Results 211 to 220 of about 388,991 (266)

CREAT: A CRISPR‐Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction

Advanced Science, EarlyView.
Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan, Zhe Gao, Yingxuan Qi, Yang Zhang, Xuhui Tian, Pengpeng Zhao, Xu Feng, Qunxin She   +7 more
wiley   +1 more source

A genetic manipulation tool based on the GP35 recombinase for targeted gene editing in mycoplasmas of ruminants. [PDF]

J Biol Chem
Lan S, Li Z, Jing T, Cui W, Zhang Y, Hao H, Chen R, Baz AA, Tian X, Askar H, Li B, Yan X, Gao P, Chen S, Chu Y.   +14 more
europepmc   +1 more source

Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing

Advanced Science, EarlyView.
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Xi Luo, Yuyao Qu, Zhengyan Ye, Zheng Li, Yuanyan Zhang, Longjiong Luo, Shaokang Li, Wei Zhao, Ming Wang, Ralph Bock, Jianmin Wan, Junjie Tan   +11 more
wiley   +1 more source

SorghumBase: a knowledgebase for sorghum genomics, phenomics, and stakeholder engagement. [PDF]

Genetics
Gladman N, Olson A, Kumari S, Wei S, Chougule K, Lu Z, Tello-Ruiz MK, Van Buren P, Kumar V, Zhang L, Olson A, Kim C, Braynen J, Hayes C, Xin Z, Klein R, Rooney W, Provart N, Pasha A, O'Meara A, Shakoor N, Michael TP, Harrison M, Ware D.   +23 more
europepmc   +1 more source

The Spatiotemporal Genetic Architecture of Seed Vigor in Upland Cotton

Advanced Science, EarlyView.
Leveraging the semi‐automated SeedRanger platform, we profiled the germination kinetics of 356 cotton accessions at a 30‐min interval. This high‐throughput phenomic approach delineated a temporal genetic network comprising 541 stage‐specific loci. Crucially, functional validation identified FLA2 as a pivotal, auxin‐modulated regulator that orchestrates
Luyao Wang, Jie Dai, Ting Zhao, Shengjun Zhao, Hongyu Wu, Jiaying Pan, Yupeng Hao, Ke Nie, Li Yu, Yumeng Zhu, Xiangyi Zhao, Kai Huang, Mengke Zhang, Yongyan Zhao, Yaping Jiang, Siyuan Wang, Yiling Pan, Yubing Chen, Yilai Lin, Wanghong Shi, Yayuan Deng, Jin Han, Jie Li, Xuan Long, Junbin Wang, Zhiyuan Zhang, Zegang Han, Zhanfeng Si, Lei Ju, Jun Li, Tianzhen Zhang, Ji Zhou, Xueying Guan   +32 more
wiley   +1 more source

Correction: A transmission relationship investigation of HIV infection through male-to-male sex among a case of left-behind children with heterosexual orientation in Zhejiang Province of China. [PDF]

Front Public Health
Hou Z, Zhang J, Feng H, Chen Z, Fan Q, Pan X, Zhu G, Ge R.   +7 more
europepmc   +1 more source

The E3 Ligase RNF115 Aggravates Pathological Cardiac Hypertrophy via Ubiquitin‐Mediated Degradation of SPTBN1

Advanced Science, EarlyView.
In response to hypertrophic stimuli, increased c‑JUN phosphorylation upregulates RNF115, leading to SPTBN1 ubiquitination and degradation. which promotes F‑actin depolymerization and YAP activation, driving cardiac hypertrophy. The RNF115 inhibitor DTD effectively suppresses SPTBN1 ubiquitination and cardiac hypertrophy.
Yan Zu, Shiqing Chen, Ji Chen, Liuliu Ruan, Bowen Li, Weijian Chu, Shichen Huang, Dian Yu, Jing Tian, Yuhan Zhao, Yi Han, Xin Tang, Yong Ji   +12 more
wiley   +1 more source

Age-dependent patterns of cardiac complexity unveiled by topological data analysis of pediatric heart rate variability. [PDF]

PLoS One
Domínguez-Monterroza A, Mateos Caballero A, Jiménez-Martín A.   +2 more
europepmc   +1 more source

Lessons learned from manual curation of thousands of gene models in the nematode Pristionchus pacificus. [PDF]

PLoS One
Rödelsperger C, Agyal N, Quiobe SP, Wu H, Röseler W, Ibarra-Morales D, Sommer RJ.   +6 more
europepmc   +1 more source

Topological data analysis and topological deep learning beyond persistent homology: a review. [PDF]

Artif Intell Rev
Su Z, Liu X, Hamdan LB, Maroulas V, Wu J, Carlsson G, Wei GW, Wei GW.   +7 more
europepmc   +1 more source