Results 161 to 170 of about 573,666 (268)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Ground state energy of a dilute Bose gas with three-body hard-core interactions. [PDF]

Lett Math Phys
Junge L, Visconti FLA.
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Delicate inhibition of NFκB/RELA circuitry facilitates efficient transition towards ground-state pluripotency in human. [PDF]

Nat Commun
Wang L, Xiao L, Zou G, Mao H, Zhang S, Yang Q, Yin C, Wan J, Kumar M, Wu H, Wang H, Zhang X, Zeng L, Zhou C, Yu S, Li Y, Zheng L, Liu Y, Liao B, Zhang Z, Liu J.   +20 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Solution-synthesized stable triaza[4]triangulene triradical with a quartet ground state. [PDF]

Nat Commun
Bai X, Zhang D, Zhang Y, Wang Y, Cheng Y, Xie Y, Zhu Z, Jiang SD, Zhao D.   +8 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Multiconfigurational Ground State of a Diradicaloid Characterized at the Atomic Scale. [PDF]

J Am Chem Soc
Turco E, Tejerina L, Catarina G, Ortega-Guerrero A, Krane N, Gross L, Juríček M, Mishra S.   +7 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Single-Determinant Ground State in Ce⁴⁺ Imidophosphorane Complexes. [PDF]

Inorg Chem
Tateyama H, Liao C, Wilkinson GR, Ramanathan A, Amidani L, Bazarkina E, Ressnik F, Engle KS, Bacsa J, Sokaras D, Kvashnina KO, Li X, La Pierre HS.   +12 more
europepmc   +1 more source