Guanosine 5'-o- 3-thiotriphosphate

Results 121 to 130 of about 2,776 (139)

Reconstitution of catecholamine-stimulated binding of guanosine 5'-O-(3-thiotriphosphate) to the stimulatory GTP-binding protein of adenylate cyclase [PDF]

Biochemistry, 1984
The stimulatory GTP-binding protein (Gs) of adenylate cyclase, purified from rabbit liver, and beta-adrenergic receptors, partially purified 1000-4000-fold from turkey erythrocyte plasma membranes, were coreconstituted into unilamellar phospholipid vesicles.
Tomiko Asano +3 more
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Magnesium requirements for guanosine 5'-O-(3-thiotriphosphate) induced assembly of microtubule protein and tubulin

Biochemistry, 1986
Two conflicting interpretations on the role of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) in microtubule protein and tubulin assembly have been previously reported. One study finds that GTP gamma S promotes assembly while another study reports that GTP gamma S is a potent inhibitor of microtubule assembly.
Sukla Roychowdhury, Felicia Gaskin
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Characterization, Guanosine 5′-O-(3-Thiotriphosphate) Modulation, Daily Variation, and Localization of Melatonin-Binding Sites in the Catfish (Silurus asotus) Brain

General and Comparative Endocrinology, 1997
Characteristics, guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) modulation, daily variation, and localization of melatonin-binding sites in the brain of a nocturnal teleost, the catfish Silurus asotus, were studied by radioreceptor assay using 2-[125I]iodomelatonin as the radioligand.
Masayuki Iigo +7 more
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Activation of 5-lipoxygenase by guanosine 5′-O-(3-thiotriphosphate) and other nucleoside phosphorothioates: Redox properties of thionucleotide analogs

Archives of Biochemistry and Biophysics, 1989
The stable nucleotide analog guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) was found to be a very potent activator of 5-lipoxygenase in cell-free preparations from rat polymorphonuclear (PMN) leukocytes, causing a 10-fold stimulation of arachidonic acid oxidation at concentrations as low as 0.5-1 microM.
Danielle Denis, Lancelot Y. Choo, Denis Riendeau +2 more
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Caffeine inhibits cytoplasmic Ca2+ oscillations induced by carbachol and guanosine 5′-O-(3-thiotriphosphate) in hyperpolarized pancreatic ß-cells

Naunyn-Schmiedeberg's Archives of Pharmacology, 1994
The effects of caffeine on cytoplasmic Ca2+ oscillations induced by carbachol and guanosine 5'-O-(3-thiotriphosphate) (GTP-gamma-S) were studied in individual mouse pancreatic beta-cells clamped at a hyperpolarized potential. Addition of 10 mM caffeine did not affect the cytoplasmic Ca2+ concentration ([Ca2+]i) in beta-cells exposed to 20 mM glucose ...
Per-Eric Lund, Erik Gylfe
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Kinetic analysis of guanosine 5'-O-(3-thiotriphosphate) effects on phosphatidylinositol turnover in NRK cell homogenates

Biochemistry, 1987
Addition of the guanine nucleotide analogue guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to [3H]inositol-labeled NRK cell homogenates resulted in rapid breakdown of cellular polyphosphoinositides. GTP gamma S stimulated phospholipase C, resulting in a more than 4-fold increase in the hydrolysis rates of phosphatidylinositol 4-phosphate (PIP) and ...
Suresh B. Chahwala +2 more
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Plasma membrane associated phospholipase C from human platelets: synergistic stimulation of phosphatidylinositol 4,5-bisphosphate hydrolysis by thrombin and guanosine 5'-O-(3-thiotriphosphate)

Biochemistry, 1989
The effects of thrombin and GTP gamma S on the hydrolysis of phosphoinositides by membrane-associated phospholipase C (PLC) from human platelets were examined with endogenous [3H]inositol-labeled membranes or with lipid vesicles containing either [3H]phosphatidylinositol or [3H]phosphatidylinositol 4,5-bisphosphate.
Joseph J. Baldassare, Patricia Henderson, Gary J. Fisher +2 more
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Guanosine 5′-O-(3-Thiotriphosphate)-induced O−2Generation in Permeabilized Neutrophils Requires Protein Kinase C and Phospholipase C but Not Tyrosine Kinase or Phospholipase D

Archives of Biochemistry and Biophysics, 1999
Guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) induces respiratory burst (O-2 generation) in permeabilized human neutrophils. The signal pathway from GTPgammaS to the enzyme responsible for O-2 generation (NADPH oxidase) is not well defined. To elucidate the signaling pathway activated by GTPgammaS, we used selective inhibitors to test for the ...
Minoru Tamura, Kenji Yoshida, Kei KATAOKA +2 more
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Effect of Guanosine 5′‐O‐(3‐Thiotriphosphate) and Calcium on γ‐Aminobutyric Acid_B Binding as a Function of Postnatal Development

Journal of Neurochemistry, 1989
Abstract: We have examined the development of γ‐aminobutyric acidB (GABAB) receptors in rat cerebrum using a binding assay that has achieved specific binding levels of ∼50% with the GABAB ligand (–)‐[3H]baclofen. As early as postnatal day 1, GABAB receptors are present and are linked to both calcium‐ and guanosine triphosphate‐binding protein (G ...
Muna I. Al‐Dahan, Robert H. Thalmann
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Combination of Arachidonic Acid and Guanosine 5′-O-(3-Thiotriphosphate) Induces Translocation of rac p21s to Membrane and Activation of NADPH Oxidase in a Cell-Free System

Biochemical and Biophysical Research Communications, 1993
The superoxide-generating NADPH oxidase system in phagocytes consists of membrane-associated cytochrome b558 and three cytosolic components named p67-phox, p47-phox, and rac p21s. In a cell-free system consisting of membrane and cytosol, the oxidase can be activated with guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and an unsaturated fatty acid ...
T. Sawai +8 more
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biology
enzyme
animals

gtp'
guanosine triphosphate
g protein

kinetics
receptor
thionucleotides