Results 61 to 70 of about 570,529 (349)
Biomolecules, 2019 Site-specific 2’-O-ribose methylation is an abundant post-transcriptional modification mediated by small non-coding nuclear RNAs known as box C/D modification guide RNAs.
Svetlana Deryusheva, Joseph G. Gall
doaj +1 more source
Small RNA in the nucleus: the RNA-chromatin ping-pong [PDF]
, 2012 Eukaryotes use several classes of small RNA molecules to guide diverse protein machineries to target messenger RNA. The role of small RNA in post-transcriptional regulation of mRNA stability and translation is now well established.
Aravin, Alexei A. +2 more
core +1 more source
CRISPR/Cas13 Effectors Have Differing Extents of Off-Target Effects That Limit Their Utility in Eukaryotic Cells [PDF]
, 2022 CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for detecting and depleting RNAs of interest. Near perfect complementarity between a target RNA and the Cas13-associated guide RNA is required for activation of Cas13 ...
Ai, Yuxi +2 more
core +2 more sources
Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens
Cell Genomics, 2021 Summary: The recent characterization of RNA-targeting CRISPR nucleases has enabled diverse transcriptome engineering and screening applications that depend crucially on prediction and selection of optimized CRISPR guide RNAs (gRNAs).
Xinyi Guo +6 more
doaj
Conservation of Archaeal C/D Box sRNA-Guided RNA Modifications
Frontiers in Microbiology, 2021 Post-transcriptional modifications fulfill many important roles during ribosomal RNA maturation in all three domains of life. Ribose 2'-O-methylations constitute the most abundant chemical rRNA modification and are, for example, involved in RNA folding ...
Ruth Breuer +2 more
doaj +1 more source
Combining Insertion and Deletion in RNA-editing Preserves Regularity [PDF]
EPTCS 100, 2012, pp. 48-62, 2012 Inspired by RNA-editing as occurs in transcriptional processes in the living cell, we introduce an abstract notion of string adjustment, called guided rewriting. This formalism allows simultaneously inserting and deleting elements. We prove that guided rewriting preserves regularity: for every regular language its closure under guided rewriting is ...
arxiv +1 more source
FEBS Letters, EarlyView.In vivo IL‐10 produced by tissue‐resident tolDC is involved in maintaining/inducing tolerance. Depending on the agent used for ex vivo tolDC generation, cells acquire common features but prime T cells towards anergy, FOXP3+ Tregs, or Tr1 cells according to the levels of IL‐10 produced. Ex vivo‐induced tolDC were administered to patients to re‐establish/
Konstantina Morali +3 more
wiley +1 more source
Using Guided Transfer Learning to Predispose AI Agent to Learn Efficiently from Small RNA-sequencing Datasets [PDF]
arXiv, 2023 Given the increasing availability of RNA-seq data and its complex and heterogeneous nature, there has been growing interest in applying AI/machine learning methodologies to work with such data modalities. However, because omics data is characterized by high dimensionality and low sample size (HDLSS), current attempts at integrating AI in this domain ...
arxiv
Structural basis for Cas9 off-target activity [PDF]
, 2022 The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is determined by complementarity to a 20-nucleotide segment in its guide RNA.
Allain, Frédéric H-T +11 more
core +1 more source
FEBS Letters, EarlyView.Metabolic dysfunction‐associated steatotic liver disease (MASLD) affects nearly one‐third of the global population and poses a significant risk of progression to cirrhosis or liver cancer. Here, we discuss the roles of hepatic dendritic cell subtypes in MASLD, highlighting their distinct contributions to disease initiation and progression, and their ...
Camilla Klaimi +3 more
wiley +1 more source