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Accumulation of Guanosine Tetraphosphate in T7 Bacteriophage-Infected Escherichia coli
Journal of Bacteriology, 1973Accumulation of guanosine tetraphosphate (ppGpp) in Escherichia coli strain AS19 valS , carrying a temperature-sensitive valyl-transfer ribonucleic acid synthetase and infected with bacteriophage T7, was studied. Valine starvation was achieved by culturing this strain at 42 C.
J D, Friesen, N, Fiil
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Altered metabolism of the guanosine tetraphosphate, ppGpp, in mutants of E. coli
Cell, 1974Abstract The metabolism of ppGpp is altered in commonly used mutants of E. coli. While retaining their ability to increase rapidly the intracellular level of the nucleotide during amino acid or carbon source deprivation, they are impaired in their ability to reduce an elevated level.
Gudrun Stamminger, Robert A. Lazzarini
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Phosphoglycerate kinase from yeast synthesizes guanosine 5'-tetraphosphate.
Biochemistry international, 1990Yeast phosphoglycerate kinase (PGK; EC 2.7.2.3) synthesizes adenosine 5'-tetraphosphate (ppppA) from ATP and 1,3-bisphosphoglycerate. Using an HPLC assay, we have shown the synthesis of guanosine 5'-tetraphosphate (ppppG; 0.17 nmol.min-1.PGK unit-1) characterized by its u.v. spectrum, HPLC behavior, and enzymatic digestions. That the synthesis of ppppG
M, García-Díaz +4 more
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La guanosine tetraphosphate ou ppGpp a été initialement découverte chez la bactérie Escherichia coli. Il s'agit de la molécule clé qui permet la régulation de la croissance et promeut l'acclimatation au stress des procaryotes.
Shanna Romand
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
Abstract Accumulation of guanosine 5′-diphosphate-2′(or 3′)-diphosphate was not detected in Neurospora crassa during a shift-down transition of growth, which restricts RNA synthesis. This is non-supportive evidence for its role as a mediator in the inhibition of stable RNA synthesis in eukaryotic cells.
F. A. Alberghina +3 more
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Abstract Accumulation of guanosine 5′-diphosphate-2′(or 3′)-diphosphate was not detected in Neurospora crassa during a shift-down transition of growth, which restricts RNA synthesis. This is non-supportive evidence for its role as a mediator in the inhibition of stable RNA synthesis in eukaryotic cells.
F. A. Alberghina +3 more
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Inapparent correlation between guanosine tetraphosphate levels and RNA contents in Escherichia coli
Biochemical and Biophysical Research Communications, 1974Summary We have reexamined the intracellular levels of guanosine 5′-diphosphate 3′-diphosphate in a prototrophic stringent strain of Escherichia coli during balanced growth at eight different growth rates. No regular inverse relationship between the basal levels of this nucleotide and the RNA content per unit DNA was observed. Thus, it is concluded
S R, Khan, H, Yamazaki
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Archives of Microbiology, 1981
The effect of nitrate deprivation on cell growth and nucleotide level was studied in Anacystis nidulans. A 10-fold reduction in nitrate level resulted in a drastic slowdown of growth. Upon addition of nitrate to the starving cultures, after a lag period, the cells resumed growth.
G M, Friga, G, Borbély, G L, Farkas
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The effect of nitrate deprivation on cell growth and nucleotide level was studied in Anacystis nidulans. A 10-fold reduction in nitrate level resulted in a drastic slowdown of growth. Upon addition of nitrate to the starving cultures, after a lag period, the cells resumed growth.
G M, Friga, G, Borbély, G L, Farkas
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Journal of Biotechnology, 1995
A rapid transient increase of guanosine 3'-diphosphate 5'-diphosphate (ppGpp) in Escherichia coli was found in response to short-term glucose fluctuations that may occur in large-scale fed-batch cultivations. The concentration of ppGpp was measured in laboratory-scale glucose limited fed-batch cultivations.
P, Neubauer +4 more
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A rapid transient increase of guanosine 3'-diphosphate 5'-diphosphate (ppGpp) in Escherichia coli was found in response to short-term glucose fluctuations that may occur in large-scale fed-batch cultivations. The concentration of ppGpp was measured in laboratory-scale glucose limited fed-batch cultivations.
P, Neubauer +4 more
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Analytical Biochemistry, 1988
Commercial samples of GTP and guanosine 5'-tetraphosphate were analyzed, with or without previous treatment with alkaline phosphatase, by high-pressure liquid chromatography on a Hypersil ODS column. They showed the presence of diguanosine 5',5"'-Pl,Pn-tri, tetra-, and pentaphosphates in varying amounts depending on the sample, but usually in ...
F, Buitrago, J, Canales, A, Sillero
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Commercial samples of GTP and guanosine 5'-tetraphosphate were analyzed, with or without previous treatment with alkaline phosphatase, by high-pressure liquid chromatography on a Hypersil ODS column. They showed the presence of diguanosine 5',5"'-Pl,Pn-tri, tetra-, and pentaphosphates in varying amounts depending on the sample, but usually in ...
F, Buitrago, J, Canales, A, Sillero
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