RNA-programmed genome editing in human cells
eLife, 2013 Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA ...
Martin Jinek +5 more
doaj +1 more source
Global analyses of endonucleolytic cleavage in mammals reveal expanded repertoires of cleavage-inducing small RNAs and their targets. [PDF]
, 2016 In mammals, small RNAs are important players in post-transcriptional gene regulation. While their roles in mRNA destabilization and translational repression are well appreciated, their involvement in endonucleolytic cleavage of target RNAs is poorly ...
Bahn, Jae Hoon +7 more
core +2 more sources
RNA editing: Guided but not templated?
Cell, 1990 Article synthese etabli a partir de quatre publications recentes sur l'«edition RNA», son origine, son mode d ...
Nancy Maizels, Alan M. Weiner
openaire +3 more sources
A Graph Grammar for Modelling RNA Folding [PDF]
EPTCS 231, 2016, pp. 31-41, 2016 We propose a new approach for modelling the process of RNA folding as a graph transformation guided by the global value of free energy. Since the folding process evolves towards a configuration in which the free energy is minimal, the global behaviour resembles the one of a self-adaptive system.
arxiv +1 more source
RNA-Seq optimization with eQTL gold standards. [PDF]
, 2013 BackgroundRNA-Sequencing (RNA-Seq) experiments have been optimized for library preparation, mapping, and gene expression estimation. These methods, however, have revealed weaknesses in the next stages of analysis of differential expression, with results ...
Arking, Dan E +5 more
core +2 more sources
The 5' → 3' exoribonuclease XRN1/Pacman and its functions in cellular processes and development [PDF]
, 2012 XRN1 is a 5' → 3' processive exoribonuclease that degrades mRNAs after they have been decapped. It is highly conserved in all eukaryotes, including homologs in Drosophila melanogaster (Pacman), Caenorhabditis elegans (XRN1), and Saccharomyces cerevisiae (
Jones, Christopher Iain +2 more
core +1 more source
A Comparative Study of the Structural Dynamics of Four Terminal Uridylyl Transferases. [PDF]
, 2017 African trypanosomiasis occurs in 36 countries in sub-Saharan Africa with 10,000 reported cases annually. No definitive remedy is currently available and if left untreated, the disease becomes fatal.
Amaro, Rommie E +2 more
core +1 more source
Cas13b-dependent and Cas13b-independent RNA knockdown of viral sequences in mosquito cells following guide RNA expression [PDF]
, 2020 Aedes aegypti and Aedes albopictus mosquitoes are vectors of the RNA viruses chikungunya (CHIKV) and dengue that currently have no specific therapeutic treatments. The development of new methods to generate virus-refractory mosquitoes would be beneficial.
Alphey, Luke +8 more
core +6 more sources
Viral RNA Interactome: The Ultimate Researcher’s Guide to RNA–Protein Interactions
VirusesRNA molecules in the cell are bound by a multitude of RNA-binding proteins (RBPs) with a variety of regulatory consequences. Often, interactions with these RNA-binding proteins are facilitated by the complex secondary and tertiary structures of RNA ...
Wesley A. Hanson +2 more
doaj +1 more source
Eukaryote-Specific Insertion Elements Control Human ARGONAUTE Slicer Activity
Cell Reports, 2013 We have solved the crystal structure of human ARGONAUTE1 (hAGO1) bound to endogenous 5′-phosphorylated guide RNAs. To identify changes that evolutionarily rendered hAGO1 inactive, we compared our structure with guide-RNA-containing and cleavage-active ...
Kotaro Nakanishi +8 more
doaj +1 more source