Results 1 to 10 of about 8,461 (225)

Hydrostatic and osmotic pressure study of the hairpin ribozyme [PDF]

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2006
The recent discovery of numerous catalytically active RNAs in various living species as well as the in vitro selection of a large series of RNA aptamers able to bind specifically various molecules such as metabolites and co-factors, emphasize the adaptability of RNAs through the plasticity of their secondary structure.
Hervé, Guy, Tobé, Sylvia, Heams, Thomas, Vergne, Jacques, Maurel, Marie-Christine   +4 more
core   +10 more sources

Mutagenesis of the hairpin ribozyme [PDF]

Nucleic Acids Research, 1994
Extensive in vitro mutagenesis studies have been performed on the hairpin ribozyme and substrate in an effort to refine the overall secondary structure of the molecule and provide further insight into what elements are essential for activity. A secondary structure consisting of four helices and five loop regions remains the basic model as originally ...
S Nesbitt, John E. Hearst, Richard H. Tritz, Pamela G. Anderson, J Monforte, Arnold Hampel   +5 more
openaire   +5 more sources

Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat [PDF]

Molecular Therapy: Nucleic Acids, 2016
Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Amalia Sapag, Thergiory Irrazábal, Lorena Lobos-González, Carlos R Muñoz-Brauning, María Elena Quintanilla, Lutske Tampier   +5 more
doaj   +2 more sources

Acquisition of Dual Ribozyme-Functions in Nonfunctional Short Hairpin RNAs through Kissing-Loop Interactions [PDF]

Life, 2022
The acquisition of functions via the elongation of nucleotides is an important factor in the development of the RNA world. In our previous study, we found that the introduction of complementary seven-membered kissing loops into inactive R3C ligase ...
Hiromi Mutsuro-Aoki, Koji Tamura
doaj   +2 more sources

Pressure modulates the self-cleavage step of the hairpin ribozyme [PDF]

Nature Communications, 2017
Studying the reactivity of ribozymes under extreme pressure could provide insights to optimize biocatalytic RNA design. Here, the authors show that at high pressure the transesterification step of the hairpin ribozyme self-cleavage reaction accelerates ...
Caroline Schuabb, Narendra Kumar, Salome Pataraia, Dominik Marx, Roland Winter   +4 more
doaj   +2 more sources

A split ribozyme system for in vivo plant RNA imaging and genetic engineering. [PDF]

Plant Biotechnol J
Summary RNA plays a central role in plants, governing various cellular and physiological processes. Monitoring its dynamic abundance provides a discerning understanding of molecular mechanisms underlying plant responses to internal (developmental) and external (environmental) stimuli, paving the way for advances in plant biotechnology to engineer crops
Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X.   +11 more
europepmc   +2 more sources

Functional Domain-Assembly in Hairpin Ribozymes [PDF]

Journal of Biochemistry, 2000
Functional structures of hairpin ribozymes have been investigated by constructing various chemically modified molecules. Domain-exchange and linker insertion experiments were performed to find active conformations of the RNA enzyme showing cleavage activity.
Ikuyo Kanzaki, Yasuo Komatsu, Eiko Ohtsuka, Shigeko Yamashita, Miho Shirai, Izumi Kumagai   +5 more
openaire   +4 more sources

Selective Nonenzymatic Formation of Biologically Common RNA Hairpins. [PDF]

Angew Chem Int Ed Engl
Template‐free RNA loop‐closing ligation selectively generates RNA tetraloops that are prevalent in biology, as demonstrated by high‐throughput sequencing. Utilizing high‐yield overhang sequences enables the efficient assembly of functional RNAs. Abstract The prebiotic formation of RNA building blocks is well‐supported experimentally, yet the emergence ...
Wu LF, Zhang J, Cornwell-Arquitt R, Hendrix DA, Radakovic A, Szostak JW.   +5 more
europepmc   +3 more sources

Site-Selective Modification and Labeling of Native RNA. [PDF]

Chemistry
The review highlights recent advancements in site‐selective modification and labeling of native RNA, an important area for the development of RNA therapeutics and fundamental RNA research. The applications, current challenges of existing methods and potential future direction of this field are also discussed.
Kha TK, Zhao Y, Zhu RY.
europepmc   +2 more sources

Intracellular Immunization against SIVmacUtilizing a Hairpin Ribozyme

Virology, 1996
A hairpin ribozyme targeting the 3' LTR region (9456) of SIVmac238 was cloned into a murine retroviral vector. This target sequence is conserved among various SIV, as well as most HIV-2, strains. The ribozyme cassette is driven from a polymerase III promoter, that of the human tRNAval gene.
Paul V. Johnson, Marina Heusch, Günter Kraus, Flossie Wong-Staal   +3 more
openaire   +4 more sources