Results 131 to 139 of about 2,776 (139)

Guanosine 5′-O-(3-thiotriphosphate) causes endothelium-dependent, pertussis toxin-sensitive relaxations in porcine coronary arteries

Biochemical and Biophysical Research Communications, 1992
To determine whether direct stimulation of endothelial G-proteins causes relaxations of the underlying vascular smooth muscle, the effects of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and sodium fluoride were studied in porcine coronary arteries and endothelial cells.
Toshiro Shibano, Juan Codina, Lutz Birnbaumer, Paul M. Vanhoutte   +3 more
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Cell-free translocation of recombinant p47-phox, a component of the neutrophil NADPH oxidase: effects of guanosine 5'-O-(3-thiotriphosphate), diacylglycerol, and an anionic amphiphile

Biochemistry, 1992
We reported previously that diacylglycerol (diC8) and GTP gamma S synergize with an anionic amphiphile such as sodium dodecyl sulfate (SDS) to produce high rates of superoxide generation in a cell-free system consisting of neutrophil plasma membrane plus cytosol [Burnham, D. N., Uhlinger, D. J., & Lambeth, J. D. (1990) J. Biol. Chem. 265, 17550-17559].
Shiv Raj Tyagi, N. Neckelmann, David J. Uhlinger, D N Burnham, J. David Lambeth   +4 more
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Catecholamine-stimulated guanosine 5'-O-(3-thiotriphosphate) binding to the stimulatory GTP-binding protein of adenylate cyclase: kinetic analysis in reconstituted phospholipid vesicles

Biochemistry, 1984
The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with respect to the stimulation of this ...
Tomiko Asano, Elliott M. Ross
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Cholesterol-Dependent Association of Caveolin-1 with the Transducin α Subunit in Bovine Photoreceptor Rod Outer Segments: Disruption by Cyclodextrin and Guanosine 5‘-O-(3-Thiotriphosphate)

Biochemistry, 2003
Evidence suggests that caveolins, 21-24 kDa cholesterol-binding proteins that generally reside in specialized detergent-resistant membrane microdomains, act as signaling scaffolds. Detergent-resistant membranes isolated from rod outer segments (ROS) have been previously shown to contain the photoreceptor G-protein, transducin.
Michael H. Elliott, Steven J. Fliesler, Abboud J. Ghalayini   +2 more
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Differential effects of guanosine 5′-O-(3-thiotriphosphate) (GTPγS) on the 2-[125I]iodomelatonin binding sites in the chicken bursa of Fabricius and spleen

Neuroscience Letters, 1994
Effects of 10 and 50 mumol/l guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) on 2-[125I]iodomelatonin binding in the chicken bursa of Fabricius and spleen were tested. In the chicken bursa of Fabricius, GTP gamma S increased the Kd of 2-[125I]iodomelatonin binding sites without affecting the Bmax value.
A.M.S. Poon, S.F. Pang
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Role of G-protein availability in differential signaling by alpha 2-adrenoceptors 1 1Abbreviations: α2-AR, α2-adrenergic receptor; AC, adenylyl cyclase; GTPγS, guanosine 5′-O-(3-thiotriphosphate); IBMX, 3-isobutyl-1-methyl-xanthine; UK14,304, 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine.

Biochemical Pharmacology, 2001
The impact of G-protein expression on the coupling specificity of the human alpha(2B)-adrenergic receptor (alpha(2B)-AR) was studied in Sf9 cells. The alpha(2B)-AR was shown to activate both coexpressed G(s)- and G(i)-proteins in a [(35)S]GTPgammaS binding assay.
Johnny Näsman, Jyrki P. Kukkonen, Sylwia Ammoun, Karl E.O. Åkerman   +3 more
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Guanosine 5′-O-(3-thiotriphosphate) and thyroliberin stimulate inositol phosphate production in GH3 cell homogenates [PDF]

Biochemical Society Transactions, 1986
Lawrence A. Quilliam, J. G. Baird, P.R.M. Dobson, B. L. Brown   +3 more
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Rapid binding of guanosine 5'-0-(3-thiotriphosphate) to an apparent complex of .beta.-adrenergic receptor and the GTP-binding regulatory protein Gs

Biochemistry, 1988
When reconstituted phospholipid vesicles that contain purified beta-adrenergic receptors and the GTP-binding regulatory protein Gs were preincubated with agonist before the addition of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), the typical receptor-stimulated GTP gamma S binding reaction was preceded by an even more rapid burst of GTP gamma S ...
David C. May, Elliott M. Ross
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Stimulation of Guanosine 5′‐O‐(3‐[³⁵S]Thiotriphosphate) Binding by Cholinergic Muscarinic Receptors in Membranes of Rat Olfactory Bulb

, 1996
Maria C. Olianas, Pierluigi Onali
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