Results 11 to 20 of about 2,776 (139)

Parthenogenetic Activation of Porcine Oocytes with Guanosine-5′-O-(3′-Thiotriphosphate)1 [PDF]

Biology of Reproduction, 1995
A possible mechanism by which sperm activate oocytes may involve a G protein-mediated signal-transducing pathway. Artificial stimulation of G proteins leads to events very similar to those at fertilization in oocytes of several species. Here we report the effects of activation of a G protein with guanosine-5'-O-(3'-thiotriphosphate) (GTP-gamma-S), a ...
Zoltán Macháty, Mario A. Mayes, Randall S. Prather   +2 more
openalex   +4 more sources

Inositol 1,4,5-trisphosphate- and guanosine 5'-O-(3-thiotriphosphate)-induced Ca2+ release in cultured airway smooth muscle. [PDF]

Br J Pharmacol, 1991
The interaction between inositol 1,4,5‐trisphosphate (InsP3) and guanosine 5′‐O‐(3‐thio triphosphate) (GTPγS) releasable calcium (Ca2+) pools was examined using 45Ca effluxes in permeabilized cultured airway smooth muscle cells from rabbit trachea. Addition of InsP3 or GTPγS caused a concentration‐dependent release of intracellular Ca2+.
Chopra LC, Twort CH, Cameron IR, Ward JP.   +3 more
europepmc   +5 more sources

Carbachol in the presence of guanosine 5′-O-(3-thiotriphosphate) stimulates the breakdown of exogenous phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol by rat brain membranes

Journal of Biological Chemistry, 1989
The breakdown of exogenously added [3H]inositol-labeled phosphoinositides by rat brain cortical membranes was stimulated by the muscarinic cholinergic agonist carbachol. The stimulation required the presence of guanine nucleotide. Optimal conditions were similar to those described for guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) + carbachol ...
Enrique Claro, M A Wallace, H M Lee, John N. Fain   +3 more
openalex   +4 more sources

Guanosine 5'-O-(3-thiotriphosphate) as an analog of GTP in protein biosynthesis. The effects of temperature and polycations on the accuracy of initial recognition of aminoacyl-tRNA ternary complexes by ribosomes.

Journal of Biological Chemistry, 1986
Guanosine 5'-O-(3-thio)triphosphate (GTP gamma S) is a good analog of GTP in the reactions leading to the formation of a peptide bond in protein biosynthesis. It forms binary and ternary complexes with elongation factor Tu (EF-Tu), and with EF-Tu and aminoacyl-tRNA (aa-tRNA). In addition, it stimulates aa-tRNA binding to ribosomes. Although GTP gamma S
Amr M. Karim, Richard Thompson
openalex   +4 more sources

Phospholipase D activation in a cell-free system from human neutrophils by phorbol 12-myristate 13-acetate and guanosine 5'-O-(3-thiotriphosphate). Activation is calcium dependent and requires protein factors in both the plasma membrane and cytosol.

Journal of Biological Chemistry, 1991
Receptor-linked activation of phospholipase D has been demonstrated recently in a variety of intact cell types including granulocytes, but little is known about the enzyme, its cofactor requirements, and regulation. Using [3H]alkyllysophosphatidylcholine to prelable an endogenous phosphatidylcholine substrate pool in conjunction with ...
Susan C. Olson, Edward P. Bowman, J. David Lambeth   +2 more
openalex   +4 more sources

Evidence that a beta-adrenergic receptor-associated guanine nucleotide regulatory protein conveys guanosine 5'-O-(3-thiotriphosphate)- dependent adenylate cyclase activity.

Journal of Biological Chemistry, 1981
The guanine nucleotide regulatory protein component (N) of the frog erythrocyte membrane adenylate cyclase system appears to form a stable complex with the beta-adrenergic receptor (R) in the presence of agonist (H). This agonist-promoted ternary complex HRN can be solubilized with Lubrol.
Jeffrey M. Stadel, Robert Shorr, L E Limbird, Robert J. Lefkowitz   +3 more
openalex   +4 more sources

Guanosine 5'-O-(3-thiotriphosphate) and guanosine 5'-O-(2-thiodiphosphate) activate G proteins and potentiate fibroblast growth factor-induced DNA synthesis in hamster fibroblasts.

Journal of Biological Chemistry, 1990
L'ajout de guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) à des fibroblastes pulmonaires intacts de hamster chinois (CCL39) dépolarisés par des concentrations élevées de K+ entraîne l'activation de la phospholipase C spécifique du phosphoinositide (PLC) (à des concentrations de GTP gamma S supérieures à 0,1 mM), l'inhibition de l'adénylate cyclase ...
S Paris, Jacques Pouysségur
openalex   +4 more sources

Guanosine 5'-O-(3-thiotriphosphate) reduces ADP-ribosylation of the inhibitory guanine nucleotide-binding regulatory protein of adenylyl cyclase (Ni) by pertussis toxin without causing dissociation of the subunits of Ni. Evidence of existence of heterotrimeric pt+ and pt- conformations of Ni.

Journal of Biological Chemistry, 1987
The effect of the addition of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), the GTP analog which activates the inhibitory guanine nucleotide-binding regulatory protein of adenylyl cyclase (Ni), on the pertussis toxin-mediated ADP-ribosylation reaction was studied in detail.
Rafael Mattera, Juan Codina, Ronald D. Sekura, Lutz Birnbaumer   +3 more
openalex   +4 more sources

Muscarinic acetylcholine receptor‐stimulated binding of guanosine 5′‐O‐(3‐thiotriphosphate) to guanine‐nucleotide‐binding proteins in cardiac membranes [PDF]

European Journal of Biochemistry, 1989
Receptor‐regulated binding of the labeled GTP analog, guanosine 5′‐O‐(3‐thiotriphosphate) ([35S]GTP[S]), to guanine‐nucleotide‐binding proteins (G‐proteins) was studied in porcine atrial membranes enriched in muscarinic acetylcholine (mACh) receptors. Binding of [35S]GTP[S] to the membranes was not or only slightly affected by the cholinergic agonist ...
Gerhard Hilf, Peter Gierschik, Karl H. Jakobs   +2 more
openalex   +4 more sources

Guanosine 5´-O-(3-Thiotriphosphate) and Cations Regulate Melatonin Receptors, and Melatonin Inhibits Cyclic AMP Production in the Spinal Cord

Neurosignals, 1997
Effects of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and cations on 2-[125I]iodomelatonin binding were investigated in membrane preparations of the chicken spinal cord. At concentrations of 10 and 50 mumol/l, GTP gamma S dose-dependently increased (p < 0.05) the equilibrium dissociation constant (Kd) and depressed (p < 0.05) the maximum number ...
Qi Wan, Minjie Liao, C.S. Pang, Shanshan Pang, Gregory M. Brown   +4 more
openalex   +4 more sources