Results 211 to 220 of about 63,776 (234)

VIP Induces the Translocation and Degradation of the   Subunit of Gs Protein in Rat Pituitary GH4C1 Cells

Journal of Biochemistry, 1998
It has been shown that G proteins are potential regulatory molecules in the transmembrane signaling cascade. The aim of this study was to examine the possibility of equivalent G-protein redistribution and/or down-regulation in a target cell upon agonist stimulation.
Seiichi Kawashima, Toshikazu Saito, Yoshiko Akita, Yukiko Yajima   +3 more
openaire   +3 more sources

The effect of iloprost on the ADP-ribosylation of Gs alpha (the alpha-subunit of Gs).

The Biochemical journal, 1989
Treatment of platelets with a prostacyclin analogue, iloprost, decreased the cholera-toxin-induced ADP-ribosylation of membrane-bound Gs alpha (alpha-subunit of G-protein that stimulates adenylate cyclase; 42 kDa protein) and a cytosolic substrate (44 kDa protein) [Molina y Vedia, Reep & Lapetina (1988) Proc. Natl. Acad. Sci. U.S.A.
L, Molina y Vedia, R D, Nolan, E G, Lapetina   +2 more
openaire   +1 more source

Overexpression of Gs alpha subunit in thyroid tumors bearing a mutated Gs alpha gene.

Journal of cancer research and clinical oncology, 1995
Point mutations occurring at codon 201 of the gene coding for the alpha subunit of the stimulatory G protein impair intrinsic GTPase activity and lead to a constitutive activation of adenylate cyclase. We have examined thyroid follicular and papillary carcinomas and follicular adenomas and found samples that bear this mutation at codon 201 of the Gs ...
V N, Gorelov, K, Dumon, N S, Barteneva, D, Palm, H D, Röher, P E, Goretzki   +5 more
openaire   +1 more source

Altered Gsα N-terminus affects Gs activity and interaction with the Gβγ subunit complex in cell membranes but not in solution

Cellular Signalling, 1996
The stimulatory G protein (Gs) mediates activation of adenylylcyclase by a ligand-receptor complex. Gs is heterotrimeric (alpha beta gamma) and activation can be accomplished by dissociation of the alpha-subunit (Gs alpha) from the beta gamma-subunit complex (G beta gamma).
S Okuya, R V Rebois, D R Warner
openaire   +3 more sources

A unique mRNA species for the α subunit of GS is present in rat haploid germ cells

Biochemical and Biophysical Research Communications, 1990
The 1.9 kb mRNA encoding the alpha subunit of Gs observed in the somatic cells of the rat testis was not seen in the germ cells (pachytene spermatocytes and round spermatids) as revealed by Northern blot analysis. Interestingly, the cDNA probe for Gs alpha detected a mRNA of shorter chain length (0.9 kb) exclusively in the haploid germ cells (round and
Trine B. Haugen, Jan O. Gordeladze, Eyvind J. Paulssen, Vidar Hansson   +3 more
openaire   +3 more sources

Postmortem cerebral cortex Gs α-subunit levels are elevated in bipolar affective disorder

Brain Research, 1991
We examined the relative abundance of G-protein subunits in postmortem brain obtained from 7 patients with bipolar affective disorder (BAD) compared with 7 age- and sex-matched controls. G-protein subunit immunoreactivities were determined in membranes prepared from postmortem prefrontal cortex using SDS-PAGE and immunoblotting with specific polyclonal
Peter P. Li, K.P. Siu, Stephen J. Kish, L. T. Young, Jerry J. Warsh   +4 more
openaire   +3 more sources

Extralarge XL(alpha)s (XXL(alpha)s), a variant of stimulatory G protein alpha-subunit (Gs(alpha)), is a distinct, membrane-anchored GNAS product that can mimic Gs(alpha).

Endocrinology, 2009
GNAS gives rise to multiple imprinted gene products, including the alpha-subunit of the stimulatory G protein (Gs(alpha)) and its variant XL(alpha)s. Based on genomic sequence, the translation of XL(alpha)s begins from the middle of a long open reading frame, suggesting the existence of an N-terminally extended variant termed extralarge XLalphas (XXL ...
Cumhur, Aydin, Nurgul, Aytan, Mathew J, Mahon, Hesham A W, Tawfeek, Neil W, Kowall, Alpaslan, Dedeoglu, Murat, Bastepe   +6 more
openaire   +1 more source

Exclusion of close linkage of bipolar disorder to the Gs-α subunit gene in nine Australian pedigrees

Journal of Affective Disorders, 1994
Growing evidence suggests that guanine nucleotide binding proteins (G proteins) may be involved in both the pathogenesis and treatment of bipolar affective disorder. Both overactive G proteins and increased levels of the alpha subunit of the stimulatory form (Gs-alpha) have been demonstrated in peripheral leucocytes of manic patients while an increase ...
Christina Vivero, Lisa Selbie, F. Le, John Shine, Brent Waters, Peter R. Schofield, Philip B. Mitchell, Jennifer A. Donald   +7 more
openaire   +3 more sources

Reciprocal regulation of Gs alpha by palmitate and the beta gamma subunit.

Proceedings of the National Academy of Sciences of the United States of America, 1997
Hormonal activation of Gs, the stimulatory regulator of adenylyl cyclase, promotes dissociation of alpha s from G beta gamma, accelerates removal of covalently attached palmitate from the G alpha subunit, and triggers release of a fraction of alpha s from the plasma membrane into the cytosol. To elucidate relations among these three events, we assessed
T, Iiri, P S, Backlund, T L, Jones, P B, Wedegaertner, H R, Bourne   +4 more
openaire   +1 more source

[19] Assays for studying functional properties of in vitro translated Gsα subunit

1994
Publisher Summary This chapter discusses different procedure designed to study the functional properties of mutated in vitro translated Gsα subunits. The relationship between the structure and function of a protein has been widely explored by in vitro mutagenesis of the cDNA encoding the protein and expression of the mutated protein in an eukaryotic ...
openaire   +3 more sources