Results 41 to 50 of about 32,747,610 (220)

Unraveling the subtleties of β-(1→3)-glucan phosphorylase specificity in the GH94, GH149, and GH161 glycoside hydrolase families [PDF]

Journal of Biological Chemistry, 2019
Glycoside phosphorylases (GPs) catalyze the phosphorolysis of glycans into the corresponding sugar 1-phosphates and shortened glycan chains. Given the diversity of natural β-(1→3)-glucans and their wide range of biotechnological applications, the identification of enzymatic tools that can act on β-(1→3)-glucooligosaccharides is an attractive area of ...
Sakonwan Kuhaudomlarp, Giulia Pergolizzi, Nicola J. Patron, Bernard Henrissat, Robert A. Field   +4 more
openaire   +5 more sources

Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2

AMB Express, 2019
The products of bacterial β-glucosidases with favorable cold-adapted properties have industrial applications. A psychrophilic β-glucosidase gene named bglG from subtropical soil microorganism Exiguobacterium sp.
Bangqiao Yin, Hengsen Gu, Xueyan Mo, Yue Xu, Bing Yan, Quanwen Li, Qian Ou, Bo Wu, Chen Guo, Chengjian Jiang   +9 more
doaj   +1 more source

The plant Nudix hydrolase family. [PDF]

, 2008
Nudix hydrolases are a family of proteins defined by a conserved amino-acid sequence GX(5)-EX(7)REUXEEXGU, where U is a hydrophobic residue. These enzymes are widely distributed among all classes of organisms and catalyze, with varying degrees of ...
Kraszewska, Elzbieta
core   +2 more sources

The structural and functional contributions of β-glucosidase-producing microbial communities to cellulose degradation in composting

Biotechnology for Biofuels, 2018
Background Compost habitats sustain a vast ensemble of microbes that engender the degradation of cellulose, which is an important part of global carbon cycle. β-Glucosidase is the rate-limiting enzyme of degradation of cellulose.
Xiangyun Zang, Meiting Liu, Yihong Fan, Jie Xu, Xiuhong Xu, Hongtao Li   +5 more
doaj   +1 more source

Discovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose

Scientific Reports, 2022
Glycoside phosphorylases (GPs), which catalyze the reversible phosphorolysis of glycosides, are promising enzymes for the efficient production of glycosides.
Wataru Saburi, Takanori Nihira, Hiroyuki Nakai, Motomitsu Kitaoka, Haruhide Mori   +4 more
doaj   +1 more source

Kinetic characterization of a glycoside hydrolase family 44 xyloglucanase/endoglucanase from Ruminococcus flavefaciens FD-1

Enzyme and Microbial Technology, 2011
Two forms of Ruminococcus flavefaciens FD-1 endoglucanase B, a member of glycoside hydrolase family 44, one with only a catalytic domain and the other with a catalytic domain and a carbohydrate binding domain (CBM), were produced. Both forms hydrolyzed cellotetraose, cellopentaose, cellohexaose, carboxymethylcellulose (CMC), birchwood and larchwood ...
Warner, Christopher, Go, Rica, García-Salinas, Carolina, Ford, Clark, Reilly, Peter   +4 more
openaire   +4 more sources

A novel accessory protein ArCel5 from cellulose-gelatinizing fungus Arthrobotrys sp. CX1

Bioresources and Bioprocessing, 2022
Improved understanding of cellulose swelling mechanism is beneficial for increasing the hydrolysis efficiency of cellulosic substrates. Here, we report a family 5 glycoside hydrolase ArCel5 isolated from the cellulose-gelatinizing fungus Arthrobotrys sp.
Yue Yuan, Chunshu Chen, Xueyan Wang, Shaonian Shen, Xiaoyu Guo, Xiaoyi Chen, Fan Yang, Xianzhen Li   +7 more
doaj   +1 more source

A novel α-L-arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum.

PLoS ONE, 2013
The study describes a comparative analysis of biochemical, structural and functional properties of two recombinant derivatives from Clostridium thermocellum ATCC 27405 belonging to family 43 glycoside hydrolase. The family 43 glycoside hydrolase encoding
Shadab Ahmed, Ana Sofia Luis, Joana L A Bras, Arabinda Ghosh, Saurabh Gautam, Munishwar N Gupta, Carlos M G A Fontes, Arun Goyal   +7 more
doaj   +1 more source

Analysis of Amyloid Fibrillation of Two Family 1 Glycoside Hydrolases

International Journal of Molecular Sciences
The formation and analysis of amyloid fibers by two β-glucosidases, BglA and BglB, belonging to the GH1 enzyme family, are reported. Both proteins have the (β/α)8 TIM-barrel fold, which is characteristic of this family and is also the most common protein structure. BglA is an octamer, whereas BglB is a monomer.
Miguel A. Rodríguez-López, José María Coll-Marqués, David Talens-Perales, Julia Marín-Navarro, Julio Polaina, Edgar Vázquez-Contreras   +5 more
openaire   +3 more sources

Structural Insights into the Carbohydrate Binding Ability of an α-(1→2) Branching Sucrase from Glycoside Hydrolase Family 70 [PDF]

Journal of Biological Chemistry, 2016
The α-(1→2) branching sucrase ΔN123-GBD-CD2 is a transglucosylase belonging to glycoside hydrolase family 70 (GH70) that catalyzes the transfer ofd-glucosyl units from sucroseto dextrans or gluco-oligosaccharides via the formation of α-(1→2) glucosidic linkages.
Brison, Yoann, Malbert, Yannick, Czaplicki, Georges, Mourey, Lionel, Remaud-Simeon, Magali, Tranier, Samuel   +5 more
openaire   +4 more sources