Results 111 to 120 of about 37,457,458 (124)

Reconstitution of catecholamine-stimulated binding of guanosine 5'-O-(3-thiotriphosphate) to the stimulatory GTP-binding protein of adenylate cyclase

Biochemistry, 1984
The stimulatory GTP-binding protein (Gs) of adenylate cyclase, purified from rabbit liver, and beta-adrenergic receptors, partially purified 1000-4000-fold from turkey erythrocyte plasma membranes, were coreconstituted into unilamellar phospholipid vesicles.
T, Asano, S E, Pedersen, C W, Scott, E M, Ross   +3 more
openaire   +2 more sources

Activation of 5-lipoxygenase by guanosine 5′-O-(3-thiotriphosphate) and other nucleoside phosphorothioates: Redox properties of thionucleotide analogs

Archives of Biochemistry and Biophysics, 1989
The stable nucleotide analog guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) was found to be a very potent activator of 5-lipoxygenase in cell-free preparations from rat polymorphonuclear (PMN) leukocytes, causing a 10-fold stimulation of arachidonic acid oxidation at concentrations as low as 0.5-1 microM.
D, Denis, L Y, Choo, D, Riendeau
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Caffeine inhibits cytoplasmic Ca2+ oscillations induced by carbachol and guanosine 5′-O-(3-thiotriphosphate) in hyperpolarized pancreatic ß-cells

Naunyn-Schmiedeberg's Archives of Pharmacology, 1994
The effects of caffeine on cytoplasmic Ca2+ oscillations induced by carbachol and guanosine 5'-O-(3-thiotriphosphate) (GTP-gamma-S) were studied in individual mouse pancreatic beta-cells clamped at a hyperpolarized potential. Addition of 10 mM caffeine did not affect the cytoplasmic Ca2+ concentration ([Ca2+]i) in beta-cells exposed to 20 mM glucose ...
P E, Lund, E, Gylfe
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Characterization, Guanosine 5′-O-(3-Thiotriphosphate) Modulation, Daily Variation, and Localization of Melatonin-Binding Sites in the Catfish (Silurus asotus) Brain

General and Comparative Endocrinology, 1997
Characteristics, guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) modulation, daily variation, and localization of melatonin-binding sites in the brain of a nocturnal teleost, the catfish Silurus asotus, were studied by radioreceptor assay using 2-[125I]iodomelatonin as the radioligand.
M, Iigo, F J, Sánchez-Vázquez, M, Hara, R, Ohtani-Kaneko, K, Hirata, H, Shinohara, M, Tabata, K, Aida   +7 more
openaire   +2 more sources

Cell-free translocation of recombinant p47-phox, a component of the neutrophil NADPH oxidase: effects of guanosine 5'-O-(3-thiotriphosphate), diacylglycerol, and an anionic amphiphile

Biochemistry, 1992
We reported previously that diacylglycerol (diC8) and GTP gamma S synergize with an anionic amphiphile such as sodium dodecyl sulfate (SDS) to produce high rates of superoxide generation in a cell-free system consisting of neutrophil plasma membrane plus cytosol [Burnham, D. N., Uhlinger, D. J., & Lambeth, J. D. (1990) J. Biol. Chem. 265, 17550-17559].
S R, Tyagi, N, Neckelmann, D J, Uhlinger, D N, Burnham, J D, Lambeth   +4 more
openaire   +2 more sources

Combination of Arachidonic Acid and Guanosine 5′-O-(3-Thiotriphosphate) Induces Translocation of rac p21s to Membrane and Activation of NADPH Oxidase in a Cell-Free System

Biochemical and Biophysical Research Communications, 1993
The superoxide-generating NADPH oxidase system in phagocytes consists of membrane-associated cytochrome b558 and three cytosolic components named p67-phox, p47-phox, and rac p21s. In a cell-free system consisting of membrane and cytosol, the oxidase can be activated with guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and an unsaturated fatty acid ...
T, Sawai, M, Asada, H, Nunoi, I, Matsuda, S, Ando, T, Sasaki, K, Kaibuchi, Y, Takai, K, Katayama   +8 more
openaire   +2 more sources

Catecholamine-stimulated guanosine 5'-O-(3-thiotriphosphate) binding to the stimulatory GTP-binding protein of adenylate cyclase: kinetic analysis in reconstituted phospholipid vesicles

Biochemistry, 1984
The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with respect to the stimulation of this ...
T, Asano, E M, Ross
openaire   +2 more sources

Effect of Guanosine 5′‐O‐(3‐Thiotriphosphate) and Calcium on γ‐Aminobutyric Acid_B Binding as a Function of Postnatal Development

Journal of Neurochemistry, 1989
Abstract: We have examined the development of γ‐aminobutyric acidB (GABAB) receptors in rat cerebrum using a binding assay that has achieved specific binding levels of ∼50% with the GABAB ligand (–)‐[3H]baclofen. As early as postnatal day 1, GABAB receptors are present and are linked to both calcium‐ and guanosine triphosphate‐binding protein (G ...
M I, al-Dahan, R H, Thalmann
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Guanosine 5′-O-(3-Thiotriphosphate)-induced O−2Generation in Permeabilized Neutrophils Requires Protein Kinase C and Phospholipase C but Not Tyrosine Kinase or Phospholipase D

Archives of Biochemistry and Biophysics, 1999
Guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) induces respiratory burst (O-2 generation) in permeabilized human neutrophils. The signal pathway from GTPgammaS to the enzyme responsible for O-2 generation (NADPH oxidase) is not well defined. To elucidate the signaling pathway activated by GTPgammaS, we used selective inhibitors to test for the ...
M, Tamura, K, Yoshida, K, Kataoka
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Plasma membrane associated phospholipase C from human platelets: synergistic stimulation of phosphatidylinositol 4,5-bisphosphate hydrolysis by thrombin and guanosine 5'-O-(3-thiotriphosphate)

Biochemistry, 1989
The effects of thrombin and GTP gamma S on the hydrolysis of phosphoinositides by membrane-associated phospholipase C (PLC) from human platelets were examined with endogenous [3H]inositol-labeled membranes or with lipid vesicles containing either [3H]phosphatidylinositol or [3H]phosphatidylinositol 4,5-bisphosphate.
J J, Baldassare, P A, Henderson, G J, Fisher   +2 more
openaire   +2 more sources