Glycine metabolism inCandida albicans: characterization of the serine hydroxymethyltransferase (SHM1, SHM2) and threonine aldolase (GLY1) genes [PDF]
Yeast, 2000 Genes encoding the mitochondrial (SHM1) and cytosolic (SHM2) serine hydroxymethyltransferases, and the L-threonine aldolase gene (GLY1) from Candida albicans were cloned and sequenced. All three genes are involved in glycine metabolism. The C. albicans Shm1 protein is 82% identical to that from Saccharomyces cerevisiae and 56% identical to that from ...
J B, McNeil +7 more
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Interaction between glycine decarboxylase, serine hydroxymethyltransferase and tetrahydrofolate polyglutamates in pea leaf mitochondria [PDF]
Biochemical Journal, 1994 The aim of the present work was to further determine how the T-protein of the glycine-cleavage system and serine hydroxy-methyltransferase (SHMT), two folate-dependent enzymes from pea leaf mitochondria, interact through a common pool of tetrahydrofolate polyglutamates (H4PteGlun).
F, Rebeille, M, Neuburger, R, Douce
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Serine Hydroxymethyltransferase and Glycine Cleavage Enzyme from the Cyanogenic Bacterium Chromobacterium violaceum [PDF]
Microbiology, 1984 Chromobacterium violaceum forms cyanide as a secondary metabolite with glycine as the precursor and methionine as a stimulator. As the metabolism of glycine and methionine are interrelated by the provision of carbon-one units for the tetrahydrofolate pool by the activities of serine hydroxymethyltransferase (SHMT) and glycine cleavage enzyme (GCE), we ...
S. P. SHIRAZI-BEECHEY, C. J. KNOWLES
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Low Serine Hydroxymethyltransferase Activity in the Human Placenta Has Important Implications for Fetal Glycine Supply [PDF]
The Journal of Clinical Endocrinology & Metabolism, 2005 Glycine is essential for fetal development, but in both sheep and human pregnancy, little is transported directly from the mother to the fetus, indicating that fetal glycine is derived from other sources. In the sheep, placental conversion of maternal serine by serine hydroxymethyltransferase (SHMT) provides almost all the glycine transported to the ...
Lewis, Rohan M. +4 more
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Resolution and characterization of the glycine-cleavage reaction in pea leaf mitochondria. Properties of the forward reaction catalysed by glycine decarboxylase and serine hydroxymethyltransferase [PDF]
Biochemical Journal, 1988 High-molecular-mass proteins from pea (Pisum sativum) mitochondrial matrix retained on an XM-300 Diaflo membrane (‘matrix extract’) exhibited high rates of glycine oxidation in the presence of NAD+ and tetrahydropteroyl-L-glutamic acid (H4 folate) as long as the medium exhibited a low ionic strength.
J, Bourguignon, M, Neuburger, R, Douce
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Effects of tetrahydrofolate polyglutamates on the kinetic parameters of serine hydroxymethyltransferase and glycine decarboxylase from pea leaf mitochondria [PDF]
Biochemical Journal, 1993 Plant tissues contain highly conjugated forms of folate. Despite this, the ability of plant folate-dependent enzymes to utilize tetrahydrofolate polyglutamates has not been examined in detail. In leaf mitochondria, the glycine-cleavage system and serine hydroxymethyltransferase, present in large amounts in the matrix space and involved in the ...
V, Besson +4 more
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Decarboxylation of glycine by serine hydroxymethyltransferase in the presence of lipoic acid.
Journal of Biological Chemistry, 1983 Serine hydroxymethyltransferase and the glycine cleavage system are both present in liver mitochondria and both bind glycine to form a pyridoxal 5'-phosphate carbanionic quinoid species. Lipoic acid has been shown to have the ability to intercept the carbanionic intermediate formed from the binary complex of serine hydroxymethyltransferase and glycine ...
L R, Zieske, L, Davis
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Effects of Sulfanilamide and Methotrexate on 13C Fluxes through the Glycine Decarboxylase/Serine Hydroxymethyltransferase Enzyme System in Arabidopsis1 [PDF]
Plant Physiology, 1998 Abstract In C3 plants large amounts of photorespiratory glycine (Gly) are converted to serine by the tetrahydrofolate (THF)-dependent activities of the Gly decarboxylase complex (GDC) and serine hydroxymethyltransferase (SHMT). Using 13C nuclear magnetic resonance, we monitored the flux of carbon through the GDC/SHMT enzyme system in ...
Vikram Prabhu +4 more
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Regulation of Glycine Decarboxylase and l-Serine Hydroxymethyltransferase Activities by Glyoxylate in Tobacco Leaf Mitochondrial Preparations [PDF]
Plant Physiology, 1982 Glyoxylate at a concentration of 10 millimolar caused 50% inhibition of decarboxylation of 20 millimolar [1-(14)C]glycine and accompanying synthesis of serine in a mitochondria-enriched preparation from tobacco (Nicotiana tabacum var. John Williams Broadleaf) leaves. None of the other compounds tested including formate, acetate, oxalate, aspartate, and
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13C Nuclear Magnetic Resonance Detection of Interactions of Serine Hydroxymethyltransferase with C1-Tetrahydrofolate Synthase and Glycine Decarboxylase Complex Activities in Arabidopsis [PDF]
Plant Physiology, 1996 Abstract In C3 plants, serine synthesis is associated with photorespiratory glycine metabolism involving the tetrahydrofolate (THF)-dependent activities of the glycine decarboxylase complex (GDC) and serine hydroxymethyl transferase (SHMT). Alternatively, THF-dependent serine synthesis can occur via the C1-THF synthase/SHMT pathway.
V, Prabhu +3 more
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