Cloning of the alpha-subunit of GS protein from spontaneously hypertensive rats. [PDF]
Hypertension, 1994 Enhanced sodium reabsorption by the kidney has a significant role in the development of genetic hypertension. In the spontaneously hypertensive rat (SHR) model of genetic hypertension, the enhanced sodium reabsorption likely arises from abnormal hormonal regulation of tubular transport.
Richard W. Gurich +2 more
openalex +4 more sources
DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding (
BMC Genetics, 2011 Background Genes which are epigenetically regulated via genomic imprinting can be potential targets for artificial selection during animal breeding. Indeed, imprinted loci have been shown to underlie some important quantitative traits in domestic mammals,
Mullen Michael P +8 more
doaj +2 more sources
Further evidence supporting a role for gs signal transduction in severe malaria pathogenesis. [PDF]
PLoS ONE, 2010 With the functional demonstration of a role in erythrocyte invasion by Plasmodium falciparum parasites, implications in the aetiology of common conditions that prevail in individuals of African origin, and a wealth of pharmacological knowledge, the ...
Sarah Auburn +14 more
doaj +6 more sources
ADP-ribosylation of Gs promotes the dissociation of its alpha and beta subunits.
Journal of Biological Chemistry, 1984 We have utilized purified reactants and cofactors to examine the form of the stimulatory guanine nucleotide-binding regulatory component (Gs) of adenylate cyclase that serves as a substrate for ADP-ribosylation by cholera toxin; we have also investigated some of the consequences of that covalent modification.
Richard Kahn, Alfred G. Gilman
openalex +4 more sources
Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity. [PDF]
The Journal of cell biology, 1992 Binding of GTP induces alpha subunits of heterotrimeric G proteins to take on an active conformation, capable of regulating effector molecules. We expressed epitope-tagged versions of the alpha subunit (alpha s) of Gs in genetically alpha s-deficient S49 cyc- cells. Addition of a hemagglutinin (HA) epitope did not alter the ability of wild type alpha s
Mark J. Levis, Henry R. Bourne
openalex +4 more sources
Mutation of valine residue unique to alpha subunit of Gs abolishes activation.
Journal of Biological Chemistry, 1994 We recently characterized a decapeptide sequence (residues 367-376) that is important for the membrane association of the activated alpha subunit of Gs. We report here that when this sequence is replaced by the cognate sequence of Gi1 alpha subunit, the chimeric protein (Gsis alpha) still interacts with the membrane but cannot be activated, regardless ...
Eric Devic +4 more
openalex +4 more sources
The G226A mutant of Gs alpha highlights the requirement for dissociation of G protein subunits.
Journal of Biological Chemistry, 1992 Adenylylcyclase cannot be activated by hormones or guanine nucleotide analogs in membranes from cells that express the G226A mutant form Gs alpha instead of the wild-type protein. The mutant Gs alpha protein appears incapable of undergoing the conformational change necessary for guanine nucleotide-induced dissociation of the G protein alpha subunit ...
E Lee, Ronald Taussig, Alfred G. Gilman
openalex +4 more sources
Competing activities of heterotrimeric G proteins in Drosophila wing maturation. [PDF]
PLoS ONE, 2010 Drosophila genome encodes six alpha-subunits of heterotrimeric G proteins. The Galphas alpha-subunit is involved in the post-eclosion wing maturation, which consists of the epithelial-mesenchymal transition and cell death, accompanied by unfolding of the
Natalya Katanayeva +4 more
doaj +6 more sources
Reticulocyte lysates synthesize an active alpha subunit of the stimulatory G protein Gs.
Journal of Biological Chemistry, 1988 We placed the cDNAs encoding one of the short types of alpha s (alpha s-1) with Asp-Ser in positions 70 and 71 and one of the long types of alpha s (alpha s-2) in which Asp-Ser are substituted with a string of 16 amino acids, into the pGEM-3 transcription vector downstream from its T7 RNA polymerase promoter, obtained transcripts and translated the ...
Juan Olate +3 more
openalex +3 more sources
Amino acids 367-376 of the Gs alpha subunit induce membrane association when fused to soluble amino-terminal deleted Gi1 alpha subunit. [PDF]
Proceedings of the National Academy of Sciences, 1991 Signal transduction GTP-binding proteins are tightly associated with plasma membrane. In the resting state, the anchorage of the alpha subunit could be indirect by means of the other beta gamma subunits or polydisperse multimers. In the activated state, although the alpha subunit is dissociated from other subunits, it is not released from the membrane ...
Laurent Journot +5 more
openalex +4 more sources