Results 251 to 260 of about 2,639,376 (307)

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Guide RNA library representation results in gene essentiality prediction bias in genome-wide CRISPR screens

Metz P, Alves-Vasconcelos S, Wallbank R, Riepsaame J, Hassan AB.   +4 more
europepmc   +1 more source

Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA. [PDF]

Nat Biotechnol
Reautschnig P, Fruhner C, Wahn N, Wiegand CP, Kragness S, Yung JF, Hofacker DT, Fisk J, Eidelman M, Waffenschmidt N, Feige M, Pfeiffer LS, Schulz AE, Füll Y, Levanon EY, Mandel G, Stafforst T.   +16 more
europepmc   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex. [PDF]

Mol Cell
Wang PY, Bartel DP.
europepmc   +1 more source

A benchmark comparison of CRISPRn guide-RNA design algorithms and generation of small single and dual-targeting libraries to boost screening efficiency. [PDF]

BMC Genomics
Lukasiak S, Kalinka A, Gupta N, Papadopoulos A, Saeed K, McDermott U, Hannon GJ, Ross-Thriepland D, Walter D.   +8 more
europepmc   +1 more source

A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo. [PDF]

Mol Ther
Li YJ, Chien SH, Huang R, Herrmann A, Zhao Q, Li PC, Zhang C, Martincuks A, Santiago NL, Zong K, Swiderski P, Okimoto RA, Song M, Rodriguez L, Forman SJ, Wang X, Yu H.   +16 more
europepmc   +1 more source

CRISPR-Cas guide RNA indel analysis using CRISPResso2 with Nanopore sequencing data. [PDF]

BMC Res Notes
McFarlane GR, Polanco JVC, Bogema D.
europepmc   +1 more source

CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris. [PDF]

Synth Syst Biotechnol
Chen K, Maimaitirexiati G, Zhang Q, Li Y, Liu X, Tang H, Gao X, Wang B, Yu T, Guo S.   +9 more
europepmc   +1 more source

Re-engineered guide RNA enables DNA loops and contacts modulating repression in E. coli. [PDF]

Nucleic Acids Res
Yang Y, Rocamonde-Lago I, Shen B, Berzina I, Zipf J, Högberg B.   +5 more
europepmc   +1 more source