Results 261 to 270 of about 323,472 (305)

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

SynDesign: web-based prime editing guide RNA design and evaluation tool for saturation genome editing. [PDF]

Nucleic Acids Res
Park J, Yu G, Seo SY, Yang J, Kim HH.
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Cas9/guide RNA-based gene-drive dynamics following introduction and introgression into diverse anopheline mosquito genetic backgrounds. [PDF]

BMC Genomics
Tushar T, Pham TB, Parker K, Crepeau M, Lanzaro GC, James AA, Carballar-Lejarazú R.   +6 more
europepmc   +1 more source

Compact CRISPR genetic screens enabled by improved guide RNA library cloning. [PDF]

Genome Biol
Heo SJ, Enriquez LD, Federman S, Chang AY, Mace R, Shevade K, Nguyen P, Litterman AJ, Shafer S, Przybyla L, Chow ED.   +10 more
europepmc   +1 more source

CRISPR/Pepper-tDeg: A Live Imaging System Enables Non-Repetitive Genomic Locus Analysis with One Single-Guide RNA. [PDF]

Adv Sci (Weinh)
Chen M, Huang X, Shi Y, Wang W, Huang Z, Tong Y, Zou X, Xu Y, Dai Z.   +8 more
europepmc   +1 more source

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Chimeric DNA–RNA Guide RNA Designs

2020
CRISPR-associated nuclease (Cas) has been widely applied to modify the genomes of various cell types. As RNA-guided endonucleases, Cas enzymes can target different genomic sequences simply by changing the guide sequence of the CRISPR RNA (crRNA) or single guide RNA (sgRNA).
Shuhan, Lu, Ying, Zhang, Hao, Yin
openaire   +2 more sources

RNA: Guiding Gene Silencing

Science, 2001
In diverse organisms, small RNAs derived from cleavage of double-stranded RNA can trigger epigenetic gene silencing in the cytoplasm and at the genome level. Small RNAs can guide posttranscriptional degradation of complementary messenger RNAs and, in plants, transcriptional gene silencing by methylation of homologous DNA sequences.
M, Matzke, A J, Matzke, J M, Kooter
openaire   +2 more sources

RNA-Guided RNA Modifications: Biogenesis, Functions, and Applications

Accounts of Chemical Research, 2023
Post-transcriptional modifications are ubiquitous in both protein-coding and noncoding RNAs (ncRNAs), playing crucial functional roles in diverse biological processes across all kingdoms of life. These RNA modifications can be achieved through two distinct mechanisms: RNA-independent and RNA-guided (also known as RNA-dependent).
Bin Li, Lianghu Qu, Jianhua Yang
openaire   +2 more sources