Determination of H+-ATPase Activity in Arabidopsis Guard Cell Protoplasts through H+-pumping Measurement and H+-ATPase Quantification [PDF]
Bio-Protocol, 2017 The opening of stomata in plants in response to blue light is driven by the plasma membrane H+-ATPase in guard cells. To evaluate the activation of the H+-ATPase in vivo, we can use H+-pumping by guard cells in response to blue light and fusicoccin.
Shota Yamauchi, Ken-ichiro Shimazaki
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SCaBP3/CBL7 negatively regulates the plasma membrane H+-ATPase and modulates hypocotyl elongation in Arabidopsis [PDF]
Plant Signaling & Behavior, 2022 The regulation of hypocotyl elongation is an important process in plant growth and development and depends on the activity of the plasma membrane (PM) H+-ATPase.
Xiao Liu +5 more
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Screening of protonstatin-1 (PS-1) analogs for improved inhibitors of plant plasma membrane H+-ATPase activity [PDF]
Frontiers in Plant Science, 2022 We previously identified protonstatin-1 (PS-1) as a selective inhibitor of plasma membrane H+-ATPase (PM H+-ATPase) activity and used it as a tool to validate the chemiosmotic model for polar auxin transport.
Yongqing Yang +8 more
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The Oligomeric State of the Plasma Membrane H+-ATPase from Kluyveromyces lactis [PDF]
Molecules, 2019 The plasma membrane H+-ATPase was purified from the yeast K. lactis. The oligomeric state of the H+-ATPase is not known. Size exclusion chromatography displayed two macromolecular assembly states (MASs) of different sizes for the solubilized enzyme. Blue
Yadira G. Ruiz-Granados +2 more
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Structure and activation mechanism of the hexameric plasma membrane H+-ATPase [PDF]
Nature Communications, 2021 The plasma membrane H+ -ATPase is responsible for maintenance of the plasma membrane potential, which provides energy for the transport of nutrients, and the plasma membrane H+ -ATPase in S.
Peng Zhao +5 more
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Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane H+-ATPase [PDF]
Nature CommunicationsPlasma membrane H+-ATPase provides the driving force for light-induced stomatal opening. However, the mechanisms underlying the regulation of its activity remain unclear.
Saashia Fuji +6 more
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Promotion and Upregulation of a Plasma Membrane Proton-ATPase Strategy: Principles and Applications
Frontiers in Plant Science, 2021 Plasma membrane proton-ATPase (PM H+-ATPase) is a primary H+ transporter that consumes ATP in vivo and is a limiting factor in the blue light-induced stomatal opening signaling pathway.
Zirong Ren +6 more
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Enhanced expression of vacuolar H+-ATPase subunit E in the roots is associated with the adaptation of Broussonetia papyrifera to salt stress. [PDF]
PLoS ONE, 2012 Vacuolar H(+)-ATPase (V-H(+)-ATPase) may play a pivotal role in maintenance of ion homeostasis inside plant cells. In the present study, the expression of V-H(+)-ATPase genes was analyzed in the roots and leaves of a woody plant, Broussonetia papyrifera,
Min Zhang +3 more
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A win-win scenario for photosynthesis and the plasma membrane H+ pump
Frontiers in Plant Science, 2022 In plants, cytosolic and extracellular pH homeostasis are crucial for various physiological processes, including the uptake of macronutrients and micronutrients, cell elongation, cell expansion, and enzyme activity. Proton (H+) gradients and the membrane
Satoru N. Kinoshita +2 more
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Wheat V-H+-ATPase subunit genes significantly affect salt tolerance in Arabidopsis thaliana. [PDF]
PLoS ONE, 2014 Genes for V-H(+)-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants.
Xiaoliang He +3 more
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