Results 61 to 70 of about 23,307 (259)

High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery [PDF]

open access: yes, 2011
Kim T-W, Chokhawala HA, Hess M, et al. High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery. Angewandte Chemie.
Craig M. Dana   +17 more
core   +1 more source

Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ

open access: yesPlants, 2014
Techniques for in situ localization of gene products provide indispensable information for understanding biological function. In the case of enzymes, biological function is directly related to activity, and therefore, knowledge of activity patterns is ...
Alicja Banasiak   +3 more
doaj   +1 more source

A Metagenome‐Assembled Genome Catalog From the Global Ruminant Microbiomes

open access: yesAnimal Research and One Health, EarlyView.
The Ruminant Gastrointestinal MAG Catalog (RGMC) is a comprehensive global resource offering 40,812 strain‐level genomes across 53 bacterial and 4 archaeal classes. It greatly surpasses prior efforts in scale and diversity, serving as an essential foundation for research in ruminant nutrition, microbial function, and methane mitigation.
Shizhe Zhang   +8 more
wiley   +1 more source

Purification of glycoside hydrolases from Bacteroides fragilis [PDF]

open access: yesApplied and Environmental Microbiology, 1980
Six glycoside hydrolases in the culture medium of Bacteroides fragilis--alpha-glucosidase, beta-glucosidase, alpha-galactosidase, beta-galactosidase, beta-N-acetylglucosaminidase, and alpha-L-fucosidase-were systematically purified by ammonium sulfate precipitation, gel filtration chromatography, and density gradient isoelectric focusing.
J O, Berg, L, Lindqvist, C E, Nord
openaire   +2 more sources

Polycationic Peptide Engineering of Phage Endolysins Expands Host Range and Enhances Antibacterial and Antibiofilm Activities Against Bacillus Species

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT The exponential rise in antimicrobial resistance has highlighted the urgent need for the development of alternative antibacterial strategies, including phage‐derived and engineered protein agents such as endolysins and artilysins. In this study, we report the rational design, expression, and functional characterization of novel artilysins ...
Farhat Ansari, Tarushi, Vandan Nagar
wiley   +1 more source

Selective Isolation of Bifidobacterium From Human Faeces Using Pangenomics, Metagenomics, and Enzymology

open access: yesFrontiers in Microbiology, 2021
Bifidobacterium, an important genus for human health, is difficult to isolate. We applied metagenomics, pangenomics, and enzymology to determine the dominant glycoside hydrolase (GH) families of Bifidobacterium and designed selective medium for ...
Shuanghong Yang   +12 more
doaj   +1 more source

Glycoside Hydrolases for Extraction and Modification of Polyphenolic Antioxidants [PDF]

open access: yes, 2013
Antioxidants are important molecules that are widely used by humans, both as dietary supplements and as additives to different types of products. In this chapter, we review how flavonoids, a class of polyphenolic antioxidants that are often found in glycosylated forms in many natural resources, can be extracted and modified using glycoside hydrolases ...
Gulshan Kazi, Zubaida   +4 more
openaire   +2 more sources

Synthesis and Glycosidase Inhibition Studies of Novel Exoglycals Targeting GH3 Family Enzymes: Insights from Comparative Analysis with Macrolide Antibiotics

open access: yesChemistry – A European Journal, EarlyView.
A series of structurally diverse exoglycals was synthesized using a modified Julia‐olefination approach from sugar‐derived precursors. These glycosidase transition‐state mimics were further diversified via CuAAC chemistry to yield triazole‐conjugated analogues.
Elisa Ospanow   +2 more
wiley   +1 more source

Stage‐Dependent Metabolic Reprogramming Drives a Self‐Organizing Microbial Network During Shanxi Aged Vinegar

open access: yesFood Bioengineering, EarlyView.
Metabolic shifts from growth to stress adaptation in Shanxi aged vinegar fermentation link flavor maturation and microbial cooperation, and Lactobacillus and Acetobacter form a self‐organizing metabolic network through division of labor. ABSTRACT Acetic acid fermentation (AAF) is a vital stage in the production of Shanxi aged vinegar (SAV), during ...
Yanfang Wu   +6 more
wiley   +1 more source

Modular structures of prominent glycoside hydrolases. [PDF]

open access: yes, 2013
These are compared with the predicted enzymes showing the highest % similarity (Blast) from R. flavefaciens FD-1 or R. albus: (A) glycoside hydrolase family 48; (B) glycoside hydrolase family 9; (C) glycoside hydrolase family 26.
Raphael Lamed (47762)   +12 more
core   +1 more source

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