Results 61 to 70 of about 4,450,920 (398)
Viral RNA Interactome: The Ultimate Researcher’s Guide to RNA–Protein Interactions
VirusesRNA molecules in the cell are bound by a multitude of RNA-binding proteins (RBPs) with a variety of regulatory consequences. Often, interactions with these RNA-binding proteins are facilitated by the complex secondary and tertiary structures of RNA ...
Wesley A. Hanson +2 more
doaj +1 more source
Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9. [PDF]
, 2020 CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure.
Hsu, Rohaine V +5 more
core
Molecular plant pathology, 2019 Summary Plant RNA virus‐based guide RNA (gRNA) delivery has substantial advantages compared to that of the conventional constitutive promoter‐driven expression due to the rapid and robust amplification of gRNAs during virus replication and movement.
Jiacheng Hu +9 more
semanticscholar +1 more source
Shapeshifting RNAs guide innate immunity [PDF]
Journal of Biological Chemistry, 2018 The innate immune system can distinguish between RNAs of viral and cellular origin, but the basis for this discrimination is not known. A new paper by Calderon and Conn demonstrates that conformational plasticity determines the ability of one RNA sequence to bind to and activate the pattern recognition receptor OAS1/RNase L. In identifying a novel mode
openaire +2 more sources
RNA-guided RNA silencing by an Asgard archaeal Argonaute
Nature Communications, 2023 Abstract Argonaute proteins are the central effectors of RNA-guided RNA silencing pathways in eukaryotes, playing crucial roles in gene repression and defense against viruses and transposons. Eukaryotic Argonautes are subdivided into two clades: AGOs generally facilitate miRNA- or siRNA-mediated silencing, while ...
Carolien Bastiaanssen +13 more
openaire +3 more sources
A Novel Hybrid CNN-SVR for CRISPR/Cas9 Guide RNA Activity Prediction
Frontiers in Genetics, 2020 Accurate prediction of guide RNA (gRNA) on-target efficacy is critical for effective application of CRISPR/Cas9 system. Although some machine learning-based and convolutional neural network (CNN)-based methods have been proposed, prediction accuracy ...
Guishan Zhang, Zhiming Dai, X. Dai
semanticscholar +1 more source
A Comparative Study of the Structural Dynamics of Four Terminal Uridylyl Transferases. [PDF]
, 2017 African trypanosomiasis occurs in 36 countries in sub-Saharan Africa with 10,000 reported cases annually. No definitive remedy is currently available and if left untreated, the disease becomes fatal.
Amaro, Rommie E +2 more
core +1 more source
Reciprocal control of viral infection and phosphoinositide dynamics
FEBS Letters, EarlyView.Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley +1 more source
Eukaryote-Specific Insertion Elements Control Human ARGONAUTE Slicer Activity
Cell Reports, 2013 We have solved the crystal structure of human ARGONAUTE1 (hAGO1) bound to endogenous 5′-phosphorylated guide RNAs. To identify changes that evolutionarily rendered hAGO1 inactive, we compared our structure with guide-RNA-containing and cleavage-active ...
Kotaro Nakanishi +8 more
doaj +1 more source
ACS Synthetic Biology, 2019 The CRISPR/Cas9 system is a revolutionary genome editing tool. However, in eukaryotes, search and optimization of a suitable promoter for guide RNA expression is a significant technical challenge.
Xiaomei Zheng +6 more
semanticscholar +1 more source