Results 151 to 160 of about 1,170 (199)

Glycosynthase-Catalysed syntheses at pH below neutrality

Bioorganic & Medicinal Chemistry Letters, 2003
The use of the new glycosynthase Ta-beta-GlyE386G and of the already known Ss-beta-GlyE387G for the synthesis of interesting 4-methylumbelliferyl disaccharides and for the galactosylation of alpha- and beta-xylosides of 4-penten-1-ol is reported. The results show satisfactory yields of reaction in presence of low excesses of acceptors and demonstrated ...
Trincone A, Giordano A, Perugino G, Rossi M, Moracci M   +4 more
openaire   +6 more sources

Glycosynthase-Based Synthesis of Peptidoglycan Oligosaccharides to Address Bacterial Cell-Wall Elongation Processes.

ACS Chemical Biology
Most antibiotics used in human medicine inhibit the biosynthesis of peptidoglycan (PG), which is a vital component of the bacterial cell wall. However, the rapid rise in antimicrobial resistance (AMR) represents a major global health threat.
Antoine Rousseau, D. S. Koffi Teki, Célia Boyat, Emeline Richard, S. Pradeau, Louis Brigandat, Isabel Ayala, J. Simorre, Sylvie Armand, S. Cottaz, C. Bougault, S. Fort   +11 more
semanticscholar   +1 more source

Development of a colourimetric assay for glycosynthases

Journal of Biotechnology, 2017
The synthesis of glycosidic structures by catalysis via glycosynthases has gained much interest due to the potential high product yields and specificity of the enzymes. Nevertheless, the characterisation and implementation of new glycosynthases is greatly hampered by the lack of high-throughput methods for reaction analysis and screening of potential ...
Marc R, Hayes, Kevin A, Bochinsky, Lisa S, Seibt, Lothar, Elling, Jörg, Pietruszka   +4 more
openaire   +2 more sources

Enzymatic transglycosylation of xylose using a glycosynthase

Carbohydrate Research, 2005
The application of the hyperactive glycosynthase derived from Agrobacterium sp. beta-glucosidase (AbgE358G-2F6) to the synthesis of xylo-oligosaccharides by using alpha-D-xylopyranosyl fluoride as donor represents the first successful use of glycosynthase technology for xylosyl transfer.
Young-Wan, Kim, Hongming, Chen, Stephen G, Withers   +2 more
openaire   +2 more sources

Structural basis for transglycosylation in glycoside hydrolase family GH116 glycosynthases

Archives of Biochemistry and Biophysics, 2021
Glycosynthases are glycoside hydrolase mutants that can synthesize oligosaccharides or glycosides from an inverted donor without hydrolysis of the products. Although glycosynthases have been characterized from a variety of glycoside hydrolase (GH) families, family GH116 glycosynthases have yet to be reported.
Salila, Pengthaisong, Yanling, Hua, James R, Ketudat Cairns   +2 more
openaire   +2 more sources

Glycosynthase‐Mediated Assembly of Xylanase Substrates and Inhibitors

ChemBioChem, 2011
AbstractAn exo‐β‐xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were easily elaborated into di‐, tri‐ and tetrasaccharides by using this enzyme. Some products proved to be surprisingly potent inhibitors of xylanases from glycoside hydrolase families 10 and ...
Ethan D, Goddard-Borger, Brigitte, Fiege, Emily M, Kwan, Stephen G, Withers   +3 more
openaire   +2 more sources

Structural explanation for the acquisition of glycosynthase activity

The Journal of Biochemistry, 2010
Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors. We have determined five crystal structures of the glycosynthase mutants reducing-end xylose-releasing exo-oligoxylanase, an inverting GH, that exhibit various levels of glycosynthetic activities. At the
Masafumi, Hidaka, Shinya, Fushinobu, Yuji, Honda, Takayoshi, Wakagi, Hirofumi, Shoun, Motomitsu, Kitaoka   +5 more
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1.6.1 Glycosidases and Glycosynthases

2015
AbstractEnzymatic synthesis of glycans, as an alternative to classical chemical synthesis, is of great interest due to the exquisite stereospecificity and improved processivity and regioselectivity of the biological catalysts, and for the possibility of using reagents less toxic to the environment.
B. Cobucci Ponzano, M. Moracci
openaire   +1 more source

Directed Evolution of a Glycosynthase via Chemical Complementation

Journal of the American Chemical Society, 2004
Recently, we reported a general assay for enzyme catalysis based on the yeast three-hybrid assay, Chemical Complementation, which is intended to expand the range of chemical reactions to which directed evolution can be applied. Here, Chemical Complementation was applied to a glycosynthase derived from a retaining glycosidase, an important class of ...
Hening, Lin, Haiyan, Tao, Virginia W, Cornish   +2 more
openaire   +2 more sources

Glycosynthases: new enzymes for oligosaccharide synthesis

Journal of Molecular Catalysis B: Enzymatic, 2001
The mutation of putative acidrbase and nucleophile of the active sites of retaining glycosyl hydrolases, together with kinetic analysis of the mutants, and stereochemical identification of products lead to useful information for the understanding of the reaction mechanism of these enzymes.
Moracci M, Trincone A, Rossi, M
openaire   +5 more sources