Results 51 to 60 of about 4,450,920 (398)
Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. [PDF]
, 2020 Anti-CRISPRs (Acrs) are small proteins that inhibit the RNA-guided DNA targeting activity of CRISPR-Cas enzymes. Encoded by bacteriophage and phage-derived bacterial genes, Acrs prevent CRISPR-mediated inhibition of phage infection and can also block ...
Doudna, Jennifer A +5 more
core +3 more sources
Short RNA guides cleavage by eukaryotic RNase III.
PLoS ONE, 2007 In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution.
Bruno Lamontagne, Sherif Abou Elela
doaj +1 more source
The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]
, 1992 The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert +3 more
core +1 more source
RNA processing: Pocket guides to ribosomal RNA [PDF]
Current Biology, 1997 The functional role of a recently identified class of small nucleolar (sno) RNAs has been elucidated: the 'box H/ACA' snoRNAs act as guide RNAs, specifying the position of evolutionarily conserved pseudouridines in ribosomal (r)RNA via an rRNA-snoRNA base-pairing interaction that forms a 'pseudouridine pocket'.
openaire +2 more sources
Polyvalent guide RNAs for CRISPR antivirals
iScience, 2022 CRISPR effector Cas13 recognizes and degrades RNA molecules that are complementary to its guide RNA (gRNA) and possesses potential as an antiviral biotechnology because it can degrade viral mRNA and RNA genomes. Because multiplexed targeting is a critical strategy to improve viral suppression, we developed a strategy to design of gRNAs where individual
Rammyani Bagchi +6 more
openaire +3 more sources
Structure of yeast Argonaute with guide RNA [PDF]
, 2012 The RNA-induced silencing complex, comprising Argonaute and guide RNA, mediates RNA interference. Here we report the 3.2 Å crystal structure of Kluyveromyces polysporus Argonaute (KpAGO) fortuitously complexed with guide RNA originating from small-RNA ...
Bartel, David +3 more
core +1 more source
RNA-Seq optimization with eQTL gold standards. [PDF]
, 2013 BackgroundRNA-Sequencing (RNA-Seq) experiments have been optimized for library preparation, mapping, and gene expression estimation. These methods, however, have revealed weaknesses in the next stages of analysis of differential expression, with results ...
Arking, Dan E +5 more
core +2 more sources
A Step-by-Step Guide to Study Protein–RNA Interactions
CHIMIA, 2019 Protein–RNA complex formation is at the center of RNA metabolism and leads to the modulation of protein and RNA functions. We propose here a step-by-step guide to investigate these interactions including the identification of the protein and RNA
Antoine Cléry +3 more
doaj +1 more source
Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. [PDF]
PLoS Genetics, 2013 Discriminating self and non-self is a universal requirement of immune systems. Adaptive immune systems in prokaryotes are centered around repetitive loci called CRISPRs (clustered regularly interspaced short palindromic repeat), into which invader DNA ...
Edze R Westra +6 more
doaj +1 more source
Global analyses of endonucleolytic cleavage in mammals reveal expanded repertoires of cleavage-inducing small RNAs and their targets. [PDF]
, 2016 In mammals, small RNAs are important players in post-transcriptional gene regulation. While their roles in mRNA destabilization and translational repression are well appreciated, their involvement in endonucleolytic cleavage of target RNAs is poorly ...
Bahn, Jae Hoon +7 more
core +2 more sources