Results 111 to 120 of about 1,574 (145)

Anchoring of Glycosylphosphatidylinositol–Proteins to Liposomes

2003
Publisher Summary This chapter presents the anchoring of glycosylphosphatidylinositol (GPI) proteins to liposomes. In vivo , GPI-anchored proteins concentrate exclusively into the exoleaflet of eukaryotic cell plasma membranes, together with cholesterol and most of the glycolipids.
Olivier, Nosjean, Bernard, Roux
openaire   +2 more sources

Glycosylphosphatidylinositol-phospholipase D: a tool for glycosylphosphatidylinositol structural analysis.

Methods in enzymology, 1995
Cleavage by the GPI-PLD provides definitive evidence of a minimal GPI structure: glucosamine-phosphatidylinositol. Unlike the case for PI-PLC, cleavage by the GPI-PLD is unaffected by acylation of the inositol ring. Thus the GPI-PLD provides an excellent simple enzymatic tool for analyzing the basic core structure of GPI anchors.
M A, Deeg, M A, Davitz
openaire   +1 more source

[45] Glycosylphosphatidylinositol-phospholipase D: A tool for glycosylphosphatidylinositol structural analysis

1995
Publisher Summary This chapter discusses the use of the enzyme glycosylphosphatidylinositol–phospholipase D (GPI-PLD) as a tool for structural analysis of GPI-anchored proteins. The GPI–PLD is a phospholipase D present in all mammalian plasma.
Mark A. Deeg, Michael A. Davitz
openaire   +1 more source

Insertion of a Glycosylphosphatidylinositol-Anchored Enzyme into Liposomes

Journal of Membrane Biology, 2004
Incorporation of alkaline phosphatase (AP), a glycosylphosphatidylinositol (GPI)-anchored protein, into liposomes containing detergent, followed by detergent removal with hydrophobic resin was performed. Incorporation media were collected during different steps of detergent removal and were analyzed by flotation in sucrose gradient.
F, Ronzon, S, Morandat, B, Roux, M, Bortolato   +3 more
openaire   +2 more sources

Glycosylphosphatidylinositol

2013
Howard Riezman, Andreas Conzelmann
openaire   +2 more sources

Glycosylphosphatidylinositol

2018
openaire   +1 more source

Glycosylphosphatidylinositols

2006
openaire   +1 more source

Removal of phospholipid contaminants through precipitation of glycosylphosphatidylinositols

Analytical Biochemistry, 2005
Hosam Shams-Eldin, Nahid Azzouz, Ralph T Schwarz   +2 more
exaly  

Glycosylphosphatidylinositol Anchors and Lipids

2021
Xin-Yu Guo, Morihisa Fujita
openaire   +1 more source

Glycosylphosphatidylinositols

2007
openaire   +1 more source