Results 111 to 120 of about 505,963 (341)

Enhanced small green fluorescent proteins as a multisensing platform for biosensor development. [PDF]

Front Bioeng Biotechnol, 2022
Liang GT, Lai C, Yue Z, Zhang H, Li D, Chen Z, Lu X, Tao L, Subach FV, Piatkevich KD.   +9 more
europepmc   +1 more source

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

Rational Design for High Bioorthogonal Fluorogenicity of Tetrazine-Encoded Green Fluorescent Proteins. [PDF]

Nat Sci (Weinh), 2022
Tang L, Bednar RM, Rozanov ND, Hemshorn ML, Mehl RA, Fang C.   +5 more
europepmc   +1 more source

Two-step excitation of fluorescent proteins with real intermediary states [PDF]

arXiv
We demonstrate sequential two-photon fluorescence microscopy using forbidden state transitions. Nonlinear red excitation leads to green fluorescence in live cells expressing eYFP, maintaining optical sectioning and allowing deep tissue imaging with simple optical systems.
arxiv  

Structure of the chromophore of Aequorea green fluorescent protein [PDF]

, 1979
Osamu Shimomura
openalex   +1 more source

Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells

Molecular Oncology, EarlyView.
Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, Gökhan Karakülah, Serif Senturk   +11 more
wiley   +1 more source

Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants

Frontiers in Plant Science, 2018
Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein ...
Bianca Baudisch, Ingrid Pfort, Eberhard Sorge, Udo Conrad   +3 more
doaj   +1 more source

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, EarlyView.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

Improved split fluorescent proteins for endogenous protein labeling

Nature Communications, 2017
Split fluorescent proteins (FPs) have been widely used to visualise proteins in cells. Here the authors develop a screen for engineering new split FPs, and report a yellow-green split-mNeonGreen2 with reduced background, a red split-sfCherry2 for ...
Siyu Feng, Sayaka Sekine, Veronica Pessino, Han Li, Manuel D. Leonetti, Bo Huang   +5 more
doaj   +1 more source

Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis [PDF]

, 1995
Chris Webb, Amy L. Decatur, Aurelio A. Teleman, Richard Losick   +3 more
openalex   +1 more source