Results 251 to 260 of about 85,574 (298)
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Analytical Biochemistry, 1989
A procedure for enzymatic production of dihydroneopterin triphosphate is described that allows GTP cyclohydrolase I to be reused repetitively. The reaction takes place in an ultrafiltration cell, and the product is collected in the filtrate, whereas the enzyme remains in the cell to be reused with additional substrate. This is repeated until the enzyme
J, Ferre, E W, Naylor, K B, Jacobson
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A procedure for enzymatic production of dihydroneopterin triphosphate is described that allows GTP cyclohydrolase I to be reused repetitively. The reaction takes place in an ultrafiltration cell, and the product is collected in the filtrate, whereas the enzyme remains in the cell to be reused with additional substrate. This is repeated until the enzyme
J, Ferre, E W, Naylor, K B, Jacobson
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Synthesis of Guanosine-Containing Dinucleoside Triphosphates
Advanced Materials Research, 2014Dinucleoside polyphosphates have potential medicinal value and are of substantial interest to both synthetic and medicinal chemists. A practical method for the synthesis of guanosine-containing asymmetrical dinucleoside triphosphates (Gp3C and Gp3U) with 4,5-dicyanoimidazole as activator has been developed.
Yue Hai You, Shan Shan Gong, Qi Sun
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The effect of guanosine 5′-triphosphate analogues on protein synthesis
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971Abstract Four GTP analogues, 6-mercaptoguanosine 5′-triphosphate (I), N2-methylguanosine 5′-triphosphate (II), N2-dimethylguanosine 5′-triphosphate (III) and 8-bromoguanosine 5′-triphosphate, (IV) were synthesized chemically and tested for their ability to replace GTP in the cell-free amino acid incorporation system of Escherichia coli.
H, Uno, S, Oyabu, E, Otsuka, M, Ikehara
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Evidence for Guanosine Triphosphate—Binding Proteins in Trypanosoma cruzi
The American Journal of Tropical Medicine and Hygiene, 1994The transformation of the parasite Trypanosoma cruzi from the blood-borne trypomastigote to the intracellular amastigote constitutes a key clinical feature in the pathophysiology of Chagas' disease. That this transition occurs without change in the integrity of the plasma membrane of the parasite suggests the presence of biochemical structures, i.e ...
H S, Oz +5 more
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Direct photoaffinity labeling of tubulin with guanosine 5'-triphosphate
Biochemistry, 1985Irradiation of tubulin in the presence of [3H]GTP or [3H]GDP at 254 nm led to the covalent incorporation of nucleotide into the protein. The specific nature of the labeling was shown in the following manner: with tubulin depleted of exchangeable nucleotide, the amount of labeling increased to a plateau value as the [3H]GTP concentration was increased ...
J P, Nath, G R, Eagle, R H, Himes
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Analytical Chemistry
Guanosine triphosphate (GTP)-binding proteins function as molecular switches in cell signaling, playing critical roles in various biological pathways. Their dysregulation is associated with the causes and progression of many diseases. Systematic analysis of GTP-binding proteins would facilitate studies of related signaling pathways and drugs ...
Can Gao +8 more
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Guanosine triphosphate (GTP)-binding proteins function as molecular switches in cell signaling, playing critical roles in various biological pathways. Their dysregulation is associated with the causes and progression of many diseases. Systematic analysis of GTP-binding proteins would facilitate studies of related signaling pathways and drugs ...
Can Gao +8 more
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On the mechanism of guanosine triphosphate hydrolysis in ras p21 proteins
Biochemistry, 1992The residue Gln61 is assumed to play a major role in the mechanism of ras p21, and mutations of this residue are often found in human tumors. Such mutations lead to a major reduction in the rate of GTP hydrolysis by the complex of ras p21 and the GTPase activating protein (GAP) and lock the protein in a growth-promoting state.
R, Langen, T, Schweins, A, Warshel
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Adenosine Triphosphate and Guanosine Triphosphate Determinations in Intertidal Sediments
1979Measurements were made of the spatial distributions of adenosine triphosphate (ATP) on a variety of sandy beaches. Samples were collected during ebb flow and extracted in the field using cold sulfuric acid and ethylenediamine tetraacetate (EDTA); the neutralized extracts were immediately frozen for subsequent analyses.
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[37] Guanosine triphosphate-8-formylhydrolase
1975Publisher Summary This chapter discusses the assay and purification procedure of guanosine triphosphate-8-formylhydrolase. The assay method is based on two procedures. The first method involves the release of 14 C-labeled formic acid from carbon-8 of the imidazole ring of [8- 14 C]GTP. The formic acid is oxidized to carbon dioxide. The second method
E.F. Elstner, R.J. Suhadolnik
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Biochemistry, 1986
Using highly purified calf brain tubulin bearing [8-14C]guanosine 5'-diphosphate (GDP) in the exchangeable nucleotide site and heat-treated microtubule-associated proteins (both components containing negligible amounts of nucleoside diphosphate kinase and nonspecific phosphatase activities), we have found that a significant proportion of exchangeable ...
E, Hamel, J K, Batra, C M, Lin
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Using highly purified calf brain tubulin bearing [8-14C]guanosine 5'-diphosphate (GDP) in the exchangeable nucleotide site and heat-treated microtubule-associated proteins (both components containing negligible amounts of nucleoside diphosphate kinase and nonspecific phosphatase activities), we have found that a significant proportion of exchangeable ...
E, Hamel, J K, Batra, C M, Lin
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