Results 81 to 90 of about 723,125 (296)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

A distributed alerting service for open digital library software [PDF]

, 2005
Alerting for Digital Libraries (DL) is an important and useful feature for the library users. To date, two independent services and a few publisher-hosted proprietary services have been developed.
Buchanan, George, Hinze, Annika
core   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre. [PDF]

, 2019
Sinking particles transport carbon and nutrients from the surface ocean into the deep sea and are considered hot spots for bacterial diversity and activity.
Collins, James R, Farnelid, Hanna, Ossolinski, Justin E, Ploug, Helle, Turk-Kubo, Kendra, Van Mooy, Benjamin AS, Zehr, Jonathan P   +6 more
core   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

The Green Library

, 2015
Fachleute zeigen in übergreifenden Beiträgen und anhand von konkreten Fallbeispielen sowohl aus Deutschland wie aus anderen Teilen Europas, Asiens, Australiens und den USA, wie der ökologische Fußabdruck im Alltag der Bibliothek verringert werden kann und was Nachhaltigkeit für die soziale Verantwortung von Bibliotheken in ihrer Rolle als ...
Sommer, Dorothea, Hauke, Petra, Latimer, Karen, Werner, Klaus Ulrich, Aldrich, Rebekkah S., Benton, Susan, Schaper, Louise, Scherer, Jeffrey A., Genovese, Peter   +8 more
openaire   +1 more source

Comparative Genomic Characterization of the Multimammate Mouse Mastomys coucha. [PDF]

, 2019
Mastomys are the most widespread African rodent and carriers of various diseases such as the plague or Lassa virus. In addition, mastomys have rapidly gained a large number of mammary glands.
Aaron Hardin, Andersen, Bao, Besemer, Bickhart, Blanchette, Bolger, Bonwitt, Booker, Bourque, Camacho, Capra, Chapman, Chen, Chikhi, Chu, Colangelo, Dewey, Dierckxsens, Eblaghie, Grabherr, Hayssen, Heinz, Helfrich, Holloway, Holt, Jiang, Kannan, Kiełbasa, Kim, Kimberly A Nevonen, Kolmogorov, Korf, Krueger, Lecompte, Li, Li, Lok, Lowe, Lucia Carbone, MacManes, McLean, Modlin, Nadav Ahituv, Nagy, Nilsson, Närhi, Pennacchio, Pertea, Pimentel, Pollard, Sands, Schep, Scott, Siepel, Siepel, Simão, Smit, Snell, Song, Stanke, UniProt Consortium, Van der Auwera, Veltmaat, Veltmaat, Walter L Eckalbar   +65 more
core   +1 more source

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source