Results 261 to 270 of about 60,004 (331)

Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides

Plant Biotechnology Journal, EarlyView.
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew, Aron Ferenczi, Marie Dannay, Cristina Ponce‐Lilly, Adam Kovac, Dávid Tóth, Szilvia Z. Tóth, Attila Molnar   +7 more
wiley   +1 more source

Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells. [PDF]

STAR Protoc
Adami A, Garza R, Dorazehi F, Douse CH, Jakobsson J.   +4 more
europepmc   +1 more source

Optimisation of Agrobacterium‐Mediated Seedling Leaf Transformation Across Multiple Sorghum Genotypes

Plant Biotechnology Journal, EarlyView.
Mercy K. Azanu, Keunsub Lee, Kan Wang
wiley   +1 more source

Bibliometric‐Based Analysis of Global Trends and Collaborative Networks in Plant Genetic Engineering (1994–2024)

Plant Biotechnology Journal, EarlyView.
ABSTRACT Agricultural sustainability faces serious challenges from population growth, climate change and ecological degradation. Genetic modification (GM) technology can be regarded as a precise extension of the Green Revolution, aiming to balance yield enhancement with ecological integrity through biotechnology. To systematically examine global trend,
Tongxiao Xu, Teng Wang, Cong Zhang, Yuan Cao, Xiaoyun He   +4 more
wiley   +1 more source

Multilayered regulation by RNA thermometers enables precise control of Cas9 expression in E. coli. [PDF]

Nucleic Acids Res
Kammerdiener EK, Garcia SK, Bales MK, Klingeman DM, Guss AM, Giannone RJ, Hettich RL, Eckert CA, Alexander WG.   +8 more
europepmc   +1 more source

Rewiring Steroidal Metabolic Pathways for Diosgenin Production in Solanum nigrum

Plant Biotechnology Journal, EarlyView.
ABSTRACT Diosgenin is a key starting material for the synthesis of steroidal drugs, such as corticosteroids and sex hormones. While the primary commercial source of diosgenin is the tubers of Dioscorea spp., identifying alternative plant hosts capable of diosgenin biosynthesis could enhance its production.
Jongbu Lim, Keunhwa Kim, Jung Heo, Seung Mo Seo, Sungjun Choung, Hyeonjin Kim, Yuri Choi, Kyungsun Park, Hyejung Yun, Dongha Kim, Eun Song Lee, Junwoo Lee, Smita Mirsyad Warsadiharja, Saet Buyl Lee, Sunkyu Han, Soon Ju Park, Sang‐Gyu Kim   +16 more
wiley   +1 more source

Creating Loss-of-Function Mutants of <i>Neurospora crassa</i> Using a Novel CRISPR/Cas9 System. [PDF]

Bio Protoc
Grüttner S.
europepmc   +1 more source

Democratising high performance computing for bioinformatics through serverless cloud computing: A case study on CRISPR-Cas9 guide RNA design with Crackling Cloud. [PDF]

PLoS Comput Biol
Bradford J, Joy D, Winsen M, Meurant N, Wilkins M, Wilson LOW, Bauer DC, Perrin D.   +7 more
europepmc   +1 more source

CRISPR/Cas9‐Mediated Specific Knockout of GhCesA4/7/8 Improves Cotton Fibre Quality

Plant Biotechnology Journal, EarlyView.
Lu Qiao, Haozhe Tan, Zhengxiu Ye, Shuangxia Jin, Xianlong Zhang, Lili Tu   +5 more
wiley   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source