Results 11 to 20 of about 6,222 (140)

Guanosine-5'-O-(3-thiotriphosphate) modifies kinetics of voltage-dependent calcium current in chick sensory neurons. [PDF]

Biophys J, 1989
Internal perfusion with the G-protein activator guanosine-5'-O-(3-thiotriphosphate) (GTP-gamma S) mimics the effect of noradrenaline and dopamine on the voltage-dependent calcium current in chick dorsal root ganglion (DRG) cells. With 100 microM GTP-gamma S in the pipette, the current at +10 mV was depressed by approximately 50%, with a 10-fold ...
Marchetti C, Robello M.
europepmc   +5 more sources

Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils. [PDF]

J Exp Med, 1990
The mechanism of eosinophil secretion was studied in guinea pig eosinophils by measuring release of hexosaminidase from cell suspensions (greater than 98% pure) permeabilized with streptolysin-O and by whole-cell patch-clamp capacitance measurements. It is shown that release of eosinophil granule components occurs by an exocytotic mechanism in which ...
Nüsse O, Lindau M, Cromwell O, Kay AB, Gomperts BD.   +4 more
europepmc   +6 more sources

Ligand-Independent GLUT4 Translocation Induced by Guanosine 5′-O-(3-Thiotriphosphate) Involves Tyrosine Phosphorylation* [PDF]

Endocrinology, 1998
AbstractTo delineate the signaling pathway leading to glucose transport protein (GLUT4) translocation, we examined the effect of microinjection of the nonhydrolyzable GTP analog, guanosine 5′-O-(3-thiotriphosphate) (GTPγS), into 3T3-L1 adipocytes. Thirty minutes after the injection of 5 mm GTPγS, 40% of injected cells displayed surface GLUT4 staining ...
T, Haruta, A J, Morris, P, Vollenweider, J G, Nelson, D W, Rose, M, Mueckler, J M, Olefsky   +6 more
core   +4 more sources

Biochemical Engineering Perspective on cGAS: From Enzyme Discovery to Potential Industrial Application. [PDF]

Chembiochem
This review examines the structural and enzymatic properties of cyclic GMP‐AMP synthase, including substrate promiscuity, homologs, and engineered variants. In addition, it evaluates potential expression systems for large‐scale enzyme production and analyzes reaction and purification strategies for the synthesis of cyclic dinucleotides to connect ...
Fataeri M, Rosenthal K.
europepmc   +2 more sources

Inositol 1,4,5-trisphosphate- and guanosine 5'-O-(3-thiotriphosphate)-induced Ca2+ release in cultured airway smooth muscle. [PDF]

Br J Pharmacol, 1991
The interaction between inositol 1,4,5‐trisphosphate (InsP3) and guanosine 5′‐O‐(3‐thio triphosphate) (GTPγS) releasable calcium (Ca2+) pools was examined using 45Ca effluxes in permeabilized cultured airway smooth muscle cells from rabbit trachea. Addition of InsP3 or GTPγS caused a concentration‐dependent release of intracellular Ca2+.
Chopra LC, Twort CH, Cameron IR, Ward JP.   +3 more
europepmc   +4 more sources

Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5'-O-(3-thiotriphosphate). [PDF]

Mol Cell Biol, 1987
Rat pheochromocytoma (PC12) cells differentiate to neuronal cells in response to nerve growth factor. It has been shown that microinjection of oncogenic but not proto-oncogenic p21 protein induces morphological differentiation in PC12 cells (D. Bar-Sagi and J. R. Feramisco, Cell 42:841-848, 1985).
Satoh T, Nakamura S, Kaziro Y.
europepmc   +4 more sources

Parthenogenetic activation of porcine oocytes with guanosine-5'-O-(3'-thiotriphosphate). [PDF]

Biology of reproduction, 1995
A possible mechanism by which sperm activate oocytes may involve a G protein-mediated signal-transducing pathway. Artificial stimulation of G proteins leads to events very similar to those at fertilization in oocytes of several species. Here we report the effects of activation of a G protein with guanosine-5'-O-(3'-thiotriphosphate) (GTP-gamma-S), a ...
Z, Macháty, M A, Mayes, R S, Prather
openaire   +2 more sources

Lack of the Transient Receptor Potential Vanilloid 1 Shifts Cannabinoid-Dependent Excitatory Synaptic Plasticity in the Dentate Gyrus of the Mouse Brain Hippocampus

Frontiers in Neuroanatomy, 2021
The transient receptor potential vanilloid 1 (TRPV1) participates in synaptic functions in the brain. In the dentate gyrus, post-synaptic TRPV1 in the granule cell (GC) dendritic spines mediates a type of long-term depression (LTD) of the excitatory ...
Jon Egaña-Huguet, Jon Egaña-Huguet, Miquel Saumell-Esnaola, Miquel Saumell-Esnaola, Svein Achicallende, Svein Achicallende, Edgar Soria-Gomez, Edgar Soria-Gomez, Edgar Soria-Gomez, Itziar Bonilla-Del Río, Itziar Bonilla-Del Río, Gontzal García del Caño, Gontzal García del Caño, Sergio Barrondo, Sergio Barrondo, Joan Sallés, Joan Sallés, Inmaculada Gerrikagoitia, Inmaculada Gerrikagoitia, Nagore Puente, Nagore Puente, Izaskun Elezgarai, Izaskun Elezgarai, Pedro Grandes, Pedro Grandes, Pedro Grandes   +25 more
doaj   +1 more source

Functional Interaction Between Caveolin 1 and LRRC8-Mediated Volume-Regulated Anion Channel

Frontiers in Physiology, 2021
Volume-regulated anion channel (VRAC), constituted by leucine-rich repeat-containing 8 (LRRC8) heteromers, is crucial for volume homeostasis in vertebrate cells.
Mikel Rezola, Aida Castellanos, Xavier Gasull, Xavier Gasull, Núria Comes, Núria Comes   +5 more
doaj   +1 more source

3‐[3‐(Phenalkylamino)cyclohexyl]phenols: Synthesis, biological activity, and in silico investigation of a naltrexone‐derived novel class of MOR‐antagonists

Archiv der Pharmazie, Volume 356, Issue 1, January 2023., 2023
Based on a simplified version of the morphinan scaffold, 3‐[3‐(phenalkylamino)cyclohexyl]phenol analogues were designed, synthesized and evaluated for their µ‐opioid receptor (MOR) antagonist activity in vitro and in silico. Docking studies indicate a peculiar combination of C‐1 stereochemistry and N‐substitutions as feasibly essential for MOR‐ligand ...
Graziella Tocco, Antonio Laus, Maksims Vanejevs, Anastasija Ture, Rafaela Mostallino, Nicholas Pintori, Maria Antonietta De Luca, M. Paola Castelli, Gaetano Di Chiara   +8 more
wiley   +1 more source