Results 281 to 290 of about 4,450,920 (398)

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

CRISPR with a Double Mismatch Guide RNA Enhances Detection Sensitivity for Low-Frequency Single-Base EGFR Mutation in Circulating Cell-Free DNA of Lung Cancer Patients. [PDF]

Cancers (Basel)
Been KW, Kang S, Bae T, Hong S, Kim G, Hur JK, Hwang W, Chang B.   +7 more
europepmc   +1 more source

Crystal Structure of a Cbf5-Nop10-Gar1 Complex and Implications in RNA-Guided Pseudouridylation and Dyskeratosis Congenita [PDF]

, 2006
Rumana Rashid, Bo Liang, Daniel L. Baker, Osama Youssef, Yang He, Kathleen R. Phipps, Rebecca M. Terns, Michael P. Terns, Hong Li   +8 more
openalex   +1 more source

A Cas9–guide RNA complex preorganized for target DNA recognition

Science, 2015
F. Jiang, Kaihong Zhou, Linlin Ma, Saskia Gressel, J. Doudna   +4 more
semanticscholar   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of <i>Geobacillus stearothermophilus</i> Cas9. [PDF]

Elife
Belato HB, Knight AL, D'Ordine AM, Pindi C, Fan Z, Luo J, Palermo G, Jogl G, Lisi GP.   +8 more
europepmc   +1 more source

Highly efficient RNA-guided base editing in rabbit

, 2018
Zhiquan Liu, Mao Chen, Siyu Chen, Jichao Deng, Yuning Song, Liangxue Lai, Zhanjun Li   +6 more
openalex   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands. [PDF]

Nucleic Acids Res
Miceli F, Bracaglia S, Sorrentino D, Porchetta A, Ranallo S, Ricci F.   +5 more
europepmc   +1 more source

Reviewer #3 (Public review): Structural and Dynamic Impacts of Single-atom Disruptions to Guide RNA Interactions within the Recognition Lobe of Geobacillus stearothermophilus Cas9

Helen B Belato, Alexa L Knight, Alexandra M D’Ordine, Chinmai Pindi, Zhiqiang Fan, Jinping Luo, Giulia Palermo, Gerwald Jogl, George P Lisi   +8 more
openalex   +1 more source