Results 11 to 20 of about 221,192 (198)
The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability [PDF]
eLife, 2019 ABSTRACTMicrotubules are bistable cytoskeletal polymers whose function depends on their property to switch between states of growth and shrinkage1. Growing microtubules are thought to be stabilized by a GTP cap at their ends2-5. The nature of this cap, however, is still poorly understood.
Johanna Roostalu +5 more
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GTP: Gatekeeper for Autophagy [PDF]
Molecular Cell, 2010 Chaperone-mediated autophagy (CMA) is involved in cellular quality control, and in the response to stress, deterioration in CMA contributes to the aging phenotype and to various disorders. The paper by Bandyopadhyay et al. in this issue of Molecular Cell demonstrates the role of GTP binding by elongation factor 1 alpha (EF1alpha) in regulating the ...
Isabelle Beau, Patrice Codogno
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Journal of Biological Chemistry, 2002 Because of the apparently greater conformational flexibility of yeast versus muscle actin and the ability of other members in the actin protein superfamily to efficiently use both ATP and GTP, we assessed the ability of yeast actin to function with GTP.
Xiaoyi Yao +2 more
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Kinetic analysis of GTP hydrolysis catalysed by the Arf1-GTP-ASAP1 complex [PDF]
Biochemical Journal, 2007 Arf (ADP-ribosylation factor) GAPs (GTPase-activating proteins) are enzymes that catalyse the hydrolysis of GTP bound to the small GTP-binding protein Arf. They have also been proposed to function as Arf effectors and oncogenes. We have set out to characterize the kinetics of the GAP-induced GTP hydrolysis using a truncated form of ASAP1 [Arf GAP with ...
Ruibai Luo +6 more
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RalA and RalB Function as the Critical GTP Sensors for GTP-Dependent Exocytosis [PDF]
The Journal of Neuroscience, 2007 Although it has been established that the activation of GTPases by non-hydrolyzable GTP stimulates neurotransmitter release from many different secretory cell types, the underlying mechanisms remain unclear. In the present study we aimed to elucidate the functional role(s) for endogenous Ras-like protein A (RalA) and RalB GTPases in GTP-dependent ...
Lakshmanan Arunachalam +10 more
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Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading
Nature, 2020 Ribosomes accurately decode mRNA by proofreading each aminoacyl-tRNA that is delivered by the elongation factor EF-Tu 1 . To understand the molecular mechanism of this proofreading step it is necessary to visualize GTP-catalysed elongation, which has ...
A. Loveland, G. Demo, A. Korostelev
semanticscholar +1 more source
GTP cyclohydrolase I utilizes metal‐free GTP as its substrate [PDF]
European Journal of Biochemistry, 2003 GTP cyclohydrolase I (GCH) is the rate‐limiting enzyme for the synthesis of tetrahydrobiopterin and its activity is important in the regulation of monoamine neurotransmitters such as dopamine, norepinephrine and serotonin. We have studied the action of divalent cations on the enzyme activity of purified recombinant human GCH expressed in Escherichia ...
T. Suzuki, H. Kurita, H. Ichinose
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GTP Cyclohydrolase 1/Tetrahydrobiopterin Counteract Ferroptosis through Lipid Remodeling
ACS Central Science, 2019 Ferroptosis is an iron-dependent form of regulated cell death linking iron, lipid, and glutathione levels to degenerative processes and tumor suppression.
Vanessa A N Kraft +16 more
semanticscholar +1 more source
Analytical Chemistry, 2014 Thiopurine drugs are widely used as antileukemic drugs and immunosuppressive agents, and 6-thioguanosine triphosphate ((S)GTP) is a major metabolite for these drugs. Recent studies have suggested that thiopurine drugs may exert their cytotoxic effects partly through binding of (S)GTP to a GTP-binding protein, Rac1.
Debin Ji +3 more
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Mitochondria-lysosome contacts regulate mitochondrial fission via Rab7 GTP hydrolysis
Nature, 2018 Both mitochondria and lysosomes are essential for maintaining cellular homeostasis, and dysfunction of both organelles has been observed in multiple diseases.
Y. Wong, Daniel Ysselstein, D. Krainc
semanticscholar +1 more source