Results 51 to 60 of about 32,747,610 (220)

Combinatorial engineering of dextransucrase specificity. [PDF]

PLoS ONE, 2013
We used combinatorial engineering to investigate the relationships between structure and linkage specificity of the dextransucrase DSR-S from Leuconostoc mesenteroides NRRL B-512F, and to generate variants with altered specificity.
Romain Irague, Laurence Tarquis, Isabelle André, Claire Moulis, Sandrine Morel, Pierre Monsan, Gabrielle Potocki-Véronèse, Magali Remaud-Siméon   +7 more
doaj   +1 more source

Substrate specificity of glycoside hydrolase family 1 β-glucosidase AtBGlu42 fromArabidopsis thalianaand its molecular mechanism

Bioscience, Biotechnology, and Biochemistry, 2021
ABSTRACTPlants possess many glycoside hydrolase family 1 (GH1) β-glucosidases, which physiologically function in cell wall metabolism and activation of bioactive substances, but most remain uncharacterized. One GH1 isoenzyme AtBGlu42 in Arabidopsis thaliana has been identified to hydrolyze scopolin using the gene deficient plants, but no enzymatic ...
Shu Horikoshi, Wataru Saburi, Jian Yu, Hideyuki Matsuura, James R Ketudat Cairns, Min Yao, Haruhide Mori   +6 more
openaire   +3 more sources

Analysis of two single trait loci affecting flavonol glycoside accumulations in Arabidopsis thaliana natural variations [PDF]

, 2007
Ishihara H. Analysis of two single trait loci affecting flavonol glycoside accumulations in Arabidopsis thaliana natural variations. Bielefeld (Germany): Bielefeld University; 2007.Various plant secondary metabolites, including flavonoids, are involved ...
Ishihara, Hirofumi
core  

Glycoside Hydrolase Family 63 nucleotide sequences

, 2017
Fasta file with nucleotide sequences of Glycoside Hydrolase Family 63 protein from different fungal strains. In the file the following information of each sequence can be found: -organism -mol_type= type of molecule -tissue_type=origin of the fungal ...
Buée, Marc, Pérez-Izquierdo, Leticia, Maurice, Jean-Paul, Morin, Emmanuelle, Martin, Francis, Rincón, Ana   +5 more
core   +1 more source

Global characterization of GH3 family glycoside hydrolase genes in Fusarium verticillioides and functional analysis of FvGH3-6

Frontiers in Microbiology
To clarify the roles of glycoside hydrolase 3 (GH3) family genes in the growth, development, and pathogenicity of Fusarium verticillioides, GH3 family genes were identified in the genome by bioinformatics software, and their expression levels in the ...
Xiaojie Zhang, Xiaojie Zhang, Pengliang Duan, Pengliang Duan, Shaoqi Shi, Shaoqi Shi, Manli Sun, Manli Sun, Ning Liu, Ning Liu, Zhiyan Cao, Zhiyan Cao, Jingao Dong, Jingao Dong   +13 more
doaj   +1 more source

Oligosaccharides With Defined Glycosidic Bonds Shape Gut Microbial Succession and Metabolism Via Bond‐Specific Microbial Responders

Advanced Science, EarlyView.
Oligosaccharides with defined glycosidic bonds drive divergent gut microbial succession and shape unique metabolic profiles. Absolute time‐series profiling with Bayesian generalized Lotka–Volterra modeling identifies bond‐specific microbial responders, while monoculture validation and metaproteomics reveal linkage‐matched transport and cleavage modules.
Xiaoxuan Lu, Jiaqi Zou, Geng Han, Mengyao Zhao, Ting Luo, Xiangru Feng, Liangliang Zhu, Yijia Chen, Xiaoguo Ji, Jiayang Jin, Liming Zhao   +10 more
wiley   +1 more source

A new group of glycoside hydrolase family 13 α-amylases with an aberrant catalytic triad [PDF]

, 2017
α-Amylases are glycoside hydrolase enzymes that act on the α(1→4) glycosidic linkages in glycogen, starch, and related α-glucans, and are ubiquitously present in Nature. Most α-amylases have been classified in glycoside hydrolase family 13 with a typical
null Ihsanawati, Ocky K. Radjasa, Dessy Natalia, Ihsanawati,, Fean D. Sarian, Zeily Nurachman, Radjasa, Ocky K, Marc J. E. C. van der Maarel, Sarian, Fean D, Dijkhuizen, Lubbert, Janeček, Štefan, Tjaard Pijning, Dijkhuizen, Lubbert; id_orcid, Lubbert Dijkhuizen, Pijning, Tjaard; id_orcid, van der Maarel, Marc J E C, Pijning, Tjaard, Natalia, Dessy, Štefan Janeček, Ihsanawati, Nurachman, Zeily   +20 more
core   +2 more sources

Genome-wide identification and expression analysis of the glycosyl hydrolase family 1 genes in Medicago sativa revealed their potential roles in response to multiple abiotic stresses

BMC Genomics
Glycoside hydrolase family 1 (GH1) β-glucosidases (BGLUs), are encoded by a large number of genes, which participate in the development and stress response of plants, particularly under biotic and abiotic stresses through the activation of phytohormones.
Haiming Kong, Jiaxing Song, Shihai Ma, Jing Yang, Zitong Shao, Qian Li, Zhongxing Li, Zhiguo Xie, Peizhi Yang, Yuman Cao   +9 more
doaj   +1 more source

Predicting Enzyme Turnover Numbers and Enabling Rational Enzyme Evolution

Advanced Science, EarlyView.
MCKcat, a deep learning framework combining multi‐scale convolution and cross‐attention, accurately predicts enzyme turnover number (kcat) by fusing sequence and reaction representations. It couples with rational design in a two‐step strategy for Bacillus aryabhattai laccase engineering, achieving a 75% positive rate with synergistically enhanced ...
Fengya Ge, Xiangyang Ma, Jiyan Li, Mengxiang Wang, Fuping Lu, Yihan Liu, Lin Wang   +6 more
wiley   +1 more source

Reaction Mechanism of Glycoside Hydrolase Family 116 Utilizes Perpendicular Protonation

, 2023
Retaining glycoside hydrolases use acid/base catalysis with an enzymatic acid/base protonating the glycosidic bond oxygen to facilitate leaving-group departure alongside attack by a catalytic nucleophile to form a covalent intermediate.
Gideon J. Davies (367307), Carme Rovira (1275285), Salila Pengthaisong (1519624), James R. Ketudat Cairns (11469818), Beatriz Piniello (9148218)   +4 more
core   +2 more sources