Results 1 to 10 of about 8,406 (222)
Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat. [PDF]
Mol Ther Nucleic Acids, 2016 Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Sapag A +5 more
europepmc +4 more sources
Pressure modulates the self-cleavage step of the hairpin ribozyme. [PDF]
Nat Commun, 2017 Studying the reactivity of ribozymes under extreme pressure could provide insights to optimize biocatalytic RNA design. Here, the authors show that at high pressure the transesterification step of the hairpin ribozyme self-cleavage reaction accelerates ...
Schuabb C +4 more
europepmc +4 more sources
A triplex ribozyme expression system based on a single hairpin ribozyme. [PDF]
Oligonucleotides, 2008 Triplex ribozyme (RZ) configurations allow for the individual activity of trans-acting RZs in multiple expression cassettes (multiplex), thereby increasing target cleavage relative to conventionally expressed RZs. Although hairpin RZs have been advantageously compared to hammerhead RZs, their longer size and structural features complicated triplex ...
Aquino-Jarquin G +3 more
europepmc +5 more sources
A Hairpin Ribozyme Derived Spliceozyme [PDF]
ChemBioChem, 2023 AbstractThe vast majority of RNA splicing in today‘s organisms is achieved by the highly regulated and precise removal of introns from pre‐mRNAs via the spliceosome. Here we present a model of how RNA splicing may have occurred in earlier life forms.
Jikang Zhu +2 more
openaire +3 more sources
Discovery of novel hammerhead, twister, and DVRz-associated circular RNAs in <i>Vitaceae</i>, <i>Solanaceae,</i> and <i>Rosaceae</i>. [PDF]
mSystemsViroids and viroid-like exogenous circular RNAs (ex-circRNAs) are small infectious RNA agents capable of autonomous replication within host cells, independent of any helper virus.
Raza A, Zhu Y, Deng M, Wu Q.
europepmc +2 more sources
Nucleobase catalysis in the hairpin ribozyme [PDF]
RNA, 2006 RNA catalysis is important in the processing and translation of RNA molecules, yet the mechanisms of catalysis are still unclear in most cases. We have studied the role of nucleobase catalysis in the hairpin ribozyme, where the scissile phosphate is juxtaposed between guanine and adenine bases.
Timothy J, Wilson +6 more
openaire +3 more sources
General acid-base catalysis mediated by nucleobases in the hairpin ribozyme. [PDF]
J Am Chem Soc, 2012 The catalytic mechanism by which the hairpin ribozyme accelerates cleavage or ligation of the phosphodiester backbone of RNA has been incompletely understood.
Kath-Schorr S +5 more
europepmc +4 more sources
Molecular Therapy: Nucleic Acids, 2022 The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants of hammerhead ribozymes targeting conserved structural motifs of segment 5 of influenza A virus (IAV) (+)RNA ...
Tomasz Czapik +3 more
doaj +1 more source
Life, 2022 The acquisition of functions via the elongation of nucleotides is an important factor in the development of the RNA world. In our previous study, we found that the introduction of complementary seven-membered kissing loops into inactive R3C ligase ...
Hiromi Mutsuro-Aoki, Koji Tamura
doaj +1 more source
Dual Promoters Improve the Rescue of Recombinant Measles Virus in Human Cells
Viruses, 2021 Reverse genetics is a technology that allows the production of a virus from its complementary DNA (cDNA). It is a powerful tool for analyzing viral genes, the development of novel vaccines, and gene delivery vectors.
Soroth Chey +3 more
doaj +1 more source