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Characterization of Hairpin Ribozyme Reactions
2013Hairpin ribozymes are small RNA catalytic motifs naturally found in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV), and arabis mosaic virus (ArMV). The catalytic activity of the hairpin ribozyme extends to both cleavage and ligation reactions.
Preeti, Bajaj, Christian, Hammann
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The hairpin ribozyme: structure, assembly and catalysis
Current Opinion in Chemical Biology, 1998Recent studies of the hairpin ribozyme have revealed a distinct catalytic mechanism for this small RNA motif. Inner-sphere coordinated metal ions are not required, as the inert metal ion complex cobalt hexammine promotes catalysis. Detailed kinetic analyses have defined rates of individual steps in the catalytic cycle.
N G, Walter, J M, Burke
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Folding and catalysis of the hairpin ribozyme
Biochemical Society Transactions, 2005The active form of the hairpin ribozyme is brought about by the interaction of two formally unpaired loops. In a natural molecule, these are present on two adjacent arms of a four-way junction. Although activity can be obtained in molecules lacking this junction, the junction is important in the promotion of the folded state of the ribozyme under ...
T J, Wilson +3 more
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Kinetic Characterization of Hairpin Ribozyme Variants
2012Kinetic analysis of ribozyme reactions is a common method to evaluate and compare activities of catalytic RNAs. The hairpin ribozyme catalyzes the reversible cleavage of a suitable RNA substrate at a specific site. Hairpin ribozyme variants as an allosteric ribozyme responsive to flavine mononucleotide and a hairpin-derived twin ribozyme that catalyzes
Bettina, Appel +3 more
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Self-association of adenine-dependent hairpin ribozymes
European Biophysics Journal, 2007Hairpin ribozymes are flexible molecules that catalyse reversible self-cleavage after the docking of two independently folded internal loops, A and B. The activities, self-association and structures in solution of two 85 base adenine-dependent hairpin ribozymes (ADHR1 and ADHR2) were studied by native gel electrophoresis, analytical centrifugation, and
Li, Yan-Li +5 more
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1994
RNA enzymes, or ribozymes, are fascinating molecules with novel biochemical properties. Since the discovery of ribozymes in 1981, biological catalysis by RNA has been found to be at the heart of critical steps in gene expression, including RNA splicing, endonucleolytic RNA processing, and possibly ribosomal peptide bond formation (Sheldon et al.
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RNA enzymes, or ribozymes, are fascinating molecules with novel biochemical properties. Since the discovery of ribozymes in 1981, biological catalysis by RNA has been found to be at the heart of critical steps in gene expression, including RNA splicing, endonucleolytic RNA processing, and possibly ribosomal peptide bond formation (Sheldon et al.
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Structure and function of the hairpin ribozyme
Journal of Molecular Biology, 2000The hairpin ribozyme belongs to the family of small catalytic RNAs that cleave RNA substrates in a reversible reaction that generates 2',3'-cyclic phosphate and 5'-hydroxyl termini. The hairpin catalytic motif was discovered in the negative strand of the tobacco ringspot virus satellite RNA, where hairpin ribozyme-mediated self-cleavage and ligation ...
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Design and Function of Triplex Hairpin Ribozymes
2010Triplex ribozymes allow for the individual activity of multiple trans-acting ribozymes producing higher target cleavage relative to tandem-expressed RZs. A triplex expression system based on a single hairpin ribozyme for the multiple expression (multiplex) vectors can be engineered to target RNAs with single or multiple antisense-accessible sites ...
Guillermo, Aquino-Jarquin +2 more
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Impedimetric Detection of Hairpin Ribozyme Activity
Electroanalysis, 2010AbstractElectrochemical impedance spectroscopy is successfully utilized for label‐free monitoring of the cleavage reaction of a RNA substrate by a complementary hairpin ribozyme. The formation of the RNA substrate/ribozyme complex is increasing the negative charge at the interface and is modulating the kinetic of the redox conversion of a negatively ...
Katarzyna Piekielska +4 more
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The FEBS Journal, 2005
In recent years major progress has been made in elucidating the mechanism and structure of catalytic RNA molecules, and we are now beginning to understand ribozymes well enough to turn them into useful tools. Work in our laboratory has focused on the development of twin ribozymes for site‐specific RNA sequence alteration.
Sergei A, Ivanov +2 more
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In recent years major progress has been made in elucidating the mechanism and structure of catalytic RNA molecules, and we are now beginning to understand ribozymes well enough to turn them into useful tools. Work in our laboratory has focused on the development of twin ribozymes for site‐specific RNA sequence alteration.
Sergei A, Ivanov +2 more
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