Results 231 to 240 of about 2,639,376 (307)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of <i>Geobacillus stearothermophilus</i> Cas9. [PDF]

Elife
Belato HB, Knight AL, D'Ordine AM, Pindi C, Fan Z, Luo J, Palermo G, Jogl G, Lisi GP.   +8 more
europepmc   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands. [PDF]

Nucleic Acids Res
Miceli F, Bracaglia S, Sorrentino D, Porchetta A, Ranallo S, Ricci F.   +5 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Deep learning enhancing guide RNA design for CRISPR/Cas12a-based diagnostics. [PDF]

Imeta
Huang B, Guo L, Yin H, Wu Y, Zeng Z, Xu S, Lou Y, Ai Z, Zhang W, Kan X, Yu Q, Du S, Li C, Wu L, Huang X, Wang S, Wang X.   +16 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Single-guide RNA Cas9 and enhanced-deletion Cas9 rescue a recurrent <i>USH2A</i>-related splicing defect. [PDF]

Mol Ther Nucleic Acids
De Angeli P, Spaag S, Shliaga S, Flores-Tufiño A, Ritter M, Nasri M, Stingl K, Kühlewein L, Wissinger B, Kohl S.   +9 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Model-directed generation of artificial CRISPR-Cas13a guide RNA sequences improves nucleic acid detection. [PDF]

Nat Biotechnol
Mantena S, Pillai PP, Petros BA, Welch NL, Myhrvold C, Sabeti PC, Metsky HC.   +6 more
europepmc   +1 more source