, Hye Min Jang, Yu Jeong Ahn, Hyeong Seok An, Jong Youl Lee, Meong Cheol Shin, Soo Kyoung Kim, Won Gi Yoo, Won Ho Kim, Gu Seob Roh   +12 more
wiley   +1 more source

A synthetic biochemistry platform for cell free production of monoterpenes from glucose. [PDF]

, 2017
Cell-free systems designed to perform complex chemical conversions of biomass to biofuels or commodity chemicals are emerging as promising alternatives to the metabolic engineering of living cells.
Bowie, James U, Korman, Tyler P, Opgenorth, Paul H   +2 more
core   +2 more sources